CAJ | 학술논문

目的：观察复方中药脑尔康及其拆方组对阿尔茨海默病（AD）模型小鼠皮层、海马中突触素（SYN），高级糖基化终产物受体（RAGE）的影响。方法：随机将70只昆明小鼠分为7组，每组10只，采用 ip 氯化铝（AlCl3）建立 AD 模型小鼠，学习记忆成绩测试后，空白组和模型组每天给予生理盐水灌胃；全方组及各拆方组每天分别给予相应药物灌胃（14．3 mL ? d－1），连续30d 。测定各组小鼠皮层和海马 SYN 、RAGE 表达水平。结果：各用药组与模型组比较，全方组及拆方1号组小鼠脑内 SYN 表达明显增多（P ＜0．01），各拆方组与全方组比较，拆方1号组与全方组 SYN 表达无明显差异；各用药组 RAGE 表达有不同程度的下降（P ＜0．01或 P ＜0．05），以全方组及拆方1号组效果最为明显，且二者之间拮抗 RAGE表达的结果无明显差异，并优于其他用药组。结论：1拆方1号组及脑尔康全方组促进 AD模型小鼠脑内 SYN 表达的作用明显优于其他拆方组；拆方1号组及脑尔康全方组抑制 AD模型小鼠脑内 RAGE 表达的作用明显优于其他拆方组；拆方1号组与全方组治疗作用无明显差异，且药味精简。
목적：관찰복방중약뇌이강급기탁방조대아이자해묵병（AD）모형소서피층、해마중돌촉소（SYN），고급당기화종산물수체（RAGE）적영향。방법：수궤장70지곤명소서분위7조，매조10지，채용 ip 록화려（AlCl3）건립 AD 모형소서，학습기억성적측시후，공백조화모형조매천급여생리염수관위；전방조급각탁방조매천분별급여상응약물관위（14．3 mL ? d－1），련속30d 。측정각조소서피층화해마 SYN 、RAGE 표체수평。결과：각용약조여모형조비교，전방조급탁방1호조소서뇌내 SYN 표체명현증다（P ＜0．01），각탁방조여전방조비교，탁방1호조여전방조 SYN 표체무명현차이；각용약조 RAGE 표체유불동정도적하강（P ＜0．01혹 P ＜0．05），이전방조급탁방1호조효과최위명현，차이자지간길항 RAGE표체적결과무명현차이，병우우기타용약조。결론：1탁방1호조급뇌이강전방조촉진 AD모형소서뇌내 SYN 표체적작용명현우우기타탁방조；탁방1호조급뇌이강전방조억제 AD모형소서뇌내 RAGE 표체적작용명현우우기타탁방조；탁방1호조여전방조치료작용무명현차이，차약미정간。
Objective :To observe the amelioration of Naoerkang and its splited prescription on the expression of SYN and RAGE in cortex and hippocampus of senile dementia mice model .Method :Seventy male Kunming mice were randomly divided into 7 groups with 10 for each group ,then ip AlCl3 solution 100 mg ? kg - 1 ,ip ,q .o .d for 40 days to induce the model .After the examination of their ability in learning and memory ,each mouse of the control group and model group had been forced to drink saline solution (0 .5 mL ? d - 1 ) ,the other groups had been forced to drink the re‐sponse medicine (14 .3 g ? kg - 1 ? d - 1 ) every day for 30 days .The positive expression of SYN and RAGE in right cortex , hippocampus ,and the dentate gyrus of the mice in each group were examined .Result :Compared with the model group , the expression of SYN was increased evidently ( P < 0 .01)in the mice of Naoerkang group and splited prescription 1 group ,while the expression of different levels increased only in other groups (P< 0 .05) .The expression of RAGE was decreased evidently (P < 0 .01 or P < 0 .05) in the mice of Naoerkang group and all of its decomposed recipes .There were no significant differences existed in splited prescription 1 and Naoerkang group .Conclusion :Decomposed recipe 1 and Naoerkang groups have a better effect of promoting the expression of SYN in brains of AD mice model than any other splited prescription .Decomposed recipe 1 and Naoerkang groups have a better effect of inhibiting the expression of RAGE in brains of AD mice model than any other splited prescription .There were no significant differences between the thera‐peutic effect in splited prescription 1 and Naoerkang group .