CAJ | 학술논문

目的：观察补阳还五精简方对局灶性脑缺血大鼠脑组织APJ蛋白和基因表达的影响，并探讨其作用机制。方法采用大脑中动脉线栓法建立大鼠局灶性脑缺血模型，实验大鼠随机分为假手术组、模型组、补阳还五汤组和补阳还五精简方组，各给药组给予相应药物灌胃。分别于给药第3、7日Western blot、RT-PCR检测各组大鼠脑组织APJ蛋白和基因表达。结果补阳还五精简方组和补阳还五汤组各时间点APJ蛋白和基因表达均高于模型组和假手术组(P＜0.05)，补阳还五精简方组与补阳还五汤组各时间点APJ蛋白表达无明显差异，但补阳还五精简方组APJ基因表达高于补阳还五汤组(P＜0.05)。结论补阳还五精简方可通过促进缺血后脑组织APJ的表达发挥对大鼠脑组织的神经保护和修复作用。
목적：관찰보양환오정간방대국조성뇌결혈대서뇌조직APJ단백화기인표체적영향，병탐토기작용궤제。방법채용대뇌중동맥선전법건립대서국조성뇌결혈모형，실험대서수궤분위가수술조、모형조、보양환오탕조화보양환오정간방조，각급약조급여상응약물관위。분별우급약제3、7일Western blot、RT-PCR검측각조대서뇌조직APJ단백화기인표체。결과보양환오정간방조화보양환오탕조각시간점APJ단백화기인표체균고우모형조화가수술조(P＜0.05)，보양환오정간방조여보양환오탕조각시간점APJ단백표체무명현차이，단보양환오정간방조APJ기인표체고우보양환오탕조(P＜0.05)。결론보양환오정간방가통과촉진결혈후뇌조직APJ적표체발휘대대서뇌조직적신경보호화수복작용。
Objective To observe the effects of simplified recipe ofBuyang Huanwu Decoction on expression of APJ in the brain of local cerebral ischemia rats;To discuss its mechanism of action. MethodsFocal cerebral ischemia rat models were established by middle cerebral arterial occlusion. The adult rats were randomly divided into sham-operation group, model group,Buyang Huanwu Decoction group and simplified recipe ofBuyang Huanwu Decoction group. Administration groups were given relevant medicine for gavage. The expressions of APJ protein and APJ mRNA at different time points were detected by Western blot and RT-PCR.ResultsCompared with model group and sham-operation group, the expression of APJ protein and APJ mRNA at different time points in Buyang Huanwu Decoction group and simplified recipe ofBuyang Huanwu Decoction group significantly increased (P<0.05). The expression of APJ protein at different time points showed no difference between simplified recipe ofBuyang Huanwu Decoction group andBuyang Huanwu Decoction group;while the expression of APJ mRNA in simplified recipe ofBuyang Huanwu Decoction group was higher than Buyang Huanwu Decoction group (P<0.05).ConclusionSimplified recipe ofBuyang Huanwu Decoction plays a role in neural protection and restoration by promoting the expression of APJ in the brain of focal cerebral ischemia rats.