风湿病与关节炎
風濕病與關節炎
풍습병여관절염
Rheumatism and Arthritis
2015年
8期
28-32
,共5页
盛录%赖宏强%王艺茹%李孝栋%苏友新
盛錄%賴宏彊%王藝茹%李孝棟%囌友新
성록%뢰굉강%왕예여%리효동%소우신
骨关节炎,膝%袋泡熏洗剂%提取工艺%正交优化
骨關節炎,膝%袋泡熏洗劑%提取工藝%正交優化
골관절염,슬%대포훈세제%제취공예%정교우화
osteoarthritis,knee%Bagged Xigubikang for Fumigating and Washing%extraction pro-cess%orthogonal optimization
目的：优选用于膝骨关节炎的膝骨痹康袋泡熏洗剂的提取工艺。方法：采用L9（34）的正交试验法，利用高效液相色谱法和紫外分光光度法分别测定柚皮苷和乌头碱的含量，并以干膏得率、柚皮苷含量和乌头碱含量的加权综合评分作为工艺筛选的评价指标，考察膝骨痹康袋泡熏洗剂制剂的水提取加水量、煎煮时间和提取次数对提取工艺的影响，确定膝骨痹康袋泡熏洗剂的最优提取工艺。结果：膝骨痹康袋泡熏洗剂最优提取工艺为15倍加水量，煎煮3次，每次1 h，验证3批所得样品平均的干膏得率、柚皮苷含量和乌头碱含量分别为20.73%、102.5 mg、22.4 mg，与正交试验结果相符，达到优化效果。结论：优选的提取工艺简便、稳定、重复性好，可用于膝骨痹康袋泡熏洗剂的工业化生产，为该制剂的进一步研究奠定良好的基础。
目的：優選用于膝骨關節炎的膝骨痺康袋泡熏洗劑的提取工藝。方法：採用L9（34）的正交試驗法，利用高效液相色譜法和紫外分光光度法分彆測定柚皮苷和烏頭堿的含量，併以榦膏得率、柚皮苷含量和烏頭堿含量的加權綜閤評分作為工藝篩選的評價指標，攷察膝骨痺康袋泡熏洗劑製劑的水提取加水量、煎煮時間和提取次數對提取工藝的影響，確定膝骨痺康袋泡熏洗劑的最優提取工藝。結果：膝骨痺康袋泡熏洗劑最優提取工藝為15倍加水量，煎煮3次，每次1 h，驗證3批所得樣品平均的榦膏得率、柚皮苷含量和烏頭堿含量分彆為20.73%、102.5 mg、22.4 mg，與正交試驗結果相符，達到優化效果。結論：優選的提取工藝簡便、穩定、重複性好，可用于膝骨痺康袋泡熏洗劑的工業化生產，為該製劑的進一步研究奠定良好的基礎。
목적：우선용우슬골관절염적슬골비강대포훈세제적제취공예。방법：채용L9（34）적정교시험법，이용고효액상색보법화자외분광광도법분별측정유피감화오두감적함량，병이간고득솔、유피감함량화오두감함량적가권종합평분작위공예사선적평개지표，고찰슬골비강대포훈세제제제적수제취가수량、전자시간화제취차수대제취공예적영향，학정슬골비강대포훈세제적최우제취공예。결과：슬골비강대포훈세제최우제취공예위15배가수량，전자3차，매차1 h，험증3비소득양품평균적간고득솔、유피감함량화오두감함량분별위20.73%、102.5 mg、22.4 mg，여정교시험결과상부，체도우화효과。결론：우선적제취공예간편、은정、중복성호，가용우슬골비강대포훈세제적공업화생산，위해제제적진일보연구전정량호적기출。
Objective:To optimize the extraction process of Bagged Xigubikang for Fumigating and Washing.Methods:L9(34) orthogonal test method,high performance liquid chromatography and ultraviolet spectrophotometry were used to determine the contents of naringin and aconitine,and the rate of dry extract and the weighted scores of the contents of naringin and aconitine were used as evaluation index for screening process in order to study the effect of amount of water,decoction time and times of extraction on extraction technology and to optimize the extraction process of Bagged Xigubikang for Fumigating and Washing.Results:The optimal extraction process of Bagged Xigubikang for Fumigating and Washing was to add 15 times of water and decoct for 3 times,1 h each time.It was veriifed that the average rate of dry extract and the contents of naringin and aconitine of the 3 batches of samples were respectively 20.73%,102.5 mg and 22.4 mg,which was consistent to the orthogo-nal test result.Conclusion:The optimized extraction process is simple,stable and reproducible and can be used for the industrial production.It is a good foundation for further research.
