CAJ | 학술논문

目的：探讨肾虚质与脑功能衰退的相关性及肾藏志理论的物质基础与神经生物学机制。方法采用“猫吓鼠”造模法造成先天不足加后天失养复合型肾虚模型,随机分为模型组、左归丸组和右归丸组；空白组产自正常孕鼠。对仔鼠恐吓同时开始灌胃,左归丸组、右归丸组分别给予左归丸、右归丸混悬液,空白组和模型组给予等量生理盐水,1次/d,连续2个月。悬吊试验和Y迷宫实验观测大鼠运动和空间学习记忆能力,免疫组化法检测N-甲基-D-天冬氨酸(NMDA)受体亚基NR2A、NR2B蛋白表达。结果悬吊试验：模型组持续时间低于空白组(P＜0.05),左归丸组和右归丸组高于模型组(P＜0.05)；Y迷宫实验：模型组跑至安全区正确次数低于空白组(P＜0.05),左归丸组和右归丸组高于模型组(P＜0.05)；模型组 NR2A、NR2B 光密度值较空白组降低(P＜0.05),左归丸组和右归丸组光密度值较模型组升高(P＜0.05)。结论肾虚质大鼠神经行为学异常且海马NMDA受体亚基表达降低；左归丸、右归丸可上调NMDA受体表达且改善大鼠脑功能,揭示了肾虚质与脑功能衰退的相关性及肾藏志理论的物质基础与神经生物学机制。
목적：탐토신허질여뇌공능쇠퇴적상관성급신장지이론적물질기출여신경생물학궤제。방법채용“묘하서”조모법조성선천불족가후천실양복합형신허모형,수궤분위모형조、좌귀환조화우귀환조；공백조산자정상잉서。대자서공하동시개시관위,좌귀환조、우귀환조분별급여좌귀환、우귀환혼현액,공백조화모형조급여등량생리염수,1차/d,련속2개월。현조시험화Y미궁실험관측대서운동화공간학습기억능력,면역조화법검측N-갑기-D-천동안산(NMDA)수체아기NR2A、NR2B단백표체。결과현조시험：모형조지속시간저우공백조(P＜0.05),좌귀환조화우귀환조고우모형조(P＜0.05)；Y미궁실험：모형조포지안전구정학차수저우공백조(P＜0.05),좌귀환조화우귀환조고우모형조(P＜0.05)；모형조 NR2A、NR2B 광밀도치교공백조강저(P＜0.05),좌귀환조화우귀환조광밀도치교모형조승고(P＜0.05)。결론신허질대서신경행위학이상차해마NMDA수체아기표체강저；좌귀환、우귀환가상조NMDA수체표체차개선대서뇌공능,게시료신허질여뇌공능쇠퇴적상관성급신장지이론적물질기출여신경생물학궤제。
ObjectiveTo discuss the correlation between kidney-deficiency constitution and brain function decline, and material basis of kidney-storing-spirit theory and neurobiology mechanism. Methods By employing the method of “cats scare rats”, composite offspring rat models with deficiency and acquired dystrophy were built, then they were divided into model group,ZuoguiPill group and Yougui Pill group. Blank group was composed from normal pregnant rats. When the intimidation of offspring began,Zuogui Pill andYougui Pill groups received gavage with corresponding doses of medicine, 1 times per day for 2 consecutive months. Model and blank groups received the same amount of normal saline. Exercise capacity was detected by suspension test. Learning and memory capacity was detected by Y maze test. Expressions of NMDA receptor subunits NR2A and NR2B were detected by immunohistochemical method.Results In suspension test, the duration of model group was shorter than blank group (P<0.05), while duration ofZuogui Pill andYougui Pill groups was longer than the model group (P<0.05). In Y maze test, the correct number of model group was less than blank group, and increased significantly after treatment (P<0.05). The optical density of NR2A and NR2B was lower in model group than blank group (P<0.05) and higher inZuogui Pill andYougui Pill groups than model group (P<0.05).ConclusionThe neurobehavioral is abnormal and NMDA receptor expression depresses in rats with kidney- deficiency constitution.ZuoguiPill andYouguiPill can rise NMDA receptor expression and improve brain function of rats, which reveal the correlation between kidney-deficiency constitution and brain function decline, and material basis of kidney-storing-spirit theory and neurobiology mechanism.