CAJ | 학술논문

研究不同生长阶段肉羊骨骼肌肌球蛋白重链（MyHC）基因相对表达量的变化规律，实验选择自然放牧条件下的1、3、6、9、12、18月龄的乌珠穆沁羊，屠宰后采取股二头肌、背最长肌，采用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳（sodium dodecyl sulfate polyacrylamide gel electropheresis，SDS-PAGE）法分离骨骼肌中肌球蛋白重链异构体，利用实时荧光定量聚合酶链式反应（polymerase chain reaction，PCR）技术测定MyHC基因的相对表达量并探讨其变化规律。结果表明：从SDS-PAGE图中可以清晰地观察到4条条带分别为Ⅱa、Ⅱx、Ⅱb型与Ⅰ型。其中乌珠穆沁羊股二头肌和背最长肌中MyHC的Ⅰ型和Ⅱa型基因的相对表达量均高于Ⅱb型基因的相对表达量。
연구불동생장계단육양골격기기구단백중련（MyHC）기인상대표체량적변화규률，실험선택자연방목조건하적1、3、6、9、12、18월령적오주목심양，도재후채취고이두기、배최장기，채용십이완기광산납-취병희선알응효전영（sodium dodecyl sulfate polyacrylamide gel electropheresis，SDS-PAGE）법분리골격기중기구단백중련이구체，이용실시형광정량취합매련식반응（polymerase chain reaction，PCR）기술측정MyHC기인적상대표체량병탐토기변화규률。결과표명：종SDS-PAGE도중가이청석지관찰도4조조대분별위Ⅱa、Ⅱx、Ⅱb형여Ⅰ형。기중오주목심양고이두기화배최장기중MyHC적Ⅰ형화Ⅱa형기인적상대표체량균고우Ⅱb형기인적상대표체량。
This study was aimed to investigate the changes in the relative expression level of myosin heavy chain (MyHC) gene from skeletal muscle during the growth of Wuzhumuqin sheep. Free-grazing Wuzhumuqin sheep were slaughtered at 1, 3, 6, 9, 12 and 18 months of age and thebiceps femoris andlongissimus dorsi muscles were excised. MyHC isoforms were separated from the muscles by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and the relative expression level ofMyHC gene was measured by real-time polymerase chain reaction (PCR). The results showed that four clear bands representing typeⅡa, typeⅡx, typeⅡb and typeⅠ, respectively, were observed in the electrophoretic pattern. The expression ofMyHC typeⅠ gene and typeⅡa inbiceps femoris andlongissimus dorsiwas higher than that ofMyHC typeⅡb gene. Understanding theMyHC gene expression in skeletal muscle during the growth of sheep is of great practical importance for improving meat quality and post-mortem physiological and biochemical characteristics.