CAJ | 학술논문

目的：检测血液制品及原料血浆中人细小病毒B19，分析污染状况，以评估人细小病毒B19危险性。方法对单人原料血浆、混合原料血浆、4种类型的血液制品提取核酸后分别进行B19病毒核酸实时定量PCR检测筛查，对筛查结果进行数据统计分析。结果实验检测到个人血浆阳性率为0.267％、混合血浆感染5.45％、静注人免疫球蛋白感染0、人纤维蛋白原感染5.67％、人凝血因子Ⅷ和人凝血酶原复合物阳性率分别为7.41％和10.3％。结论尽管国内在血液制品生产工艺中使用了灭活/去除B19病毒的方法，但在本研究中筛查的原料血浆及血液制品中仍检测出了B19 DNA。
목적：검측혈액제품급원료혈장중인세소병독B19，분석오염상황，이평고인세소병독B19위험성。방법대단인원료혈장、혼합원료혈장、4충류형적혈액제품제취핵산후분별진행B19병독핵산실시정량PCR검측사사，대사사결과진행수거통계분석。결과실험검측도개인혈장양성솔위0.267％、혼합혈장감염5.45％、정주인면역구단백감염0、인섬유단백원감염5.67％、인응혈인자Ⅷ화인응혈매원복합물양성솔분별위7.41％화10.3％。결론진관국내재혈액제품생산공예중사용료멸활/거제B19병독적방법，단재본연구중사사적원료혈장급혈액제품중잉검측출료B19 DNA。
Objective To test human bocavirus B19 in blood products and source plasma, and evaluate the danger of human bocavirus B19 by analyzing its contamination status.Methods Real-time quantitative PCR testing and screening of B19 nucleic acid was made after extracting nucleic acid from single source plasma, mixed source plasma and four types' blood products, and the screening results were statistically analyzed. Results The results were tested in laboratory including 0.267% positive rate of single plasma, 5.15% of contamination in mixed plasma, 0 of contamination in intravenous injection of human immunoglobulin, 5.67% of contamination in human fibrinogen, 7.41% positive rate of human coagulation factor Ⅷ and 10.3% positive rate of human prothrombin complex. Conclusion Although the methods to inactivate or eliminate B19 virus in production process of blood products, B19 DNA is still tested in the screening source plasma and blood products in this research.