风湿病与关节炎
風濕病與關節炎
풍습병여관절염
Rheumatism and Arthritis
2015年
8期
9-14
,共6页
刘正奇%钟琴%杨良山%曹跃朋%张雄峰%安阳
劉正奇%鐘琴%楊良山%曹躍朋%張雄峰%安暘
류정기%종금%양량산%조약붕%장웅봉%안양
痛风性关节炎%细胞因子%苗药痛风停%尿酸盐%实验研究
痛風性關節炎%細胞因子%苗藥痛風停%尿痠鹽%實驗研究
통풍성관절염%세포인자%묘약통풍정%뇨산염%실험연구
gout%cytokine%Miao medicine Tongfengting(苗药痛风停)%monosodium urate%experimental study
目的：观察苗药痛风停对尿酸盐结晶诱导的SD模型大鼠急性痛风性关节炎的关节肿胀度、步态分级、滑膜病理、白细胞介素-1β（IL-1β）、IL-6及肿瘤坏死因子-α（TNF-α）的影响，探讨苗药痛风停对急性痛风性关节炎的作用机制。方法：将60只SD大鼠随机分为空白对照组，苗药痛风停低、中、高剂量组，双氯芬酸钠组，模型对照组，每组10只。除空白对照组外，其余分别建立以尿酸盐结晶诱导的大鼠急性痛风性关节炎模型，分别观察和测量造模前及造模后6，12，24，48 h大鼠关节横径，并计算关节肿胀度；观察大鼠24，48 h步态分级；造模后48 h处死大鼠，用酶联免疫吸附法测定细胞因子IL-1β、IL-6和TNF-α的表达。结果：各治疗组对大鼠关节肿胀度、步态分级、滑膜病理改变和IL-1β、IL-6、TNF-α的表达有不同程度的改善，苗药痛风停中、高剂量组与双氯芬酸钠组比较，差异无统计学意义（P>0.05）。结论：苗药痛风停能够改善尿酸盐结晶诱导的SD模型大鼠急性痛风性关节炎关节肿胀度、步态分级及滑膜病理改变；苗药痛风停降低尿酸盐结晶诱导的SD模型大鼠细胞因子IL-1β、IL-6及TNF-α的表达，以中、高剂量作用明显，并呈剂量依赖性。
目的：觀察苗藥痛風停對尿痠鹽結晶誘導的SD模型大鼠急性痛風性關節炎的關節腫脹度、步態分級、滑膜病理、白細胞介素-1β（IL-1β）、IL-6及腫瘤壞死因子-α（TNF-α）的影響，探討苗藥痛風停對急性痛風性關節炎的作用機製。方法：將60隻SD大鼠隨機分為空白對照組，苗藥痛風停低、中、高劑量組，雙氯芬痠鈉組，模型對照組，每組10隻。除空白對照組外，其餘分彆建立以尿痠鹽結晶誘導的大鼠急性痛風性關節炎模型，分彆觀察和測量造模前及造模後6，12，24，48 h大鼠關節橫徑，併計算關節腫脹度；觀察大鼠24，48 h步態分級；造模後48 h處死大鼠，用酶聯免疫吸附法測定細胞因子IL-1β、IL-6和TNF-α的錶達。結果：各治療組對大鼠關節腫脹度、步態分級、滑膜病理改變和IL-1β、IL-6、TNF-α的錶達有不同程度的改善，苗藥痛風停中、高劑量組與雙氯芬痠鈉組比較，差異無統計學意義（P>0.05）。結論：苗藥痛風停能夠改善尿痠鹽結晶誘導的SD模型大鼠急性痛風性關節炎關節腫脹度、步態分級及滑膜病理改變；苗藥痛風停降低尿痠鹽結晶誘導的SD模型大鼠細胞因子IL-1β、IL-6及TNF-α的錶達，以中、高劑量作用明顯，併呈劑量依賴性。
목적：관찰묘약통풍정대뇨산염결정유도적SD모형대서급성통풍성관절염적관절종창도、보태분급、활막병리、백세포개소-1β（IL-1β）、IL-6급종류배사인자-α（TNF-α）적영향，탐토묘약통풍정대급성통풍성관절염적작용궤제。방법：장60지SD대서수궤분위공백대조조，묘약통풍정저、중、고제량조，쌍록분산납조，모형대조조，매조10지。제공백대조조외，기여분별건립이뇨산염결정유도적대서급성통풍성관절염모형，분별관찰화측량조모전급조모후6，12，24，48 h대서관절횡경，병계산관절종창도；관찰대서24，48 h보태분급；조모후48 h처사대서，용매련면역흡부법측정세포인자IL-1β、IL-6화TNF-α적표체。결과：각치료조대대서관절종창도、보태분급、활막병리개변화IL-1β、IL-6、TNF-α적표체유불동정도적개선，묘약통풍정중、고제량조여쌍록분산납조비교，차이무통계학의의（P>0.05）。결론：묘약통풍정능구개선뇨산염결정유도적SD모형대서급성통풍성관절염관절종창도、보태분급급활막병리개변；묘약통풍정강저뇨산염결정유도적SD모형대서세포인자IL-1β、IL-6급TNF-α적표체，이중、고제량작용명현，병정제량의뢰성。
Objective:To observe the effect of Miao medicine Tongfengting (苗药痛风停) on joint swelling,gait grading,pathological change of synovium,and the expression of IL-1β,IL-6 and TNF-α of SD rats with acute gout arthritis induced by monosodium urate crystal in order to study the mechanism of action of the medicine on acute gout arthritis.Methods:60 SD rats were randomly divided into a blank control group,low,medium and high dose groups of Miao medicine Tongfengting,a diclofenac sodium group and a model control group,10 rats in each group.Apart from the control group,models of acute gout arthritis induced bymonosodium urate crystal were established. The joint transverse diameter of model rats was observed and the joint swelling was measured respectively after 6,12,24 and 48 hours.The model rats were killed after 48 hours to measure the expression of IL-1β,IL-6 and TNF-α. Results:The joint swelling,gait grading,pathological change of synovium,IL-1β,IL-6 and TNF-α of SD rats all had different degrees of improvement in each treatment group.The difference between the medium and high dose groups and the diclofenac sodium group was not statistically signiifcant (P>0.05).Conclusion:Miao medicine Tongfengting can improve the joint swelling,gait grading and pathological change of synovium and lower the expression of IL-1β,IL-6 and TNF-α which was obvious and dose dependent in the medium and high dose groups.
