CAJ | 학술논문

采用SSR和SRAP两种标记分析了来自赤峰地区25份和国家种质资源库10份皮燕麦材料的遗传多样性.19对SSR引物和25对SRAP引物分别扩增出32个和259个位点,其中多态性位点分别为30个和199个,多态性百分率分别为93.8%和76.8%. SSR标记位点多态性信息含量(PIC)变幅为0.665 4~0.798 3,平均为0.755 6;有效等位基因数(Ne)变幅为1.159 2~1.971 1,平均为1.751 4;香农指数(H′)变幅为0.264 3~0.836 2,平均为0.596 9.SRAP标记位点多态性信息含量(PIC)变幅为0.354 6~0.932 1,平均为0.743 0;有效等位基因数(Ne)变幅为1.102 4~1.992 8,平均为1.672 4;香农指数(H′)变幅为0.168 2~0.691 4,平均为0.555 6. 35份皮燕麦材料之间的遗传相似系数变化幅度为0.46~0.95,在遗传相似系数0.70处,可分成4组,同一来源的材料都分布在多个组中,赤峰地区皮燕麦资源存在丰富的遗传多样性,今后应加强皮燕麦种质资源收集、保存、鉴定、利用等工作.
채용SSR화SRAP량충표기분석료래자적봉지구25빈화국가충질자원고10빈피연맥재료적유전다양성.19대SSR인물화25대SRAP인물분별확증출32개화259개위점,기중다태성위점분별위30개화199개,다태성백분솔분별위93.8%화76.8%. SSR표기위점다태성신식함량(PIC)변폭위0.665 4~0.798 3,평균위0.755 6;유효등위기인수(Ne)변폭위1.159 2~1.971 1,평균위1.751 4;향농지수(H′)변폭위0.264 3~0.836 2,평균위0.596 9.SRAP표기위점다태성신식함량(PIC)변폭위0.354 6~0.932 1,평균위0.743 0;유효등위기인수(Ne)변폭위1.102 4~1.992 8,평균위1.672 4;향농지수(H′)변폭위0.168 2~0.691 4,평균위0.555 6. 35빈피연맥재료지간적유전상사계수변화폭도위0.46~0.95,재유전상사계수0.70처,가분성4조,동일래원적재료도분포재다개조중,적봉지구피연맥자원존재봉부적유전다양성,금후응가강피연맥충질자원수집、보존、감정、이용등공작.
We studied the genetic diversity of 35 hulled oat accessions using SSR and SRAP markers in this paper. Nineteen SSR primer pairs and 26 SRAP primer combinations were used, and 32 and 259 loci were amplified, of which 30 (93.8%) and 199 (76.8%) were polymorphic, respectively. The SSR makers alleles polymorphism information content(PIC) ranged from 0.665 4 to 0.798 3,with an average of 0.755 6. The effective number of alleles for each locus (Ne) varied from 1.159 2 to 1.971 1,with an average of 1.751 4.The Shannon-Weaver diversity index (H) ranged from 0.2643 to 0.8362 and averaged 0.596 9.The SRAP makers alleles polymorphism information content(PIC) ranged from 0.354 6 to 0.932 1, with an average of 0.743. The effective number of alleles for each locus (Ne) varied from 1.102 4 to 1.992 8, with an average of 1.672 4. The Shannon-Weaver diversity index (H) ranged from 0.168 2 to 0.691 4 and averaged 0.555 6. The genetic similarity coefficient ranged from 0.46 to 0.95. The 35 materials could be clustered into 4 groups at the genetic similarity coefficient 0.70. Accessions of the same origin were in more than one group, it indicating that abundant genetic diversity existed in the hulled oat materials from Chifeng. The results indicated that the work of hulled oat resources collection, reservation, identification and utilization in Chifeng should be strengthened.