CAJ | 학술논문

建立基于悬浮固化液相微萃取预处理的高效液相色谱－荧光检测法（ SFODME－HPLC－FLD）用于两种植物生长素（吲哚丙酸和吲哚丁酸）的分析．实验考察各因素对萃取过程的影响，确定最佳条件：50μL十二醇（纯）为萃取剂，20．0 mL样品溶液（pH 3．0）、0．25 g· mL－1 NaCl、26℃、1800 r· min－1条件下萃取10 min．结果表明， SFODME技术重现性好、灵敏度高，相对标准偏差小于8．66％，检测限为0．01 ng· mL－1，线性范围为0．02～60 ng· mL－1，加标回收率大于84．3％．方法快速、稳定、环境友好，适用于实际样品的检测．
건립기우현부고화액상미췌취예처리적고효액상색보－형광검측법（ SFODME－HPLC－FLD）용우량충식물생장소（신타병산화신타정산）적분석．실험고찰각인소대췌취과정적영향，학정최가조건：50μL십이순（순）위췌취제，20．0 mL양품용액（pH 3．0）、0．25 g· mL－1 NaCl、26℃、1800 r· min－1조건하췌취10 min．결과표명， SFODME기술중현성호、령민도고，상대표준편차소우8．66％，검측한위0．01 ng· mL－1，선성범위위0．02～60 ng· mL－1，가표회수솔대우84．3％．방법쾌속、은정、배경우호，괄용우실제양품적검측．
Determination of two anxins ( indole propionic acid and indole butyric acid) by solidifica-tion of floated organic drop microextraction with high -performance liquid chromatography-fluores-cence detection ( SFODME-HPLC-FLD) was established.The experimental conditions affecting the extraction process were optimized.The optimal conditions of SFODME were as follows:50μL dode-canol as extraction solvent, 20.0 mL sample solution with pH 3.0 and 0.25 g · mL-1 NaCl.The above solution was extracted for 10 min at temperature of 26 ℃ and centrifugation speed of 1 800 r· min-1 .Results showed that this SFODME approach had high sensitivity and good reproducibility, with relative standard deviations lower than 8.66%.Its linear range was 0.02~60 ng· mL-1 , with the limit of detection of 0.01 ng · mL-1 and the recoveries larger than 84.3%.This method was rapid, stable and environmentally benign, and it can be applied to real sample analysis.