当代医学
噹代醫學
당대의학
CHINA CONTEMPORARY MEDICINE
2015年
23期
15-16
,共2页
丙泊酚%肝缺血再灌注%肾损伤
丙泊酚%肝缺血再灌註%腎損傷
병박분%간결혈재관주%신손상
Propofol%Hepatic ischemia-reperfusion%Renal injury
目的 评价丙泊酚后处理对大鼠肝缺血再灌注时远隔脏器肾脏细胞B淋巴细胞瘤/白血病-2(Bcl-2),Bcl相关X蛋白(Bax)的影响.方法 健康雄性SD大鼠54只,随机分为3组(n=18):假手术(S)组、缺血再灌注(IR)组、丙泊酚(P)组.每组再根据再灌注时间(2、4、6h)的不同各分成3个亚组,分别为S 2、S 4、S 6、IR 2、IR 4、IR 6、P 2、P 4、P 6亚组.制备70%肝缺血再灌注模型,测定血液中胱抑素C(Cys-c),利用免疫组化方法检测各组肾组织中Bcl-2蛋白和Bax的表达,TUNEL法检测肾脏细胞的凋亡指数(AI).结果 各组同一时间点相比,IR组和P组的Cys-c、Bcl-2、Bax蛋白含量,AI值均显著高于S组(P<0.05).P组的Bcl-2、Bcl-2/Bax显著高于IR组,Bax、Cys-c、AI值显著低于IR组(P<0.05).结论 丙泊酚对肝缺血再灌注时肾脏损伤有保护作用,而其保护机制可能与调控Bcl-2、Bax蛋白的表达有关.
目的 評價丙泊酚後處理對大鼠肝缺血再灌註時遠隔髒器腎髒細胞B淋巴細胞瘤/白血病-2(Bcl-2),Bcl相關X蛋白(Bax)的影響.方法 健康雄性SD大鼠54隻,隨機分為3組(n=18):假手術(S)組、缺血再灌註(IR)組、丙泊酚(P)組.每組再根據再灌註時間(2、4、6h)的不同各分成3箇亞組,分彆為S 2、S 4、S 6、IR 2、IR 4、IR 6、P 2、P 4、P 6亞組.製備70%肝缺血再灌註模型,測定血液中胱抑素C(Cys-c),利用免疫組化方法檢測各組腎組織中Bcl-2蛋白和Bax的錶達,TUNEL法檢測腎髒細胞的凋亡指數(AI).結果 各組同一時間點相比,IR組和P組的Cys-c、Bcl-2、Bax蛋白含量,AI值均顯著高于S組(P<0.05).P組的Bcl-2、Bcl-2/Bax顯著高于IR組,Bax、Cys-c、AI值顯著低于IR組(P<0.05).結論 丙泊酚對肝缺血再灌註時腎髒損傷有保護作用,而其保護機製可能與調控Bcl-2、Bax蛋白的錶達有關.
목적 평개병박분후처리대대서간결혈재관주시원격장기신장세포B림파세포류/백혈병-2(Bcl-2),Bcl상관X단백(Bax)적영향.방법 건강웅성SD대서54지,수궤분위3조(n=18):가수술(S)조、결혈재관주(IR)조、병박분(P)조.매조재근거재관주시간(2、4、6h)적불동각분성3개아조,분별위S 2、S 4、S 6、IR 2、IR 4、IR 6、P 2、P 4、P 6아조.제비70%간결혈재관주모형,측정혈액중광억소C(Cys-c),이용면역조화방법검측각조신조직중Bcl-2단백화Bax적표체,TUNEL법검측신장세포적조망지수(AI).결과 각조동일시간점상비,IR조화P조적Cys-c、Bcl-2、Bax단백함량,AI치균현저고우S조(P<0.05).P조적Bcl-2、Bcl-2/Bax현저고우IR조,Bax、Cys-c、AI치현저저우IR조(P<0.05).결론 병박분대간결혈재관주시신장손상유보호작용,이기보호궤제가능여조공Bcl-2、Bax단백적표체유관.
Objective To investigate the effect of post-conditioning with Propofol on apoptosis and the expression of bcl-2,bax gene in renal tissue after hepatic ischemia-reperfusion injury and its mechanism.Methods Fifty-four healthy male SD rats were randomly divided into three groups,including sham group (S),ischemia-reperfusion group (IR),propofol group (P).According to the different periods after reperfusion,each group was further divided into 2-hour 4-hour and 6-hour reperfusion subgroups respectively (n=6 in each subgroup),named S2、S4、S6 subgroups,andI R2、IR4、IR6 subgroups,and P2、P4、P6 subgroups.Make the 70% hepatic ischemia-reperfusion model.The blood was sampled at the end of ischemia-reperfusion for assay of cystatin c(Cys-c)the apoptosis rate(AI) in ischemia-reperfusion renal tissure were measured by TUNEL method; the bcl-2,bax concentration were measured by immuno-histochemistry.Results Different group compared to the sametime.Blood concentrations of Cys-c,AI,and the amount of bcl-2 and bax protein after reperfusion in group IR,and group P were higher than those in group S at matched reperfusion time points (P<0.05).The amount of bcl-2 and bcl-2/bax in group P were higher than those in group IR,but the amount of bax,AI and the concentrations of Cys-c in group P were lower than those in group IR (P<0.05).Conclusion Propofol can alleviate renal injury induced by hepatic ischemia-reperfusion in rats,in which the bcl-2 and bax protein may play important roles.
