CAJ | 학술논문

目的：建立复方醋酸氟轻松乳膏中尿囊素和冰片的定量方法。方法尿囊素采用Intersil ODS-3 C18柱（250mm×4.6mm，5μm），流动相为甲醇：水（2：98），检测波长为210nm，流速为1.0mL/min，外标法计算。冰片以水杨酸甲酯为内标物，采用气相色谱法，DB-WAX毛细管色谱柱（30m×0.53mm×1.0μm），柱温130℃，进样口温度220℃，FID检测器，检测器温度为280℃。结果尿囊素和冰片的线性范围分别为0.05015～1.003 mg/mL和0.4728～4.728mg/mL，回归方程分别为Y=130310X+1418.5（r=0.9998）和Y=1.2213X-0.0082（r=1.0000）。尿囊素和冰片的平均回收率分别为102.12％（RSD=0.59％）和100.25％（RSD=0.60％）。结论本方法简便、快捷，灵敏度高，结果准确，可用于本制剂的质量控制。
목적：건립복방작산불경송유고중뇨낭소화빙편적정량방법。방법뇨낭소채용Intersil ODS-3 C18주（250mm×4.6mm，5μm），류동상위갑순：수（2：98），검측파장위210nm，류속위1.0mL/min，외표법계산。빙편이수양산갑지위내표물，채용기상색보법，DB-WAX모세관색보주（30m×0.53mm×1.0μm），주온130℃，진양구온도220℃，FID검측기，검측기온도위280℃。결과뇨낭소화빙편적선성범위분별위0.05015～1.003 mg/mL화0.4728～4.728mg/mL，회귀방정분별위Y=130310X+1418.5（r=0.9998）화Y=1.2213X-0.0082（r=1.0000）。뇨낭소화빙편적평균회수솔분별위102.12％（RSD=0.59％）화100.25％（RSD=0.60％）。결론본방법간편、쾌첩，령민도고，결과준학，가용우본제제적질량공제。
Objective To establish a method for the determination of allantoin and borneol in compound fluocinonide cream.Methods Allantoin was detected by Intersil ODS-3 C18 column(250mm×4.6mm, 5μm) with methanol-water(2:98) as the mobile phase at the flow rate of 1.0mL/min. The detection wavelength was at 210 nm. It was calculated by the external standard method. Borneol. Methyl salicylate was selected as internal standard. Gas chromatography was adopted. The column was DB-WAX capillary column (30m×0.53mm×1.0μm) and column temperature was 130℃. The injector temperature was 220℃ and the detector was FID whose temperature was 280℃. Results The linear range of allantoin and borneol were 0.05015 to 1.003mg/mL and 0.4728 to 4.728mg/mL. Regression equations wereY=130310X+1418.5(r=0.9998) andY=1.2213X-0.0082(r=1.0000) respectively. The average recovery of allantoin and borneol were 102.12%(RSD=0.59%)and 100.25%(RSD=0.60%)respectively. Conclusion The method is simple, rapid, sensitive, accurate and reliable which can be used for quality control of compound fluocinonide cream.