CAJ | 학술논문

目的：研究杂合病毒介导的IL-24基因对口腔鳞状细胞癌耐药细胞的凋亡诱导作用，探讨其治疗耐药口腔癌的可能性。方法：通过MTT细胞活力测定鉴定口腔鳞状细胞癌细胞株KB和其耐药细胞株KBV。利用杂合病毒AdLTR2EF1α搭载EGFP和IL-24转染KB细胞，确定最佳转染浓度，荧光倒置显微镜及RT-PCR鉴定EGFP或IL-24基因表达。以人永生化上皮细胞HaCaT细胞作为对照，使用AdLTR2EF1α-IL-24转染KB、KBV及HaCaT细胞后， MMT法检测细胞活力， AnnexinV/PI双染法检测细胞凋亡，透射电镜观察细胞凋亡形态。结果：KBV细胞对长春新碱的耐受性明显高于KB细胞。 AdLTR2EF1α-IL-24搭载的IL-24基因可以在KB及KBV细胞内过表达。 IL-24基因在KB和KBV细胞中的过表达，有明显抑制细胞生长和促凋亡作用，而在HaCaT细胞中没有类似的作用。结论：杂合病毒介导的IL-24对口腔鳞状细胞癌耐药细胞有较强的细胞毒性和诱导凋亡作用，并且对正常组织细胞没有影响。具有治疗耐药口腔癌的潜在价值。
목적：연구잡합병독개도적IL-24기인대구강린상세포암내약세포적조망유도작용，탐토기치료내약구강암적가능성。방법：통과MTT세포활력측정감정구강린상세포암세포주KB화기내약세포주KBV。이용잡합병독AdLTR2EF1α탑재EGFP화IL-24전염KB세포，학정최가전염농도，형광도치현미경급RT-PCR감정EGFP혹IL-24기인표체。이인영생화상피세포HaCaT세포작위대조，사용AdLTR2EF1α-IL-24전염KB、KBV급HaCaT세포후， MMT법검측세포활력， AnnexinV/PI쌍염법검측세포조망，투사전경관찰세포조망형태。결과：KBV세포대장춘신감적내수성명현고우KB세포。 AdLTR2EF1α-IL-24탑재적IL-24기인가이재KB급KBV세포내과표체。 IL-24기인재KB화KBV세포중적과표체，유명현억제세포생장화촉조망작용，이재HaCaT세포중몰유유사적작용。결론：잡합병독개도적IL-24대구강린상세포암내약세포유교강적세포독성화유도조망작용，병차대정상조직세포몰유영향。구유치료내약구강암적잠재개치。
Objective:To study the apoptosis-inducing effect of hybrid virus vector-mediated IL-24 gene on oral squamous cell carcinoma (OSCC) cell line and drug-resistant cell line. Methods:In order to identify OSCC cell line KB and the drug resistant counterparts cell lines KBV, cell viability was measured by MTT assay. Fluorescence microscopy and RT-PCR experiments were used to detect the gene expression, AdLTR2EF1α-EGFP or AdLTR2EF1α-IL-24 were used to treat the KB cells in order to obtain the optimal drug concentration. Human keratinocyte HaCat cells were used as control cells. AdLTR2EF1α-IL-24 and AdLTR2EF1α-vec transfected the KB ,KBV, and HaCat cells in a 200 μM substrate solution, and incubated at 37℃ for 1, 3, 5 and 7days respectively. Formation of cells in the sub-G1 phase was indicative of apoptotic cells. Cell viability and apoptosis rate were measured by the MTT assay, flow cytometry. Cells morphology were observed by transmission electron microscope. Results: Vincristine resistance in KBV cells was significantly higher than KB cells. The expression of IL-24 was detected in both transfected KB cells and KBV cells. IL-24 could inhibit the growth of KB cells and KBV cells, while no growth inhibition in HaCaT cells. Overexpression of IL-24 could induce apoptosis in KB and KBV cells, but there was no similar effect in HaCaT cells. Conclusion:Hybrid virus-mediated IL-24 had strong cytotoxic-ity and induction apoptosis effects on OSCC drug-resistant KBV cell line, and had no side effects on normal cells, which may develop a new way to treat oral drug resistant cancer.