CAJ | 학술논문

目的：探讨非转移乳腺癌患者外周血中游离无细胞的DNA( cfDNA)中ErbB-2基因启动子区5’CpG甲基化状态及其与肿瘤组织中Her-2表达的关系。方法分离非转移乳腺癌患者外周血中的cfDNA,利用重亚硫酸盐测序法( BSP)对分离出的cfDNA及外周血细胞DNA的ErbB-2基因启动子区进行5’CpG甲基化分析,免疫组化及FISH方法检测肿瘤组织中Her-2的表达情况。结果 ErbB-2基因启动子区5’CpG的甲基化只在乳腺癌患者的血浆cfDNA中检测到(19/40),在其对应的外周血血细胞中未发现；乳腺癌患者外周血cfDNA的ErbB-2基因启动子区5’CpG甲基化和其肿瘤组织Her-2的表达成负相关。结论乳腺癌患者外周血cfDNA ErbB-2启动子区5’CpG的甲基化状态与肿瘤组织Her-2表达呈负相关。提示外周血cfDNA ErbB-2启动子区5’ CpG甲基化状态能从某种程度上反映乳腺癌患者肿瘤组织Her-2表达情况,有可能作为乳腺癌Her-2状态观察的新窗口,可用于帮助乳腺癌分子分型判断,是否能作为乳腺癌治疗的新靶点,并作为Her-2阳性患者早期诊断的指标,尚有待于进一步研究。
목적：탐토비전이유선암환자외주혈중유리무세포적DNA( cfDNA)중ErbB-2기인계동자구5’CpG갑기화상태급기여종류조직중Her-2표체적관계。방법분리비전이유선암환자외주혈중적cfDNA,이용중아류산염측서법( BSP)대분리출적cfDNA급외주혈세포DNA적ErbB-2기인계동자구진행5’CpG갑기화분석,면역조화급FISH방법검측종류조직중Her-2적표체정황。결과 ErbB-2기인계동자구5’CpG적갑기화지재유선암환자적혈장cfDNA중검측도(19/40),재기대응적외주혈혈세포중미발현；유선암환자외주혈cfDNA적ErbB-2기인계동자구5’CpG갑기화화기종류조직Her-2적표체성부상관。결론유선암환자외주혈cfDNA ErbB-2계동자구5’CpG적갑기화상태여종류조직Her-2표체정부상관。제시외주혈cfDNA ErbB-2계동자구5’ CpG갑기화상태능종모충정도상반영유선암환자종류조직Her-2표체정황,유가능작위유선암Her-2상태관찰적신창구,가용우방조유선암분자분형판단,시부능작위유선암치료적신파점,병작위Her-2양성환자조기진단적지표,상유대우진일보연구。
Objective To investigate the relationship of methylation of ErbB-2 gene 5 ’ CpG of cfDNA in peripheral blood of non-metastatic breast cancer patients with expression of Her-2 in tumor tissue. Methods I-solate cell-free DNA in peripheral blood of non-metastatic breast cancer patients, analysis methylation of 5’CpG in ErbB-2 gene promoter using bisulfite-sequencing PCR ( BSP) , detect the expression of Her-2 using immuno-histochemistry and FISH. Results Methylation of ErbB-2 gene promoter was only detected in plasma cfDNA of breast cancer patients (19/40), not found in the corresponding peripheral blood cells; Methylation of ErbB-2 gene 5’CpG of cfDNA in peripheral blood of breast cancer patients was negatively correlated with the expression of Her-2 in tumor tissue. Conclusions Methylation of ErbB-2 gene 5’CpG in peripheral blood of breast cancer patients was negatively correlated with the expression of Her-2 in tumor tissue. We can speculate that the methy-lation of ErbB-2 gene 5 ’ CpG in peripheral blood of breast cancer patients can reflect the expression of Her-2 breast cancer tissues to some extent, which may be used as a new method to observe Her-2 status of breast canc-er, and which also can be used to help determine the molecular typing of breast cancer, and used as a new tar-get for breast cancer treatment, and as an early diagnosis indicator of Her-2 positive patients still needs further study.