风湿病与关节炎
風濕病與關節炎
풍습병여관절염
Rheumatism and Arthritis
2015年
8期
5-8,14
,共5页
卞华%徐艳琴%韩立%叶松山%陈丽平%张培华
卞華%徐豔琴%韓立%葉鬆山%陳麗平%張培華
변화%서염금%한립%협송산%진려평%장배화
系统性硬化病%温阳化浊通络方%皮肤成纤维细胞%表型转化%迁移%Wnt-1
繫統性硬化病%溫暘化濁通絡方%皮膚成纖維細胞%錶型轉化%遷移%Wnt-1
계통성경화병%온양화탁통락방%피부성섬유세포%표형전화%천이%Wnt-1
systemic sclerosis%Wenyang Huazhuo Tongluo Prescription(温阳化浊通络方)%skin if-broblasts%phenotype transformation%migra-tion%Wnt-1
目的：探讨温阳化浊通络方含药血清对Wnt-1诱导的人皮肤成纤维细胞表型转化及迁移的影响。方法：通过对Wistar大鼠灌胃制备温阳化浊通络方含药血清，体外培养正常人皮肤成纤维细胞，分为空白对照组、Wnt组和温阳化浊通络方组，作用24，48，72 h后，分别用RT-PCR和Western blot法检测α-SMA mRNA及蛋白的表达，Transwell试验检测成纤维细胞迁移情况。结果：与空白对照组比较， Wnt组在不同作用时间下能诱导人皮肤成纤维细胞α-SMA mRNA及蛋白表达（P<0.01），增加其迁移数量（P<0.01）；与Wnt组比较，温阳化浊通络方组在不同作用时间下能够显著抑制Wnt-1诱导的人皮肤成纤维细胞α-SMA mRNA及蛋白表达（P<0.05，P<0.01），同时阻止其迁移（P<0.01）。结论：温阳化浊通络方含药血清能抑制Wnt-1诱导的人皮肤成纤维细胞表型转化及迁移，具有阻止或延缓系统性硬化病纤维化的潜能。
目的：探討溫暘化濁通絡方含藥血清對Wnt-1誘導的人皮膚成纖維細胞錶型轉化及遷移的影響。方法：通過對Wistar大鼠灌胃製備溫暘化濁通絡方含藥血清，體外培養正常人皮膚成纖維細胞，分為空白對照組、Wnt組和溫暘化濁通絡方組，作用24，48，72 h後，分彆用RT-PCR和Western blot法檢測α-SMA mRNA及蛋白的錶達，Transwell試驗檢測成纖維細胞遷移情況。結果：與空白對照組比較， Wnt組在不同作用時間下能誘導人皮膚成纖維細胞α-SMA mRNA及蛋白錶達（P<0.01），增加其遷移數量（P<0.01）；與Wnt組比較，溫暘化濁通絡方組在不同作用時間下能夠顯著抑製Wnt-1誘導的人皮膚成纖維細胞α-SMA mRNA及蛋白錶達（P<0.05，P<0.01），同時阻止其遷移（P<0.01）。結論：溫暘化濁通絡方含藥血清能抑製Wnt-1誘導的人皮膚成纖維細胞錶型轉化及遷移，具有阻止或延緩繫統性硬化病纖維化的潛能。
목적：탐토온양화탁통락방함약혈청대Wnt-1유도적인피부성섬유세포표형전화급천이적영향。방법：통과대Wistar대서관위제비온양화탁통락방함약혈청，체외배양정상인피부성섬유세포，분위공백대조조、Wnt조화온양화탁통락방조，작용24，48，72 h후，분별용RT-PCR화Western blot법검측α-SMA mRNA급단백적표체，Transwell시험검측성섬유세포천이정황。결과：여공백대조조비교， Wnt조재불동작용시간하능유도인피부성섬유세포α-SMA mRNA급단백표체（P<0.01），증가기천이수량（P<0.01）；여Wnt조비교，온양화탁통락방조재불동작용시간하능구현저억제Wnt-1유도적인피부성섬유세포α-SMA mRNA급단백표체（P<0.05，P<0.01），동시조지기천이（P<0.01）。결론：온양화탁통락방함약혈청능억제Wnt-1유도적인피부성섬유세포표형전화급천이，구유조지혹연완계통성경화병섬유화적잠능。
Objective:To investigate the effects of Wenyang Huazhuo Tongluo Prescription(温阳化浊通络方)on the phenotype transformation and migration of human skin ifbroblast induced by Wnt-1.Methods:Medicated serum of Wistar rats was got by intragastric administration of Wenyang Huazhuo Tongluo Prescription and normal human skin fibroblasts were cultured in vitro.The rats were divided into a blank control group,a Wnt group and a Wenyang Huazhuo Tongluo Prescription group.Respectively after 24,48,72 hours,RT-PCR and Western blot were used to detect the expressions of α-SMA mRNA and protein,and Transwell was used to detect the migration of fibroblasts.Results:Compared with the blank control group,human skin fibroblasts a-SMA mRNA and protein expression in the Wnt group could be induced in different time(P<0.01),and the number of migration increased(P<0.01).Compared with the Wnt group,human skin ifbroblasts a-SMA mRNA and protein expression in the Wenyang Huazhuo Tongluo Prescription group were obviously inhibited(P < 0.05, P<0.01) and their migration were prevented(P<0.01).Conclusion:Medicated serum of Wenyang Huazhuo Tongluo Prescription can inhibit the phenotype transformation and migration of human skin ifbroblast induced by Wnt-1,having the potential to prevent or delay systemic sclerosis ifbrosis.
