CAJ | 학술논문

目的：探讨布洛芬对兔骨关节炎（osteoarthritis，OA）模型膝关节软骨细胞基质代谢及 Wnt ／β－catenin 信号通路的影响。方法采用改良伸直位固定法制作兔 OA 模型，分离提取兔 OA 模型膝关节软骨细胞进行培养。采用四唑盐比色法选择布洛芬和二甲基亚砜（DMSO）实验浓度。确定实验浓度后将软骨细胞分为 DMSO 组和布洛芬组，分别给予1％DMSO 和250μM 布洛芬干预48 h 后，采用 real－time PCR 法和 ELISA 方法检测软骨细胞 Wnt ／β－catenin 相关因子（β－catenin、MMP－13、ADAMTS－4、II 型胶原和蛋白聚糖）mRNA 及蛋白的水平。结果DMSO 对照组和布洛芬组β－catenin、MMP－13、ADAMTS－4、II型胶原和蛋白聚糖的 mRNA 及蛋白水平比较，差异均无统计学意义（P ＞0．05）。结论兔 OA 模型膝关节软骨细胞中，布洛芬对 Wnt ／β－catenin 信号通路和软骨细胞基质代谢无明显影响。
목적：탐토포락분대토골관절염（osteoarthritis，OA）모형슬관절연골세포기질대사급 Wnt ／β－catenin 신호통로적영향。방법채용개량신직위고정법제작토 OA 모형，분리제취토 OA 모형슬관절연골세포진행배양。채용사서염비색법선택포락분화이갑기아풍（DMSO）실험농도。학정실험농도후장연골세포분위 DMSO 조화포락분조，분별급여1％DMSO 화250μM 포락분간예48 h 후，채용 real－time PCR 법화 ELISA 방법검측연골세포 Wnt ／β－catenin 상관인자（β－catenin、MMP－13、ADAMTS－4、II 형효원화단백취당）mRNA 급단백적수평。결과DMSO 대조조화포락분조β－catenin、MMP－13、ADAMTS－4、II형효원화단백취당적 mRNA 급단백수평비교，차이균무통계학의의（P ＞0．05）。결론토 OA 모형슬관절연골세포중，포락분대 Wnt ／β－catenin 신호통로화연골세포기질대사무명현영향。
Objective To study the metabolism how ibuprofen influence matrix metabolism of rabbit OA chondrocytes and the role of Wnt /β-catenin signaling pathway in this process .Methods Use a modified way of mechanical fixation to make rabbit OA mod -el,separation and extraction of rabbit knee OA chondrocytes and then culture in medium .MTT was used to select the dose of DMSO and ibuprofen.After that,the cultured chondroctyes were divided into nomral control group and ibuprofen group ,use ELISA and real-time PCR to detect the protein and gene expression of Wnt /β-catenin signaling pathway related factors including β-catenin,MMP-3,MMP-13,ADAMTS-4,collagen II,proteoglycan.Results There were no differences between normal group and ibuprofen group of Wnt /β-catenin signaling pathway related factors including β-catenin,MMP-3,MMP-13,ADAMTS-4,collagen II and proteoglycan .Conclusion In rabbit OA knee chondrocytes ,ibuprofen has no effect on matrix metabolism and Wnt /β-catenin signaling pathway.