CAJ | 학술논문

本试验旨在研究京红蛋鸡对饲粮硫酸钠的耐受剂量。采用单因子随机区组设计,选用健康、体重均匀的21周龄京红蛋鸡360只,随机分为5个组(每个组6个重复,每个重复12只鸡)。分别饲喂添加0、0.6%、1.2%、1.8%和3.0%硫酸钠的饲粮,预试期1周,正试期8周。结果显示：试验后期(第5~8周),1.8%和3.0%组产蛋率降低,与0.6%组差异显著(P<0.05)。第4周,硫酸钠对蛋壳强度、蛋白高度、哈氏单位均有显著影响( P<0.05)；0.6%组蛋壳强度最佳,极显著高于其他组( P<0.01)。第8周,1.8%和3.0%蛋壳强度极显著低于对照组( P<0.01)。饲粮中添加硫酸钠显著降低血液钾离子含量(P<0.05),并有提高氯离子含量的趋势(P<0.10)。3.0%组红细胞数量极显著高于对照组( P<0.01)。生化指标中,3.0%组的血液总蛋白、白蛋白、葡萄糖含量均显著低于其他各组( P<0.05)。器官切片观察可见,1.8%和3.0%组肾小球水肿、肾小囊萎缩、肾间质发炎,肠道炎症。综上,饲粮中添加硫酸钠≥1.8%,会损害产蛋鸡的健康生长及生产,因此京红蛋鸡对饲粮硫酸钠的耐受剂量为1.2%。
본시험지재연구경홍단계대사량류산납적내수제량。채용단인자수궤구조설계,선용건강、체중균균적21주령경홍단계360지,수궤분위5개조(매개조6개중복,매개중복12지계)。분별사위첨가0、0.6%、1.2%、1.8%화3.0%류산납적사량,예시기1주,정시기8주。결과현시：시험후기(제5~8주),1.8%화3.0%조산단솔강저,여0.6%조차이현저(P<0.05)。제4주,류산납대단각강도、단백고도、합씨단위균유현저영향( P<0.05)；0.6%조단각강도최가,겁현저고우기타조( P<0.01)。제8주,1.8%화3.0%단각강도겁현저저우대조조( P<0.01)。사량중첨가류산납현저강저혈액갑리자함량(P<0.05),병유제고록리자함량적추세(P<0.10)。3.0%조홍세포수량겁현저고우대조조( P<0.01)。생화지표중,3.0%조적혈액총단백、백단백、포도당함량균현저저우기타각조( P<0.05)。기관절편관찰가견,1.8%화3.0%조신소구수종、신소낭위축、신간질발염,장도염증。종상,사량중첨가류산납≥1.8%,회손해산단계적건강생장급생산,인차경홍단계대사량류산납적내수제량위1.2%。
The current study was conducted with the aim to explore the tolerant dosage of dietary Na2SO4 in laying hens. Three hundred and sixty 21-week-age Jinghong laying hens were randomly assigned to 5 groups and were fed based diet with 0 (control), 0.6%, 1.2%, 1.8%, 3.0% Na2SO4 for 8 weeks, respectively. Each group consisted of 6 replicates per group and 12 hens per replicate. The pre-test lasted for 1 week, and the test lasted for 8 weeks. The results showed as follows: in the later stage ( from 5 to 8 week) , layers fed 1.8% or 3. 0% Na2SO4 diet had significantly increased ratio of feed to egg compared with those fed 0. 6%Na2SO4(P<0. 05). On week 4 of experiment, dietary 0. 6% Na2SO4 significantly increased the eggshell strength, albumen height and Haugh unit than other groups (P<0.01). On week 8 of experiment, layers in 1.8%, 3.0% Na2SO4 group had significantly decreased eggshell strength (P<0.01). Dietary Na2SO4 markedly reduced the content of K+ in blood ( P<0.05) , and partially increased the content of Cl- in blood ( P<0.10) on week 8 of experiment. Compare with the control group, 3.0% Na2SO4 group significantly increased the count of red blood cell. The concentration of blood total protein, albumin, glucose were significantly decreased by the supplementation of 3.0% Na2SO4(P<0.05). The morphology assay of different organs showed that lay-ers fed 1.8% or 3.0% Na2SO4 diet had glomerular atrophy, inflammatory cells immersed in villous stroma of kidney and in intestine. In conclusion, it is damaged to health, growth and production of hens when diet adding with 1.8% Na2SO4, so the tolerant dose of the Na2SO4 to Jinghong laying hens can be recommended as 1.2%in diet.