CAJ | 학술논문

目的：观察消痰散结方对MKN-45人胃癌裸鼠原位移植瘤细胞超微结构的影响,探讨其治疗胃癌的作用机制。方法采用MKN-45人胃癌细胞株建立裸鼠皮下瘤模型皮下传3代作为实验模型的瘤源,采用0B胶黏合法建立人胃癌裸鼠原位移植瘤模型。40只模型裸鼠分为空白对照组、模型组、消痰散结方组、替加氟组,每组10只。消痰散结方组给予消痰散结方灌胃,替加氟组给予替加氟灌胃,模型组给予生理盐水灌胃,空白对照组正常饮食喂养。干预6周后,测瘤质量,计算抑瘤率。透射电镜观察药物作用后移植瘤细胞超微结构。结果消痰散结方组瘤质量明显低于空白对照组及模型组(P＜0.05,P＜0.01),其抑瘤率为46.2%；电镜观察可见胃癌细胞出现典型细胞凋亡的形态学改变。结论消痰散结方具有诱导MKN-45人胃癌裸鼠原位移植瘤细胞凋亡的作用,提示诱导细胞凋亡是消痰散结方治疗胃癌的可能作用机制之一。
목적：관찰소담산결방대MKN-45인위암라서원위이식류세포초미결구적영향,탐토기치료위암적작용궤제。방법채용MKN-45인위암세포주건립라서피하류모형피하전3대작위실험모형적류원,채용0B효점합법건립인위암라서원위이식류모형。40지모형라서분위공백대조조、모형조、소담산결방조、체가불조,매조10지。소담산결방조급여소담산결방관위,체가불조급여체가불관위,모형조급여생리염수관위,공백대조조정상음식위양。간예6주후,측류질량,계산억류솔。투사전경관찰약물작용후이식류세포초미결구。결과소담산결방조류질량명현저우공백대조조급모형조(P＜0.05,P＜0.01),기억류솔위46.2%；전경관찰가견위암세포출현전형세포조망적형태학개변。결론소담산결방구유유도MKN-45인위암라서원위이식류세포조망적작용,제시유도세포조망시소담산결방치료위암적가능작용궤제지일。
Objective To observe the effects ofXiaotan Sanjie Decoction on ultramicrostructure in MKN-45 human gastric cancer orthotopic xenograft;To discuss its mechanism for gastric cancer. Methods MKN-45 human gastric cancer cell line was used to establish subcutaneous tumor model in nude mice as an experimental model which passed three generations of tumor source. Then OB glue was used to establish human gastric cancer orthotopic xenograft model. 40 model nude mice were divided into control group, model group (received gavage with saline),Xiaotan Sanjie Decoction group (received garage withXiaotan Sanjie Decoction) and Tegafur group (received gavage with Tegafur), 10 mice in each group. Mice in the control group were under normal diet, without medicine intervention. After 6 weeks of treatment intervention, tumor weight was detected, inhibitory rate was calculated, the ultrastructural changes of gastric cancer cells were detected through transmission electron microscopy.Results The tumor weight inXiaotan Sanjie Decoction group was significantly lower than that in control group and model group (P<0.05,P<0.01). The tumor inhibition rate was 46.2%. Under the electron microscope, MKN-45 gastric cancer cells strain changed to typical apoptosis.ConclusionXiaotan Sanjie Decoction can lead to apoptosis effects on MKN-45 human gastric cancer orthotopic xenografts. All evidences indicate that inducing apoptosis may be one of the most important mechanisms ofXiaotan Sanjie Decoction in treating gastric cancer.