CAJ | 학술논문

背景:新型生物全降解聚左旋乳酸/无定形磷酸钙支架展现了良好的应用前景,但支架材料植入后是否引起周围组织钙化尚不明确.目的:观察聚左旋乳酸/无定形磷酸钙支架植入SD大鼠肌内组织后对周围组织钙化的影响.方法:将48只SD大鼠随机均分为实验组与对照组,在实验组大鼠背部肌肉组织中植入聚左旋乳酸/无定形磷酸钙支架,在对照组大鼠背部肌肉组织中植入聚左旋乳酸支架.植入后1,2,4,12周,分别检测肝肾功能及血钙、磷、碱性磷酸酶水平;取支架及周围肌肉组织进行苏木精-伊红染色、钙化Von kossa染色、碱性磷酸酶染色及免疫组织化学核因子κB染色,Western blot检测周围肌肉组织白细胞介素6、骨形成蛋白2水平,并检测组织匀浆钙、碱性磷酸酶含量.结果与结论:两组支架植入未造成大鼠肝肾功能的改变,并且随着植入时间延长亦无明显变化.实验组植入后2,4,12周的白细胞介素6表达少于对照组(P < 0.05),植入后4,12周的核因子κB阳性表达指数、骨形成蛋白2、炎症细胞计数少于对照组(P < 0.05),植入后12周的支架周围组织钙含量低于对照组(P < 0.05);两组周围组织碱性磷酸酶含量表达、VonKossa 钙化相关染色及血中 Ca、P、碱性磷酸酶含量对比均无明显差别(P > 0.05).表明聚左旋乳酸/无定形磷酸钙支架具有良好的安全性及生物相容性,未引起周围组织钙化.
배경:신형생물전강해취좌선유산/무정형린산개지가전현료량호적응용전경,단지가재료식입후시부인기주위조직개화상불명학.목적:관찰취좌선유산/무정형린산개지가식입SD대서기내조직후대주위조직개화적영향.방법:장48지SD대서수궤균분위실험조여대조조,재실험조대서배부기육조직중식입취좌선유산/무정형린산개지가,재대조조대서배부기육조직중식입취좌선유산지가.식입후1,2,4,12주,분별검측간신공능급혈개、린、감성린산매수평;취지가급주위기육조직진행소목정-이홍염색、개화Von kossa염색、감성린산매염색급면역조직화학핵인자κB염색,Western blot검측주위기육조직백세포개소6、골형성단백2수평,병검측조직균장개、감성린산매함량.결과여결론:량조지가식입미조성대서간신공능적개변,병차수착식입시간연장역무명현변화.실험조식입후2,4,12주적백세포개소6표체소우대조조(P < 0.05),식입후4,12주적핵인자κB양성표체지수、골형성단백2、염증세포계수소우대조조(P < 0.05),식입후12주적지가주위조직개함량저우대조조(P < 0.05);량조주위조직감성린산매함량표체、VonKossa 개화상관염색급혈중 Ca、P、감성린산매함량대비균무명현차별(P > 0.05).표명취좌선유산/무정형린산개지가구유량호적안전성급생물상용성,미인기주위조직개화.
BACKGROUND:Novel fuly biodegradable poly-L-lactic acid/amorphous calcium phosphate (PLLA/ACP) scaffold shows a good prospect of application, but whether the scaffold material has impact on the surrounding tissue calcification is unknown. OBJECTIVE: To observe the influence of PLLA/ACP scaffold material on the calcification of surrounding tissue after implantation of PLLA/ACP scaffold into rats. METHODS:A total of 48 SD rats were divided into experimental group and control group randomly. The experimental group was implanted with PLLA/ACP scaffold material, while the control group was implanted with PLLA scaffold material. At 1, 2, 4, 12 weeks after implantation, the liver function, kidney function and concentrations of calcium, phosphorus, alkaline phosphatase in serum were detected; the muscle tissue around the scaffold was colected for hematoxylin-eosin staining, Von Kossa staining, alkaline phosphatase staining and immunohistochemical staining of nuclear factor-kappa B. Then, western blot assay was used to detect the contents of interleukin-6, bone morphogenetic protein-2, and meanwhile, the contents of calcium and alkaline phosphatase in tissue homogenate were measured. RESULTS AND CONCLUSION:There was no significant difference in either group about the liver and kidney functions at each time. The content of interleukin-6 in the experimental group was less than that in the controlgroup at 2, 4 and 12 weeks after implantation (P < 0.05). The positive expression of nuclear factor-kappa B, bone morphogenetic protein-2 and inflammatory cel count in the experimental group were less than those in the control group at 4 and 12 weeks after implantation (P < 0.05). The content of calcium in the experimental group was less than that in the control group at 12 weeks after implantation (P < 0.05). No difference was found in the expression of alkaline phosphate, the Von Kossa staining and the content of calcium, phosphorus, alkaline phosphatase in the muscle tissue around the scaffold between the two groups (P > 0.05). These findings indicate that the PLLA/ACP scaffold has a good biocompatibility and biological security, which cannot induce peripheral tissue calcification.