CAJ | 학술논문

目的：探讨手术方法建立心肌缺血型冠心病与肾虚血瘀病证结合模型的可行性。方法采取惊吓、置于寒冷环境并皮下注射氢化可的松的方法制备非疾病肾虚血瘀证候模型,结扎冠状动脉前降支并注射氢化可的松建立心肌缺血型冠心病肾虚血瘀病证结合模型。实验大鼠分为肾虚血瘀组、病证结合组和正常组,每组5只。观察大鼠造模前后体温、体质量、呼吸频率、心率、全血黏度、卡松黏度。依据中医临床肾虚血瘀证诊断标准,对2种模型中医证候进行比较。结果与正常组和本组造模前比较,肾虚血瘀组、病证结合组大鼠造模后均出现体温下降、呼吸频率增快(P＜0.05)；与正常组比较,病证结合组、肾虚血瘀组体质量减轻或者增长缓慢、全血黏度及卡松黏度升高(P＜0.05,P＜0.01)；造模两组间全血黏度、心电图比较差异有统计学意义(P＜0.05)。结论2种模型在中医证候方面无明显差异,符合中医临床肾虚血瘀证的诊断标准。
목적：탐토수술방법건립심기결혈형관심병여신허혈어병증결합모형적가행성。방법채취량하、치우한랭배경병피하주사경화가적송적방법제비비질병신허혈어증후모형,결찰관상동맥전강지병주사경화가적송건립심기결혈형관심병신허혈어병증결합모형。실험대서분위신허혈어조、병증결합조화정상조,매조5지。관찰대서조모전후체온、체질량、호흡빈솔、심솔、전혈점도、잡송점도。의거중의림상신허혈어증진단표준,대2충모형중의증후진행비교。결과여정상조화본조조모전비교,신허혈어조、병증결합조대서조모후균출현체온하강、호흡빈솔증쾌(P＜0.05)；여정상조비교,병증결합조、신허혈어조체질량감경혹자증장완만、전혈점도급잡송점도승고(P＜0.05,P＜0.01)；조모량조간전혈점도、심전도비교차이유통계학의의(P＜0.05)。결론2충모형재중의증후방면무명현차이,부합중의림상신허혈어증적진단표준。
Objective To discuss the feasibility of establishing the model of combining myocardial ischemic coronary disease with syndrome of kidney deficiency and blood stasis by surgical method.Methods Non-disease of kidney deficiency and blood stasis syndrome model was built by taking the methods of fright, being placed in a cold environment and injection of hydrocortisone. The model of combining the myocardial ischemic coronary disease with the syndrome of kidney deficiency and blood stasis was established through ligating the left anterior descending branch of artery and injecting hydrocortisone. The rats were divided into kidney deficiency and blood stasis group, combination of disease and syndrome group, and normal group, 5 rats in each group. The temperature, weight, heart rate, breathing rate and whole blood viscosity, casson viscosity of the rats in the two groups before and after modeling were observed. According to TCM clinical diagnosis criteria of kidney deficiency and blood stasis syndrome, TCM syndrome characteristics of the two groups were compared.ResultsCompared with normal groups and before modeling, rat temperature dropped and breathing rate increased in kidney deficiency and blood stasis group, combination of disease and syndrome group (P<0.05). Compared with normal group, rat weight decreased or grew slowly, and whole blood viscosity and casson viscosity increased (P<0.05, P<0.01). There were statistical significant differences in whole blood viscosity and electrocardiogram between the two groups (P<0.05).Conclusion There is no obvious difference between TCM syndrome characteristics of the two groups. They all meet the TCM clinical diagnosis criteria of kidney deficiency and blood stasis syndrome.