CAJ | 학술논문

目的：探讨微电场（electric field，EF）刺激血管内皮细胞培养上清对缺氧培养滋养细胞迁移／侵袭能力的影响。方法200 mV／mm 微电场刺激血管内皮细胞（HUVEC）5 h，收集培养上清。 Transwell 体外侵袭实验和划痕实验观察 EF 刺激内皮细胞条件培养基对3％ O2物理缺氧培养滋养细胞迁移／侵袭功能的影响。采用 Western blot 和荧光定量 RT－PCR 检测 EF刺激的血管内皮细胞条件培养基与滋养细胞共培养24 h 后对缺氧培养滋养细胞 MMP－2（Matrix metalloproteinase 2，MMP－2）及MMP－9（Matrix metalloproteinase 9，MMP－9）分子表达的影响。结果Transwell 小室体外侵袭实验和划痕实验结果显示，EF 200 mV／mm 刺激血管内皮细胞5 h 条件培养基（conditioned medium，CM）与滋养细胞共培养，可促进缺氧培养滋养细胞迁移／侵袭功能，其穿膜细胞数目是单纯缺氧的3．62倍（P ＜0．05），迁移速度是单纯缺氧的1．56倍（P ＜0．05）。 EF 刺激的血管内皮细胞条件培养基与滋养细胞共培养24 h，可以促进缺氧培养滋养细胞的 MMP－2表达；对 MMP－9分子表达的影响不明显。结论EF 刺激的血管内皮细胞培养上清能促进缺氧培养滋养细胞的迁移／侵袭能力，可能与内皮细胞作用于滋养细胞、促进 MMP－2分子表达增加有关。
목적：탐토미전장（electric field，EF）자격혈관내피세포배양상청대결양배양자양세포천이／침습능력적영향。방법200 mV／mm 미전장자격혈관내피세포（HUVEC）5 h，수집배양상청。 Transwell 체외침습실험화화흔실험관찰 EF 자격내피세포조건배양기대3％ O2물리결양배양자양세포천이／침습공능적영향。채용 Western blot 화형광정량 RT－PCR 검측 EF자격적혈관내피세포조건배양기여자양세포공배양24 h 후대결양배양자양세포 MMP－2（Matrix metalloproteinase 2，MMP－2）급MMP－9（Matrix metalloproteinase 9，MMP－9）분자표체적영향。결과Transwell 소실체외침습실험화화흔실험결과현시，EF 200 mV／mm 자격혈관내피세포5 h 조건배양기（conditioned medium，CM）여자양세포공배양，가촉진결양배양자양세포천이／침습공능，기천막세포수목시단순결양적3．62배（P ＜0．05），천이속도시단순결양적1．56배（P ＜0．05）。 EF 자격적혈관내피세포조건배양기여자양세포공배양24 h，가이촉진결양배양자양세포적 MMP－2표체；대 MMP－9분자표체적영향불명현。결론EF 자격적혈관내피세포배양상청능촉진결양배양자양세포적천이／침습능력，가능여내피세포작용우자양세포、촉진 MMP－2분자표체증가유관。
Objective To investigate the effect of conditioned medium (CM)obtained from micro direct-current(DC)electrical field(EF) stimulated vascular endothelial cell on migration /invasion of trophoblast cells under hypoxia condition .Methods Human vascular endothelial cells(HUVECs)were exposed to the DC-EF at 200 mV/mm for 4 ~24 hours,and the conditioned medium (CM) were collected.The transwell assays were used for measuring the effect of EF stimulated HUVECs CM on trophoblasts invasion under the conditions of normal oxygen or hypoxia .Results The CM from EF stimulation for 5 hr could enhance trophoblast invasion .There were 3.62-fold increases in invasion cell numbers (P <0.05)and 1.56-fold in velocity(P <0.05)when compared with plain medium under hypoxia condition by transwell assay and wound healing assay .Western blot and quantitative RT-PCR assays showed that the CM could enhance the expression of MMP-2 but not MMP-9 of trophoblast cells at both transcription and translation levels .Conclusion The CM from EF stimulated vascular endothelial cells could enhance migration /invasion of trophoblast cells under hypoxia condition ,and this effect might be in part related to the enhanced expression of MMP -2.