CAJ | 학술논문

目的：探讨siRNA抑制人宫颈癌基因2（HCCR-2）表达对胆管癌细胞增殖能力的影响。方法分别采用Pcmv6-AC-GFP/HCCR-2-siRNA慢病毒表达载体及空载体Pcmv6-AC-GFP对照质粒感染人胆管癌RBE细胞，建立siRNA组和对照组。采用Western blot法检测HCCR-2蛋白表达。采用MTT法检测胆管癌细胞体外增殖能力，小鼠皮下移植瘤模型检测胆管癌细胞体内增殖能力。两组实验数据比较采用t检验。结果 siRNA组和对照组人胆管癌RBE细胞的HCCR-2蛋白平均相对表达量分别为0.21±0.03、0.70±0.02。与对照组相比，siRNA组的HCCR-2蛋白相对表达量明显减少（t=-8.06，P<0.05）。感染24 h和48 h后，siRNA组人胆管癌细胞测得的吸光度（A）值分别为0.05±0.01、0.16±0.01，明显小于对照组的0.11±0.02、0.39±0.06（t=-8.80，-11.31；P<0.05）。皮下成瘤模型生长至30 d时， siRNA组小鼠的肿瘤体积为（106±28）mm3，明显小于对照组的（516±24）mm3（t=-29.80，P<0.05）。结论 siRNA沉默HCCR-2基因表达可抑制胆管癌细胞的增殖能力。
목적：탐토siRNA억제인궁경암기인2（HCCR-2）표체대담관암세포증식능력적영향。방법분별채용Pcmv6-AC-GFP/HCCR-2-siRNA만병독표체재체급공재체Pcmv6-AC-GFP대조질립감염인담관암RBE세포，건립siRNA조화대조조。채용Western blot법검측HCCR-2단백표체。채용MTT법검측담관암세포체외증식능력，소서피하이식류모형검측담관암세포체내증식능력。량조실험수거비교채용t검험。결과 siRNA조화대조조인담관암RBE세포적HCCR-2단백평균상대표체량분별위0.21±0.03、0.70±0.02。여대조조상비，siRNA조적HCCR-2단백상대표체량명현감소（t=-8.06，P<0.05）。감염24 h화48 h후，siRNA조인담관암세포측득적흡광도（A）치분별위0.05±0.01、0.16±0.01，명현소우대조조적0.11±0.02、0.39±0.06（t=-8.80，-11.31；P<0.05）。피하성류모형생장지30 d시， siRNA조소서적종류체적위（106±28）mm3，명현소우대조조적（516±24）mm3（t=-29.80，P<0.05）。결론 siRNA침묵HCCR-2기인표체가억제담관암세포적증식능력。
ObjectiveTo investigate the influence of human cervical cancer gene 2 (HCCR-2) expression inhibited by siRNA on the proliferation of cholangiocarcinoma cells.MethodsPcmv6-AC-GFP/HCCR-2-siRNA lentiviral vector and Pcmv6-AC-GFP control vector were used to infect RBE human cholangiocarcinoma cells to establish the siRNA group and the control group. HCCR-2 protein expression was detected by Western blot.In vitro proliferation of cholangiocarcinoma cells was detected by MTT method andIn vivoproliferation of cholangiocarcinoma cells was detected by mice subcutaneous implanted tumor model. The experimental data of two groups were compared usingt test.ResultsThe average relative expression of HCCR-2 protein in RBE human cholangiocarcinoma cells of the siRNA group and the controlgroup was respectively 0.21±0.03 and 0.70±0.02. Compared with the control group, the relative expression of HCCR-2 protein of the siRNA group was signiifcantly reduced (t=-8.06,P<0.05). After 24 h and 48 h infection, the absorbance (A) measured in human cholangiocarcinoma cells of the siRNA group was respectively 0.05±0.01 and 0.16±0.01, which were signiifcantly lower than 0.11±0.02 and 0.39±0.06 of the control group (t=-8.80,-11.31;P<0.05). By the 30th day of subcutaneous implanted tumor grew, the tumor volume of the mice in the siRNA group was (106±28) mm3, which was signiifcantly smaller than (516±24) mm3 of the mice in the control group (t=-29.80,P<0.05).ConclusionExpression of HCCR-2 silenced by siRNA may inhibit the proliferation of cholangiocarcinoma cells.