CAJ | 학술논문

目的探讨微小RNA(miRNA,miR)-192在Ⅱ、Ⅲ期结直肠癌组织中表达及其与患者术后复发转移的关系.方法收集行结直肠癌根除术的Ⅱ、Ⅲ期患者新鲜标本119例,其中,3年内发生复发转移45例(转移复发组),未出现复发转移74例(未转移复发组).同时,取40例确证无癌细胞的癌旁组织作为对照组,利用反转录实时定量聚合酶链反应(PCR)检测肿瘤及癌旁组织中miR-192表达,分析其对结直肠癌患者术后复发转移的影响.结果 miR-192在结直肠癌患者肿瘤组织中相对表达量(0.27 ±0.06)显著低于癌旁组织中的(0.56±0.12),差异有统计学意义(P＜0.05),miR-192在转移复发组患者肿瘤组织中相对表达量(0.13±0.04)显著低于未转移复发组(0.32±0.09),差异有统计学意义(P＜0.05);miR-192高表达组患者术后复发率为29.5％,低于低表达组的61.3％,差异有统计学意义(P＜0.05);转移复发组患者中miR-192高表达者术后中位无复发生存时间为18.3个月,长于低表达者的14.7个月,差异有统计学意义(P＜0.05);单因素生存分析显示,miR-192表达降低与患者术后无复发生存时间缩短有关,且在Ⅲ期患者中更为明显(P＜0.05),Cox比例风险模型分析显示,miR-192低表达[相对危险度(RR) =0.402,95％可信区间(CI):0.312 ～0.803,P＜0.05]是影响结直肠癌患者术后无复发生存期的独立风险因素.结论 miR-192在结直肠癌中呈低表达,且与患者术后肿瘤复发转移密切相关,是影响结直肠癌术后无复发生存期的独立危险因素.
목적 탐토미소RNA(miRNA,miR)-192재Ⅱ、Ⅲ기결직장암조직중표체급기여환자술후복발전이적관계.방법 수집행결직장암근제술적Ⅱ、Ⅲ기환자신선표본119례,기중,3년내발생복발전이45례(전이복발조),미출현복발전이74례(미전이복발조).동시,취40례학증무암세포적암방조직작위대조조,이용반전록실시정량취합매련반응(PCR)검측종류급암방조직중miR-192표체,분석기대결직장암환자술후복발전이적영향.결과 miR-192재결직장암환자종류조직중상대표체량(0.27 ±0.06)현저저우암방조직중적(0.56±0.12),차이유통계학의의(P＜0.05),miR-192재전이복발조환자종류조직중상대표체량(0.13±0.04)현저저우미전이복발조(0.32±0.09),차이유통계학의의(P＜0.05);miR-192고표체조환자술후복발솔위29.5％,저우저표체조적61.3％,차이유통계학의의(P＜0.05);전이복발조환자중miR-192고표체자술후중위무복발생존시간위18.3개월,장우저표체자적14.7개월,차이유통계학의의(P＜0.05);단인소생존분석현시,miR-192표체강저여환자술후무복발생존시간축단유관,차재Ⅲ기환자중경위명현(P＜0.05),Cox비례풍험모형분석현시,miR-192저표체[상대위험도(RR) =0.402,95％가신구간(CI):0.312 ～0.803,P＜0.05]시영향결직장암환자술후무복발생존기적독립풍험인소.결론 miR-192재결직장암중정저표체,차여환자술후종류복발전이밀절상관,시영향결직장암술후무복발생존기적독립위험인소.
Objective To investigate the expression of microRNA (miRNA,miR)-192 in Ⅱ-and Ⅲ-stage colorectal cancer tissues and its relationship with postoperative recurrence and metastasis.Methods 119 cases of fresh specimens from patients with colorectal cancer of stages Ⅱ and Ⅲ undergoing colorectal cancer eradication in Henan Tumor Hospital were selected from January 2010 to December 2010.Forty-five cases had recurrence within three years (metastasis and recurrence group),and 74 cases had no recurrence (non-metastasis and recurrence group).At the same time,40 cases of adjacent non-cancerous tissues were selected as the control group.The expression levels of miR-192 in the tumor and adjacent non-cancerous tissues were detected by reverse transcription quantitative real-time polymerase chain reaction.Its impacts on postoperative recurrence of colorectal cancer patients after surgery were analyzed.Results The relative expression level of miR-192 in the tumor tissues of patients with colorectal cancer was (0.27 ±0.06),which was significantly lower than (0.56 ±0.12) in the adjacent non-cancerous tissues (P ＜ 0.05).The relative expression level of miR-192 in the tumor tissues of the metastasis and recurrence group was (0.13 ±0.04),which was significantly lower than (0.32 ±0.09) in the non-metastasis and-recurrence group (P ＜ 0.05).The recurrence rate in the high miR-192 expression group was 29.5％,which was significantly lower than the low miR-192 expression group (P ＜ 0.05).The median relapse-free survival of the patients with high miR-192 expression in metastasis and recurrence group was 18.3 months,which was significantly longer than 14.7 months in the patients with low miR-192 expression (P ＜ 0.05).Univariate analysis showed that miR-192 low expression was related with the reduction of postoperative recurrence-free survival time,more pronounced in patients with stage Ⅲ (P ＜ 0.05).Cox proportional hazards model analysis showed that the low expression of miR-192 [relative risk (RR) =0.402,95 ％ confidence interval (CI):0.312-0.803,P ＜ 0.05] was an independent risk factor influencing postoperative relapse-free survival of colorectal cancer patients.Conclusion miR-192 was lowly expressed in colorectal cancer,and was closely associated with postoperative recurrence of patients.It was an independent risk factor influencing postoperative recurrence-free survival of patients with colorectal cancer.