CAJ | 학술논문

本试验旨在研究饲粮添加不同水平α-半乳糖苷酶对1~21日龄爱拔益加( AA)肉鸡生长性能、肠道黏膜形态结构和血清生化、激素指标的影响。试验选择1日龄体重相近的健康AA肉鸡360羽，随机分成6个处理，每处理5个重复，每重复12羽，处理A饲喂玉米-豆粕型基础饲粮作为正对照，处理B饲喂降低基础饲粮代谢能0.21 MJ/kg的饲粮作为负对照，处理C、D、E、F分别饲喂在负对照处理饲粮中添加75、150、300、600 g/t的α-半乳糖苷酶制剂(酶活为400 U/g)的饲粮。试验期为21 d。结果表明：1)饲粮中添加不同水平α-半乳糖苷酶对1~21日龄各处理各阶段AA肉鸡平均日增重( ADG)、平均日采食量( ADFI)、料重比( F/G)以及1~21日龄试鸡存活率均无显著影响( P>0.05)，但饲粮添加75 g/t的α-半乳糖苷酶(处理C)有改善1~21日龄AA肉鸡F/G的趋势(0.10>P>0.05)；2)酶制剂的添加不同程度地促进了1~21日龄AA肉鸡十二指肠、空肠和回肠黏膜形态结构的发育；3)饲粮中添加不同水平α-半乳糖苷酶较正、负对照处理对21日龄AA肉鸡血清总蛋白、球蛋白含量和白蛋白/球蛋白影响显著( P<0.05)，同时处理C、E、F较负对照处理对21日龄AA肉鸡血清胰岛素含量有极显著提高( P<0.01)，处理C和E试鸡21日龄血清胰高血糖素含量较正对照处理亦有极显著提高( P<0.01)。综合分析生长性能、肠道黏膜形态结构和血清生化、激素指标结果，在本试验条件下，在降低基础饲粮代谢能0.21 MJ/kg的基础上，添加75 g/t的α-半乳糖苷酶制剂即可使1~21日龄AA肉鸡生长性能达到与饲喂基础饲粮相当的水平。
본시험지재연구사량첨가불동수평α-반유당감매대1~21일령애발익가( AA)육계생장성능、장도점막형태결구화혈청생화、격소지표적영향。시험선택1일령체중상근적건강AA육계360우，수궤분성6개처리，매처리5개중복，매중복12우，처리A사위옥미-두박형기출사량작위정대조，처리B사위강저기출사량대사능0.21 MJ/kg적사량작위부대조，처리C、D、E、F분별사위재부대조처리사량중첨가75、150、300、600 g/t적α-반유당감매제제(매활위400 U/g)적사량。시험기위21 d。결과표명：1)사량중첨가불동수평α-반유당감매대1~21일령각처리각계단AA육계평균일증중( ADG)、평균일채식량( ADFI)、료중비( F/G)이급1~21일령시계존활솔균무현저영향( P>0.05)，단사량첨가75 g/t적α-반유당감매(처리C)유개선1~21일령AA육계F/G적추세(0.10>P>0.05)；2)매제제적첨가불동정도지촉진료1~21일령AA육계십이지장、공장화회장점막형태결구적발육；3)사량중첨가불동수평α-반유당감매교정、부대조처리대21일령AA육계혈청총단백、구단백함량화백단백/구단백영향현저( P<0.05)，동시처리C、E、F교부대조처리대21일령AA육계혈청이도소함량유겁현저제고( P<0.01)，처리C화E시계21일령혈청이고혈당소함량교정대조처리역유겁현저제고( P<0.01)。종합분석생장성능、장도점막형태결구화혈청생화、격소지표결과，재본시험조건하，재강저기출사량대사능0.21 MJ/kg적기출상，첨가75 g/t적α-반유당감매제제즉가사1~21일령AA육계생장성능체도여사위기출사량상당적수평。
This study was conducted to evaluate the effects of different levels ofα-galactosidase on growth per-formance, intestinal mucosa structure, and serum biochemical and hormonal indices of Arbor Acre ( AA ) broilers aged from 1 to 21 days. A total of 360 healthy 1-day-old AA broilers with the nearly same weight were randomly selected and allocated to 6 treatments, 5 replicates per treatment and 12 broilers per replication. The positive control treatment was provided with the corn-soybean basal diet, the negative control treatment was fed the basal diet which was reduced 0.21 MJ/kg ME, and the test treatments C, D, E and F were fed the nega-tive control diet which were added 75, 150, 300, 600 g/tα-galactosidase ( enzyme activities was 400 U/g) , respectively. The experiment period was 21 d. The results showed as follows: 1 ) there was no significant difference on average daily gain ( ADG) , average daily feed intake ( ADFI) , the ratio of feed to gain ( F/G) and survival rate during the whole experiment period (P>0.05), but there was a trend that the addition of 75 g/t α-galactosidase (treatment C) decreased F/G of AA broilers aged from 1 to 21 days (0.10>P>0.05). 2) The addition of α-galactosidase could improve the mucosa structure development of duodenum, jejunum and ileum in some extent. 3) Compared with treatments A and B, Added different levels of α-galactosidase in diet significantly affected the contents of serum total protein ( TP) , globulin ( GLO) and the ratio of albumin to globulin ( ALB/GLO) on 21-day-old broilers ( P<0.05) . And at the same time, compared with treatments B, treatments C, E and F significantly improved the content of serum insulin of 21-day-old broilers ( P<0.01) , and compared with treatment A, treatments C and E also significantly improved the content of glucagon (GLU) of 21-day-old broilers (P<0.01). In general, added 75 g/t α-galactosidase had a good effect when AA broilers aged from 1 to 21 d fed the diet which was reduced 0.21 MJ/kg ME.