CAJ | 학술논문

目的：观察小鼠慢性酒精性肝病引起的肝细胞聚核糖性死亡的细胞学变化特点。方法应用免疫印迹法、免疫组织化学染色方法和光学、电子显微镜技术对小鼠慢性酒精性肝病的肝脏进行了观察。结果实验性酒精损伤的动物发生了肝功能受损，肝脏出现了大小不等坏死灶。肝细胞呈现出坏死表现。细胞肿胀，细胞内大量空泡形成，核萎缩变形。免疫组化染色和免疫印迹的结果说明在肝细胞损伤过程中多聚二磷酸腺苷核糖聚合酶－1（PARP－1）过度激活。结论小鼠慢性酒精性肝病可引起肝细胞坏死。肝细胞聚核糖性死亡（PARP－1介导的细胞死亡）参与了这种肝细胞损伤。
목적：관찰소서만성주정성간병인기적간세포취핵당성사망적세포학변화특점。방법응용면역인적법、면역조직화학염색방법화광학、전자현미경기술대소서만성주정성간병적간장진행료관찰。결과실험성주정손상적동물발생료간공능수손，간장출현료대소불등배사조。간세포정현출배사표현。세포종창，세포내대량공포형성，핵위축변형。면역조화염색화면역인적적결과설명재간세포손상과정중다취이린산선감핵당취합매－1（PARP－1）과도격활。결론소서만성주정성간병가인기간세포배사。간세포취핵당성사망（PARP－1개도적세포사망）삼여료저충간세포손상。
Objective To observe the parthanatos of hepatocyte in mice with alcoholic liver disease.Methods The liver sam-ples of the mice with chronic alcoholic liver disease was observed by Western blot, immunohistochemical staining and optical and elec-tron microscopy.Results Liver damage and necrosis lesions was predominately found in mice with alcoholic liver injury.Necrosis of mice's hepatocytes happened.The cells were found swelling and a large number of vacuoles were formed in the cell; the nucleus was a-trophic and deformed.Results of immunohistochemical staining and Western blot showed that the expression of PARP-1 in the process of liver cell injury was over-activated.Conclusion Parthanatos of hepatocyte is involved in hepatic damages induced by alcohol which may cause necrosisof hepatocytes.