中华实验和临床感染病杂志（电子版）
中華實驗和臨床感染病雜誌（電子版）
중화실험화림상감염병잡지（전자판）
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL INFECTIOUS DISEASES(ELECTRONIC VERSION)
2015年
4期
506-509
,共4页
薛万华%刘静%牟云清%李仁哲
薛萬華%劉靜%牟雲清%李仁哲
설만화%류정%모운청%리인철
多重耐药%鲍曼不动杆菌%氨基糖苷类耐药基因%标本分布
多重耐藥%鮑曼不動桿菌%氨基糖苷類耐藥基因%標本分佈
다중내약%포만불동간균%안기당감류내약기인%표본분포
Multidrug-resistant%Acinetobacter baumannii%Aminoglycoside resistance gene%Distribution of specimens
目的：研究临床分离的多重耐药鲍曼不动杆菌氨基糖苷类耐药基因的流行情况及标本的来源，为临床控制感染提供依据。方法采用VITEK-2全自动微生物仪对2013年10月至2013年12月济宁市第一人民医院临床分离的15株多重耐药鲍曼不动杆菌进行细菌鉴定和药敏试验，部分抗菌药物的敏感性采用纸片扩散法。PCR法检测氨基糖苷类耐药基因，对部分阳性基因进行测序。结果15株多重耐药鲍曼不动杆菌中，ant（3"）-Ⅰ基因73.3%（11/15）阳性，aac（6'）-Ⅰ基因66.7%（10/15）阳性，armA基因93.3%（14/15）阳性。93.3%（14/15）的标本分布在ICU病房，6.7%（1/15）的标本分布在神经外科病房。所有标本来源于痰液。结论该院临床分离的多重耐药鲍曼不动杆菌对氨基糖苷类抗菌药物耐药与同时携带多种氨基糖苷类耐药基因有关。应重点控制和预防ICU重症监护病房多重耐药鲍曼不动杆菌引起的院内感染。
目的：研究臨床分離的多重耐藥鮑曼不動桿菌氨基糖苷類耐藥基因的流行情況及標本的來源，為臨床控製感染提供依據。方法採用VITEK-2全自動微生物儀對2013年10月至2013年12月濟寧市第一人民醫院臨床分離的15株多重耐藥鮑曼不動桿菌進行細菌鑒定和藥敏試驗，部分抗菌藥物的敏感性採用紙片擴散法。PCR法檢測氨基糖苷類耐藥基因，對部分暘性基因進行測序。結果15株多重耐藥鮑曼不動桿菌中，ant（3"）-Ⅰ基因73.3%（11/15）暘性，aac（6'）-Ⅰ基因66.7%（10/15）暘性，armA基因93.3%（14/15）暘性。93.3%（14/15）的標本分佈在ICU病房，6.7%（1/15）的標本分佈在神經外科病房。所有標本來源于痰液。結論該院臨床分離的多重耐藥鮑曼不動桿菌對氨基糖苷類抗菌藥物耐藥與同時攜帶多種氨基糖苷類耐藥基因有關。應重點控製和預防ICU重癥鑑護病房多重耐藥鮑曼不動桿菌引起的院內感染。
목적：연구림상분리적다중내약포만불동간균안기당감류내약기인적류행정황급표본적래원，위림상공제감염제공의거。방법채용VITEK-2전자동미생물의대2013년10월지2013년12월제저시제일인민의원림상분리적15주다중내약포만불동간균진행세균감정화약민시험，부분항균약물적민감성채용지편확산법。PCR법검측안기당감류내약기인，대부분양성기인진행측서。결과15주다중내약포만불동간균중，ant（3"）-Ⅰ기인73.3%（11/15）양성，aac（6'）-Ⅰ기인66.7%（10/15）양성，armA기인93.3%（14/15）양성。93.3%（14/15）적표본분포재ICU병방，6.7%（1/15）적표본분포재신경외과병방。소유표본래원우담액。결론해원림상분리적다중내약포만불동간균대안기당감류항균약물내약여동시휴대다충안기당감류내약기인유관。응중점공제화예방ICU중증감호병방다중내약포만불동간균인기적원내감염。
Objective To investigate the prevalence and source of specimen of aminoglycoside resistance gene on clinical isolated multidrug-resistant Acinetobacter baumannii, and to guide the clinical infection control. Methods Identiifcation of bacteria and susceptibility test were conducted on 15 stains of multidrug-resistant Acinetobacter baumannii isolated from October 2013 to December 2013 in First People’ Hospital of Jining, and partial antimicrobial drug susceptibility were detected by the disk diffusion method. Aminoglycoside resistance gene was detected by PCR and the positive gene was partly sequenced. Results Among the 15 stains of multidrug-resistant Acinetobacter baumannii, ant(3")-Ⅰgene showed 73.3%(11/15) positive, aac(6')-Ⅰgene with 66.7%(10/15) positive and armA gene with 93.3%(14/15) positive. There were 93.3%(14/15) of specimens distributed in ICU, while 6.7%(1/15) in neurosurgery ward. All specimens were from sputum. Conclusions Antimicrobial resistance to aminoglycosides of multidrug-resistant Acinetobacter baumannii isolated in the hospital was related to meanwhile carrying multiple aminoglycoside resistance gene. Nosocomial infection caused by multidrug-resistant Acinetobacter baumannii in ICU should be vigorously controlled and prevented.
