中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
30期
4769-4773
,共5页
生物材料%骨生物材料%纳米羟基磷灰石%聚酰胺%成骨能力%广东省自然科学基金
生物材料%骨生物材料%納米羥基燐灰石%聚酰胺%成骨能力%廣東省自然科學基金
생물재료%골생물재료%납미간기린회석%취선알%성골능력%광동성자연과학기금
背景:作为骨修复重建材料,纳米羟基磷灰石具有良好生物相容性及骨传导性,但临床上中单一使用纳米羟基磷灰石尚存在许多不足.目的:观察纳米羟基磷灰石/聚酰胺材料的体内成骨能力.方法:取 24 只新西兰大白兔,进行纳米羟基磷灰石/聚酰胺人工肱骨头置换,分别于置换后 3,6,12,24周,进行X射线观察、组织学观察.结果与结论:①X 射线观察:不同时间点材料上端皮质均未出现骨皮质变薄、异位骨化等发生,纳米羟基磷灰石/聚酰胺材料无碎裂迹象,材料四周皮质均可见模糊界面,密度随着时间的增加而增加.②组织学观察:置换后3周,可见大量细胞,包括间充质细胞、单核巨噬细胞等;置换后6周,仍可见界膜内的大量纤维组织、成纤维细胞、单核巨噬细胞,而软骨细胞和成骨细胞分布较少;置换后12周,大范围原始骨小梁开始形成且多呈扁平状,排列整齐有序;置换后24周,组织界膜被骨细胞充斥,骨小梁表面的细胞较规则,骨组织原始细胞开始转变为板层状骨.说明纳米羟基磷灰石/聚酰胺材料具有良好的成骨能力.
揹景:作為骨脩複重建材料,納米羥基燐灰石具有良好生物相容性及骨傳導性,但臨床上中單一使用納米羥基燐灰石尚存在許多不足.目的:觀察納米羥基燐灰石/聚酰胺材料的體內成骨能力.方法:取 24 隻新西蘭大白兔,進行納米羥基燐灰石/聚酰胺人工肱骨頭置換,分彆于置換後 3,6,12,24週,進行X射線觀察、組織學觀察.結果與結論:①X 射線觀察:不同時間點材料上耑皮質均未齣現骨皮質變薄、異位骨化等髮生,納米羥基燐灰石/聚酰胺材料無碎裂跡象,材料四週皮質均可見模糊界麵,密度隨著時間的增加而增加.②組織學觀察:置換後3週,可見大量細胞,包括間充質細胞、單覈巨噬細胞等;置換後6週,仍可見界膜內的大量纖維組織、成纖維細胞、單覈巨噬細胞,而軟骨細胞和成骨細胞分佈較少;置換後12週,大範圍原始骨小樑開始形成且多呈扁平狀,排列整齊有序;置換後24週,組織界膜被骨細胞充斥,骨小樑錶麵的細胞較規則,骨組織原始細胞開始轉變為闆層狀骨.說明納米羥基燐灰石/聚酰胺材料具有良好的成骨能力.
배경:작위골수복중건재료,납미간기린회석구유량호생물상용성급골전도성,단림상상중단일사용납미간기린회석상존재허다불족.목적:관찰납미간기린회석/취선알재료적체내성골능력.방법:취 24 지신서란대백토,진행납미간기린회석/취선알인공굉골두치환,분별우치환후 3,6,12,24주,진행X사선관찰、조직학관찰.결과여결론:①X 사선관찰:불동시간점재료상단피질균미출현골피질변박、이위골화등발생,납미간기린회석/취선알재료무쇄렬적상,재료사주피질균가견모호계면,밀도수착시간적증가이증가.②조직학관찰:치환후3주,가견대량세포,포괄간충질세포、단핵거서세포등;치환후6주,잉가견계막내적대량섬유조직、성섬유세포、단핵거서세포,이연골세포화성골세포분포교소;치환후12주,대범위원시골소량개시형성차다정편평상,배렬정제유서;치환후24주,조직계막피골세포충척,골소량표면적세포교규칙,골조직원시세포개시전변위판층상골.설명납미간기린회석/취선알재료구유량호적성골능력.
BACKGROUND:As a bone reconstruction material, nano-hydroxyapatite has good biocompatibility and osteoconduction, but the clinical use of nano-hydroxyapatite alone stil has many deficiencies. OBJECTIVE:To explore the in vivo osteogenic capability of nano-hydroxyapatite/ polyamide composites. METHODS: Twenty-four New Zealand white rabbits were subjected to humeral head replacement using nano-hydroxyapatite/polyamide composite material. X-ray observation and histological observation were done at 3, 6, 12, 24 weeks after replacement. RESULTS AND CONCLUSION: (1) X-ray observation: No thinned cortical bone and ectopic ossification occurred on the upper end of the composite material at different time, and the nano-hydroxyapatite/polyamide material had no signs of fragmentation. The cortical bone around the composite material was fuzzy, and the bone mineral density was increased with time. (2) Histological observation: At 3 weeks after replacement, a large number of cels could be visible, including mesenchymal stem cels and mononuclear macrophages. At 6 weeks after replacement, a large amount of fibrous tissues, fibroblasts and mononuclear macrophages stil existed in the boundary membrane, but chondrocytes and osteoblasts distributed less. At 12 weeks after replacement, a wide range of original trabecular bone began to form and were mostly flat that arranged regularly. At 24 weeks after replacement, the boundary membrane was ful of bone cels, but the cels on the surface of trabecular bone were relatively regular and primitive cels in the bone tissue began to transform into the lamelar bone. These findings indicate that the nano-hydroxyapatite/polyamide material has good osteogenic capability.