CAJ | 학술논문

对柔弱角毛藻 Chaetoceros debilis 和威氏海链藻 Thalassiosira weissflogii 分别喂食的安氏伪镖水蚤(Pseudodiaptomus annandalei)的核酸指标(包括RNA含量、DNA含量、RNA︰DNA、RNA︰protein和DNA︰protein)及元素组成[包括氮(N)含量、磷(P)含量、碳氮(C︰N)和碳磷(C︰P)比值]随个体发育的变化进行了描述，并对各阶段的生长率进行了测定。对RNA相关指标与生长率的相关性分析显示， RNA︰protein (RNA：PRO)与生长率不仅具有极类似的变化趋势，而且二者之间存在良好的相关性。这种正相关性同时适用于柔弱角毛藻喂食的所有发育阶段的蚤体和威氏海链藻喂食的桡足幼体和成体，因此， RNA︰PRO适合作为安氏伪镖水蚤生长的标志物。饵料转换实验中，经柔弱角毛藻喂食24h的桡足Ⅱ期幼体分别换作中肋骨条藻Skeletonema costatum和球形棕囊藻Phaeocystis globosa喂食或继续以柔弱角毛藻培养48 h后，蚤体的RNA含量和RNA︰PRO指标出现显著差异，呈现中肋骨条藻组>柔弱角毛藻组>球形棕囊藻组的趋势，而RNA︰DNA比值则是柔弱角毛藻=中肋骨条藻>球形棕囊藻。元素组成的差异主要体现在P含量和C︰N比值的变化上，中肋骨条藻和球形棕囊藻喂食的蚤体的各项指标并无明显差异，但均低于柔弱角毛藻喂食的蚤体。据此，推测 RNA 相关指标对饵料品质的变化响应较为迅速即时，而48h 内元素组成反映的主要是摄食历史。因此，现场调查中可利用 RNA︰PRO作为标志物迅速有效地指示桡足类的生长状态，而RNA相关的指标则均可用于桡足类营养状态的指示。
대유약각모조 Chaetoceros debilis 화위씨해련조 Thalassiosira weissflogii 분별위식적안씨위표수조(Pseudodiaptomus annandalei)적핵산지표(포괄RNA함량、DNA함량、RNA︰DNA、RNA︰protein화DNA︰protein)급원소조성[포괄담(N)함량、린(P)함량、탄담(C︰N)화탄린(C︰P)비치]수개체발육적변화진행료묘술，병대각계단적생장솔진행료측정。대RNA상관지표여생장솔적상관성분석현시， RNA︰protein (RNA：PRO)여생장솔불부구유겁유사적변화추세，이차이자지간존재량호적상관성。저충정상관성동시괄용우유약각모조위식적소유발육계단적조체화위씨해련조위식적뇨족유체화성체，인차， RNA︰PRO괄합작위안씨위표수조생장적표지물。이료전환실험중，경유약각모조위식24h적뇨족Ⅱ기유체분별환작중륵골조조Skeletonema costatum화구형종낭조Phaeocystis globosa위식혹계속이유약각모조배양48 h후，조체적RNA함량화RNA︰PRO지표출현현저차이，정현중륵골조조조>유약각모조조>구형종낭조조적추세，이RNA︰DNA비치칙시유약각모조=중륵골조조>구형종낭조。원소조성적차이주요체현재P함량화C︰N비치적변화상，중륵골조조화구형종낭조위식적조체적각항지표병무명현차이，단균저우유약각모조위식적조체。거차，추측 RNA 상관지표대이료품질적변화향응교위신속즉시，이48h 내원소조성반영적주요시섭식역사。인차，현장조사중가이용 RNA︰PRO작위표지물신속유효지지시뇨족류적생장상태，이RNA상관적지표칙균가용우뇨족류영양상태적지시。
Nauplii, copepodids and adults of Pseudodiaptomus annandalei were fed with Chaetoceros debilis and Thalassiosira weissflogii at the saturated food concentration. Biochemical indices were then measured and described including mass specific RNA content, DNA conten, RNA︰DNA, RNA︰protein, and DNA︰protein, as well as the elemental compositions (mass specific phosphorus content, nitrogen content, atomic C︰P, and C︰N ratios). Their growth rates were also determined for different developmental stages. The relationships between growth rate and three RNA-related indices were analyzed. It was shown that RNA︰protein (RNA︰PRO), varying with developmental stage in the same trend as growth rate, was correlated with growth rate significantly in a positive linear manner for all life stages of P. annandalei on C. debilis and for older stages than nauplii on T. weissflogii. Thus, RNA︰ PRO could be applied as an indicator of P. annandalei growth. In the experiment of changing diet, the CII copepodids with a 24 h feeding history on C. debilis continued to be fed with Skeletonema costatum or Phaeoecystis globosa for another 48 h, and then showed remarkable differences in RNA content and RNA︰PRO ratio, which ranked as S. costatum > C. debilis> P. globosa treatment. Differently, RNA︰DNA ratios ranked as S. costatum =C. debilis>P. globosa treatment. On the other hand, elemental compositions of those copepodids in different treatments differed in phosphorus content and atomic C︰N ratio, which ranked as C. debilis >S. costatum = P. globosa. It can be deduced that RNA-related indices can respond immediately to food change within 48 h while elemental compositions only reflect feeding history. It was concluded that RNA︰PRO can be applied in field investigation as an indicator of growth in copepod, and RNA-related indices can be used to indicate nutrition status.