CAJ | 학술논문

目的：对骨肉瘤患者行磁共振动态增扫描，探讨MR动态增强诸参数与穿刺骨组织内免疫组化因子表达的相关性。材料与方法对30例骨肉瘤初诊患者，经肘静脉注射钆双胺后行MR动态增强扫描，获取骨肉瘤动态增强参数，然后对相关区域进行穿刺活检，测定活检组织的HIF-1α、MVD和VEGF表达水平。对增强参数和免疫组化因子进行对比和相关研究。结果活检部位Slope、Smax、SImax与HIF-1α表达呈正相关关系(γ分别为0.894、0.832、0.681， P＜0.05)，与MVD也呈正相关关系(γ分别为0.629、0.739、0.663，P＜0.05)，与VEGF表达呈正相关关系(γ分别为0.876、0.829、0.627，P＜0.05)；T1 onset、TP与HIF-1α表达呈负相关关系(γ分别为－0.692、－0.667，P＜0.05)，与MVD也呈负相关关系(γ分别为－0.574、－0.835，P＜0.05)，与VEGF表达呈负相关关系(γ分别为－0.907、－0.830，P＜0.05)。结论穿刺活检部位骨肉瘤MRI动态增强参数与免疫组化三因子表达有统计学相关性，与组织乏氧因子明显相关，动态增强扫描结果能为检测骨肉瘤组织内乏氧与血管生成提供依据。
목적：대골육류환자행자공진동태증소묘，탐토MR동태증강제삼수여천자골조직내면역조화인자표체적상관성。재료여방법대30례골육류초진환자，경주정맥주사구쌍알후행MR동태증강소묘，획취골육류동태증강삼수，연후대상관구역진행천자활검，측정활검조직적HIF-1α、MVD화VEGF표체수평。대증강삼수화면역조화인자진행대비화상관연구。결과활검부위Slope、Smax、SImax여HIF-1α표체정정상관관계(γ분별위0.894、0.832、0.681， P＜0.05)，여MVD야정정상관관계(γ분별위0.629、0.739、0.663，P＜0.05)，여VEGF표체정정상관관계(γ분별위0.876、0.829、0.627，P＜0.05)；T1 onset、TP여HIF-1α표체정부상관관계(γ분별위－0.692、－0.667，P＜0.05)，여MVD야정부상관관계(γ분별위－0.574、－0.835，P＜0.05)，여VEGF표체정부상관관계(γ분별위－0.907、－0.830，P＜0.05)。결론천자활검부위골육류MRI동태증강삼수여면역조화삼인자표체유통계학상관성，여조직핍양인자명현상관，동태증강소묘결과능위검측골육류조직내핍양여혈관생성제공의거。
AbstractObjective: To investigate the correlation between various internal parameters of dynamic contrast-enhanced MR and the immunohistochemistry factor expression of revelevant site by biopsy.Materials and Methods:Thirty cases of newly diagnosed patients with osteosarcoma were choosen. All cases underwent dynamic contrast-enhanced MR scanning after injecting gadodiamide through the cubital vein. The regional enhancement curve and the characteristic parameters of the tumor were obtained. At the same time, we punctured the related region and measured the HIF-1α, MVD and VEGF levels of the tissue biopsy site. The enhanced parameters and immunohistochemical factors were compared and studied.Results:The slope and Smax, SImax of the biopsy site was positively correlated with HIF-1α expression (γ were respectively 0.894, 0.832 and 0.681,P<0.05), and with the MVD (γ were respectively 0.629, 0.739 and 0.663,P<0.05). The slope and Smax, SImax of the biopsy site and VEGF expression was positively correlated (γ were respectively 0.876, 0.829 and 0.627,P<0.05); Even more, T1 onset, TTP was negatively correlated with HIF-1αexpression (γ were-0.692,-0.667,P<0.05 ), and with MVD (γ were-0.574,-0.835,P<0.05).T1 onset, TTP and VEGF expression was negatively correlated (γ were-0.907,-0.830,P<0.05).Conclusion:MRI biopsy site osteosarcoma dynamic enhancement parameters are statistically correlated with the expression of three factors and signiifcantly correlated with tissue hypoxia factor. Dynamic contrast-enhanced scans can provide a basis for the detection of hypoxia and angiogenesis in osteosarcoma tissue indirectly.