甘肃农业科技
甘肅農業科技
감숙농업과기
GANSU AGRICULTURAL SCIENCE AND TECHNOLOGY
2015年
8期
25-28
,共4页
马铃薯%DNA提取%改良CTAB%试剂盒
馬鈴藷%DNA提取%改良CTAB%試劑盒
마령서%DNA제취%개량CTAB%시제합
Potato%DNA Extraction%Improved CTAB%Reagent box
以马铃薯叶片为试验材料，采用改良CTAB法和试剂盒法提取马铃薯叶片基因组DNA。结果表明，2种方法均可得到满足SSR-PCR扩增要求的DNA，其中试剂盒较改良CTAB法提取的DNA质量高、杂质少，DNA得率低，操作简单，耗时短，毒害小，但费用较改良CTAB法高。实践中可根据条件选择适宜方法提取马铃薯基因组DNA。
以馬鈴藷葉片為試驗材料，採用改良CTAB法和試劑盒法提取馬鈴藷葉片基因組DNA。結果錶明，2種方法均可得到滿足SSR-PCR擴增要求的DNA，其中試劑盒較改良CTAB法提取的DNA質量高、雜質少，DNA得率低，操作簡單，耗時短，毒害小，但費用較改良CTAB法高。實踐中可根據條件選擇適宜方法提取馬鈴藷基因組DNA。
이마령서협편위시험재료，채용개량CTAB법화시제합법제취마령서협편기인조DNA。결과표명，2충방법균가득도만족SSR-PCR확증요구적DNA，기중시제합교개량CTAB법제취적DNA질량고、잡질소，DNA득솔저，조작간단，모시단，독해소，단비용교개량CTAB법고。실천중가근거조건선택괄의방법제취마령서기인조DNA。
The potato leaves as test materials,improved CTAB method and plant genomic DNA extraction Kit is used to extract genomic DNA from leaves of potato in field respectively. The result shows that DNA extacted by the two methods also met demands for SSR-PCR amplification;The quality of DNA obtained by plant genomic DNA Kit is better but the achievement rate of DNA is lower. Moreover,the method of plant genomic DNA Kit is much easier,needed shorter time,had lower toxicity,and high cost than improved CATB method. Suitable method can be choose to genomic DNA of potato according to the actual conditions of the laboratory.
