中华神经创伤外科电子杂志
中華神經創傷外科電子雜誌
중화신경창상외과전자잡지
Chinese Journal of Neurotraumatic Surgery (Electronic Edition)
2015年
3期
36-40
,共5页
刘光杰%刘家传%王金标%杨艳艳%张星%王春琳%汤宏%徐燊
劉光傑%劉傢傳%王金標%楊豔豔%張星%王春琳%湯宏%徐燊
류광걸%류가전%왕금표%양염염%장성%왕춘림%탕굉%서신
颅脑外伤%缺氧预处理%TrxR2%CHOP%超微结构
顱腦外傷%缺氧預處理%TrxR2%CHOP%超微結構
로뇌외상%결양예처리%TrxR2%CHOP%초미결구
Traumatic brain injury%Hypoxic preconditioning%TrxR2%CHOP%Ultra-structure
目的:探讨缺氧预处理对颅脑外伤大鼠皮层TrxR2、CHOP表达及神经元细胞超微结构改变的影响。方法48只SD大鼠随机分成对照(Con)组、缺氧预处理(HPC)组、单纯外伤(TBI)组和缺氧预处理颅脑外伤(HPCT)组。采用改良的Feeney’ s自由落体装置制作颅脑损伤大鼠模型,预先在低压氧舱内连续处理3 d(-50 kPa、3 h/d)制作缺氧预处理模型。采用HE染色法和新鲜标本制作超薄切片,分别在光镜和电镜下观察各组大鼠皮层脑组织的大体结构和神经元细胞超微结构改变。qRT-PCR和Western blotting法检测挫伤区周围皮层TrxR2、CHOP mRNA和蛋白的表达变化。结果 Con组和HPC组病理改变无明显差异,HPCT组较TBI组大鼠病理改变轻,神经元细胞超微结构受损明显减轻。HPCT与TBI组比较,TrxR2 mRNA和蛋白表达显著上调, CHOP mRNA和蛋白表达显著下调,差异均具有统计学意义(均P<0.05);Con与HPC组比较,差异无统计学意义(P>0.05)。结论缺氧预处理可明显减轻颅脑外伤大鼠皮层神经元细胞形态学改变,这可能与上调TrxR2、下调CHOP的表达有密切关系,从而对神经元细胞起保护作用。
目的:探討缺氧預處理對顱腦外傷大鼠皮層TrxR2、CHOP錶達及神經元細胞超微結構改變的影響。方法48隻SD大鼠隨機分成對照(Con)組、缺氧預處理(HPC)組、單純外傷(TBI)組和缺氧預處理顱腦外傷(HPCT)組。採用改良的Feeney’ s自由落體裝置製作顱腦損傷大鼠模型,預先在低壓氧艙內連續處理3 d(-50 kPa、3 h/d)製作缺氧預處理模型。採用HE染色法和新鮮標本製作超薄切片,分彆在光鏡和電鏡下觀察各組大鼠皮層腦組織的大體結構和神經元細胞超微結構改變。qRT-PCR和Western blotting法檢測挫傷區週圍皮層TrxR2、CHOP mRNA和蛋白的錶達變化。結果 Con組和HPC組病理改變無明顯差異,HPCT組較TBI組大鼠病理改變輕,神經元細胞超微結構受損明顯減輕。HPCT與TBI組比較,TrxR2 mRNA和蛋白錶達顯著上調, CHOP mRNA和蛋白錶達顯著下調,差異均具有統計學意義(均P<0.05);Con與HPC組比較,差異無統計學意義(P>0.05)。結論缺氧預處理可明顯減輕顱腦外傷大鼠皮層神經元細胞形態學改變,這可能與上調TrxR2、下調CHOP的錶達有密切關繫,從而對神經元細胞起保護作用。
목적:탐토결양예처리대로뇌외상대서피층TrxR2、CHOP표체급신경원세포초미결구개변적영향。방법48지SD대서수궤분성대조(Con)조、결양예처리(HPC)조、단순외상(TBI)조화결양예처리로뇌외상(HPCT)조。채용개량적Feeney’ s자유락체장치제작로뇌손상대서모형,예선재저압양창내련속처리3 d(-50 kPa、3 h/d)제작결양예처리모형。채용HE염색법화신선표본제작초박절편,분별재광경화전경하관찰각조대서피층뇌조직적대체결구화신경원세포초미결구개변。qRT-PCR화Western blotting법검측좌상구주위피층TrxR2、CHOP mRNA화단백적표체변화。결과 Con조화HPC조병리개변무명현차이,HPCT조교TBI조대서병리개변경,신경원세포초미결구수손명현감경。HPCT여TBI조비교,TrxR2 mRNA화단백표체현저상조, CHOP mRNA화단백표체현저하조,차이균구유통계학의의(균P<0.05);Con여HPC조비교,차이무통계학의의(P>0.05)。결론결양예처리가명현감경로뇌외상대서피층신경원세포형태학개변,저가능여상조TrxR2、하조CHOP적표체유밀절관계,종이대신경원세포기보호작용。
Objective To investigate the effects of hypoxic preconditioning on the expression of TrxR2、CHOP and the ultra-structure of the neuronal cells of rats suffered traumatic brain injury. Methods Forty eight Sprague Dawley rats were randomly divided into control group (Con), hypoxic preconditioning group (HPC), traumatic brain injury group (TBI) and hypoxic preconditioning traumatic brain injury group (HPCT). The traumatic brain injury model of rats were made by the Feeney’ s improved equipment and hypoxic preconditioning model were made by Hypobaric chamber for successive 3 days (-50 kPa、3 h/d). Staining HE and making ultra-thin slicing by fresh specimen to observe the potential change of general structure and ultra-structure of rats’ brain tissue of each group respectively under light microscope and electron microscope. Expressions of TrxR2、CHOP mRNA and proteins were detected in the brain cortex around the contusion area by qRT-PCR and Western blotting. Results The pathologic change has no difference between Con gourp and HPC group. HPCT group has a lighter pathologic change than the TBI group.The expressions of TrxR2 mRNA and protein was significantly higher in the brain cortex in the HPCT group than the TBI group, the difference between the two group was significant (P<0.05), the expressions of CHOP mRNA and protein was significantly lower in the brain cortex in the HPCT group than the TBI group, the difference between the two group was significant (P<0.05), while the difference between the Con group and the HPC group was not significant (P>0.05). Conclusion Hypoxia preconditioning can obviously reduce the craniocerebral trauma rats cortex neuron cell morphology change,which has a close relationship with the upregulated expression of TrxR2 and the downregulated expression of CHOP, in order to protection of neurons.