CAJ | 학술논문

背景:高分子材料聚四氟乙烯膨体作为隆鼻填充材料具有耐腐蚀、化学性质稳定等优点,但其线膨胀系数较大,易引发感染及排异反应,故应用有一定局限性.目的:对比聚四氟乙烯和聚四氟乙烯联合Ⅰ型胶原作为隆鼻填充材料的细胞毒性、埋植后的炎性浸润及体内生物相容性.方法:采用MTT法检测聚四氟乙烯浸提液和聚四氟乙烯联合Ⅰ型胶原浸提液培养L929细胞的细胞增殖.采用电子显微镜观察聚四氟乙烯浸提液和聚四氟乙烯联合Ⅰ型胶原浸提液培养L929细胞后的细胞生长情况.将聚四氟乙烯和聚四氟乙烯联合Ⅰ型胶原材料分别埋置于新西兰白兔鼻背筋膜下7 d,苏木精-伊红染色观察鼻黏膜上皮组织炎性浸润情况.兔耳缘静脉分别注射聚四氟乙烯浸提液和聚四氟乙烯联合Ⅰ型胶原浸提液后,观察兔的全身毒性、过敏、热源反应及死亡情况.结果与结论:作为隆鼻填充材料,聚四氟乙烯联合Ⅰ型胶原材料在细胞毒性、埋植后的炎性浸润方面均优于单纯聚四氟乙烯材料(P < 0.05);兔耳缘静脉注射聚四氟乙烯联合Ⅰ型胶原材料后发生的过敏反应、热源反应少于注射单纯聚四氟乙烯材料(P < 0.05).表明聚四氟乙烯联合Ⅰ型胶原作为隆鼻填充材料具有良好的生物相容性.
배경:고분자재료취사불을희팽체작위륭비전충재료구유내부식、화학성질은정등우점,단기선팽창계수교대,역인발감염급배이반응,고응용유일정국한성.목적:대비취사불을희화취사불을희연합Ⅰ형효원작위륭비전충재료적세포독성、매식후적염성침윤급체내생물상용성.방법:채용MTT법검측취사불을희침제액화취사불을희연합Ⅰ형효원침제액배양L929세포적세포증식.채용전자현미경관찰취사불을희침제액화취사불을희연합Ⅰ형효원침제액배양L929세포후적세포생장정황.장취사불을희화취사불을희연합Ⅰ형효원재료분별매치우신서란백토비배근막하7 d,소목정-이홍염색관찰비점막상피조직염성침윤정황.토이연정맥분별주사취사불을희침제액화취사불을희연합Ⅰ형효원침제액후,관찰토적전신독성、과민、열원반응급사망정황.결과여결론:작위륭비전충재료,취사불을희연합Ⅰ형효원재료재세포독성、매식후적염성침윤방면균우우단순취사불을희재료(P < 0.05);토이연정맥주사취사불을희연합Ⅰ형효원재료후발생적과민반응、열원반응소우주사단순취사불을희재료(P < 0.05).표명취사불을희연합Ⅰ형효원작위륭비전충재료구유량호적생물상용성.
BACKGROUND:Polytetrafluoroethylene (PTEE) as a nose filer material has advantages on the resistant to corrosion, chemical stability and so on. However, its linear expansion coefficient is large easily leading to infection and rejection, and its application has some limitations. OBJECTIVE:To compare the cel toxicity, inflammatory infiltrates and biocompatibility indexesin vivo between PTEE and PTEE combined with type Ⅰ colagen. METHODS: MTT method was used to detect the relative proliferation rate of L929 cels cultured with PTEE extract or PTEE combined with type Ⅰ colagen extract; an electron microscope was used to observe the cel growth. PTEE or PTEE combined with type Ⅰ colagen was embedded under the nasal dorsal fascia of New Zealand white rabbits for 7 days, and hematoxylin-eosin staining was used to detect whether nasal mucosa epithelial tissue had inflammatory infiltration. Systemic toxicity, alergies, pyrogen reaction and deaths of rabbits were observed after injection of two kinds of material extracts by ear vein. RESULTS AND CONCLUSION: The cytotoxicity and inflammatory infiltration were milder in PTEE combined with type Ⅰ colagen group than PTEE group (P < 0.05). The alergic reactions and pyrogen reactions were fewer in PTEE combined with type Ⅰ colagen group than PTEE group (P < 0.05). These findings indicate that PTEE combined with type Ⅰ colagen as the nose filer material has better biocompatibility.