中华肝脏外科手术学电子杂志
中華肝髒外科手術學電子雜誌
중화간장외과수술학전자잡지
CHINESE JOURNAL OF HEPATIC SURGERY(ELECTRONIC EDITION)
2015年
4期
254-258
,共5页
谢汉镔%葛缅%池信锦%罗刚健%黑子清%陆慧琼
謝漢鑌%葛緬%池信錦%囉剛健%黑子清%陸慧瓊
사한빈%갈면%지신금%라강건%흑자청%륙혜경
肝移植%胃疾病%抗氧化剂%硫氧还蛋白%活性氧%大鼠
肝移植%胃疾病%抗氧化劑%硫氧還蛋白%活性氧%大鼠
간이식%위질병%항양화제%류양환단백%활성양%대서
Liver transplantation%Stomach disease%Antioxidant%Thioredoxin%Reactive oxygen species%Rat
目的：探讨肝移植围手术期缺血-再灌注胃氧化损伤发生的规律及机制。方法将28只SD大鼠按随机数字表法分为4组，每组7只。其中3组建立自体原位肝移植模型。根据移植肝恢复灌注时间设立4、8、16 h组。另1组设为假手术组（Sham组）。取大鼠胃壁组织HE染色后观察胃黏膜损伤的病理学变化。检测胃组织超氧阴离子（O2-）、丙二醛（MDA）、谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH-PX）水平。采用Western blot法检测胃组织硫氧还蛋白（Trx）-2表达。实验数据比较采用单因素方差分析及LSD-t检验。结果4 h组胃黏膜深层及黏膜下层充血、水肿、出血，腺体排列紊乱，部分区域出血、坏死，糜烂深达黏膜肌层；8、16 h组胃黏膜损伤减轻，仅黏膜浅层和深层充血、水肿，Sham组表现为多数胃黏膜上皮结构完整。4 h组O2-和MDA分别为（185±26） U/mg和（0.4±0.1） nmol/mg，8 h组相应为（192±59）U/mg和（0.5±0.1）nmol/mg，明显高于Sham组的（102±34）U/mg和（0.2±0.1）nmol/mg（LSD-t=4.99，4.23和6.37，4.52；P<0.05）。4 h组GSH和GSH-PX分别为（17±6）mg/g和（781±174）U/mg，8 h组相应为（15±4）mg/g和（750±160）U/mg，明显低于Sham组的（30±6）mg/g和（1162±215）U/mg（LSD-t=-3.26，-4.01和-3.78，-4.36；P<0.05）。16 h组大鼠胃组织O2-、MDA、GSH、GSH-PX分别为（169±27）U/mg、（0.3±0.1）nmol/mg、（25±8）mg/g、（1108±183）U/mg，与8 h组比较差异有统计学意义（LSD-t=-2.85，-3.46，2.66，3.69；P<0.05）。4、8 h组Trx-2蛋白相对表达量分别为52±10、43±8，较Sham组的125±16明显减少（LSD-t=-5.34，-6.23；P<0.05）。16 h组Trx-2蛋白表达量为160±18，较8 h组明显升高（LSD-t=4.75， P<0.05）。结论大鼠肝移植缺血-再灌注损伤早期即引起胃氧化损伤，其发生可能与Trx-2表达下降、活性氧增多和机体抗氧化能力下降有关。
目的：探討肝移植圍手術期缺血-再灌註胃氧化損傷髮生的規律及機製。方法將28隻SD大鼠按隨機數字錶法分為4組，每組7隻。其中3組建立自體原位肝移植模型。根據移植肝恢複灌註時間設立4、8、16 h組。另1組設為假手術組（Sham組）。取大鼠胃壁組織HE染色後觀察胃黏膜損傷的病理學變化。檢測胃組織超氧陰離子（O2-）、丙二醛（MDA）、穀胱甘肽（GSH）、穀胱甘肽過氧化物酶（GSH-PX）水平。採用Western blot法檢測胃組織硫氧還蛋白（Trx）-2錶達。實驗數據比較採用單因素方差分析及LSD-t檢驗。結果4 h組胃黏膜深層及黏膜下層充血、水腫、齣血，腺體排列紊亂，部分區域齣血、壞死，糜爛深達黏膜肌層；8、16 h組胃黏膜損傷減輕，僅黏膜淺層和深層充血、水腫，Sham組錶現為多數胃黏膜上皮結構完整。4 h組O2-和MDA分彆為（185±26） U/mg和（0.4±0.1） nmol/mg，8 h組相應為（192±59）U/mg和（0.5±0.1）nmol/mg，明顯高于Sham組的（102±34）U/mg和（0.2±0.1）nmol/mg（LSD-t=4.99，4.23和6.37，4.52；P<0.05）。4 h組GSH和GSH-PX分彆為（17±6）mg/g和（781±174）U/mg，8 h組相應為（15±4）mg/g和（750±160）U/mg，明顯低于Sham組的（30±6）mg/g和（1162±215）U/mg（LSD-t=-3.26，-4.01和-3.78，-4.36；P<0.05）。16 h組大鼠胃組織O2-、MDA、GSH、GSH-PX分彆為（169±27）U/mg、（0.3±0.1）nmol/mg、（25±8）mg/g、（1108±183）U/mg，與8 h組比較差異有統計學意義（LSD-t=-2.85，-3.46，2.66，3.69；P<0.05）。4、8 h組Trx-2蛋白相對錶達量分彆為52±10、43±8，較Sham組的125±16明顯減少（LSD-t=-5.34，-6.23；P<0.05）。16 h組Trx-2蛋白錶達量為160±18，較8 h組明顯升高（LSD-t=4.75， P<0.05）。結論大鼠肝移植缺血-再灌註損傷早期即引起胃氧化損傷，其髮生可能與Trx-2錶達下降、活性氧增多和機體抗氧化能力下降有關。
목적：탐토간이식위수술기결혈-재관주위양화손상발생적규률급궤제。방법장28지SD대서안수궤수자표법분위4조，매조7지。기중3조건립자체원위간이식모형。근거이식간회복관주시간설립4、8、16 h조。령1조설위가수술조（Sham조）。취대서위벽조직HE염색후관찰위점막손상적병이학변화。검측위조직초양음리자（O2-）、병이철（MDA）、곡광감태（GSH）、곡광감태과양화물매（GSH-PX）수평。채용Western blot법검측위조직류양환단백（Trx）-2표체。실험수거비교채용단인소방차분석급LSD-t검험。결과4 h조위점막심층급점막하층충혈、수종、출혈，선체배렬문란，부분구역출혈、배사，미란심체점막기층；8、16 h조위점막손상감경，부점막천층화심층충혈、수종，Sham조표현위다수위점막상피결구완정。4 h조O2-화MDA분별위（185±26） U/mg화（0.4±0.1） nmol/mg，8 h조상응위（192±59）U/mg화（0.5±0.1）nmol/mg，명현고우Sham조적（102±34）U/mg화（0.2±0.1）nmol/mg（LSD-t=4.99，4.23화6.37，4.52；P<0.05）。4 h조GSH화GSH-PX분별위（17±6）mg/g화（781±174）U/mg，8 h조상응위（15±4）mg/g화（750±160）U/mg，명현저우Sham조적（30±6）mg/g화（1162±215）U/mg（LSD-t=-3.26，-4.01화-3.78，-4.36；P<0.05）。16 h조대서위조직O2-、MDA、GSH、GSH-PX분별위（169±27）U/mg、（0.3±0.1）nmol/mg、（25±8）mg/g、（1108±183）U/mg，여8 h조비교차이유통계학의의（LSD-t=-2.85，-3.46，2.66，3.69；P<0.05）。4、8 h조Trx-2단백상대표체량분별위52±10、43±8，교Sham조적125±16명현감소（LSD-t=-5.34，-6.23；P<0.05）。16 h조Trx-2단백표체량위160±18，교8 h조명현승고（LSD-t=4.75， P<0.05）。결론대서간이식결혈-재관주손상조기즉인기위양화손상，기발생가능여Trx-2표체하강、활성양증다화궤체항양화능력하강유관。
ObjectiveTo investigate the occurrence rule and mechanism of ischemia-reperfusion injury (IRI)-induced gastric oxidative damage during perioperative period of liver transplantation.Methods Twenty-eight SD rats were randomized into 4 groups according to the random number table. The modelof orthotopic autologous liver transplantation was established in 3 groups, which were 4 h, 8 h, 16 h group according to the reperfusion time of the liver grafts. The other group was set as Sham group. Gastric tissues were stained with HE to observe pathological changes of gastric mucosal injury. Superoxide anion (O2-), malondialdehyde (MDA), glutathione (GSH) and glutathione peroxidase (GSH-PX) in gastric tissues were detected. The expression of thioredoxin (Trx)-2 in gastric tissues was detected by Western blot. The comparison on experimental data was conducted using one-way analysis of variance and LSD-t test.Results In 4 h group, congestion, edema and hemorrhage were observed in deep stratum and submucous stratum of stomach, as well as disorganized glands, regional hemorrhage and necrosis, and erosion was observed deep to the muscularis mucosa. In 8 h group and 16 h group, gastric mucosal injury was alleviated, and only congestion and edema in superifcial and deep layer were observed. In Sham group, the epithelium structure of most gastric mucosa was intact. The O2- and MDA of 4 h group were respectively (185±26) U/mg and (0.4±0.1) nmol/mg and those of 8 h group were respectively (192±59) U/mg and (0.5±0.1) nmol/mg, which were signiifcantly higher than (102±34) U/mg and (0.2±0.1) nmol/mg of Sham group (LSD-t=4.99, 4.23 and 6.37, 4.52;P<0.05). GSH and GSH-PX of 4 h group were respectively (17±6) mg/g and (781±174) U/mg and those of 8 h group were respectively (15±4) mg/g and (750±160) U/mg, which were signiifcantly lower than (30±6) mg/g and (1 162±215) U/mg of Sham group (LSD-t=-3.26,-4.01 and-3.78,-4.36;P<0.05). The O2-, MDA, GSH and GSH-PX of 16 h group were respectively (169±27) U/mg, (0.3±0.1) nmol/mg, (25±8) mg/g and (1 108±183) U/mg, and signiifcant difference was observed comparing with 8 h group (LSD-t=-2.85,-3.46, 2.66, 3.69;P<0.05). The relative expression of Trx-2 in 4 h group and 8 h group were respectively 52±10 and 43±8, which were signiifcantly lower than 125±16 of Sham group (LSD-t=-5.34,-6.23;P<0.05). The expression of Trx-2 in 16 h group was 160±18, which was signiifcantly higher than that of 8 h group (LSD-t=4.75,P<0.05). ConclusionIRI causes gastric oxidative damage in the early phase after liver transplantation in rats, which may be associated with the decrease of Trx-2 expression, increase of active oxygen and decrease in organic antioxidative ability.
