中华实验和临床感染病杂志（电子版）
中華實驗和臨床感染病雜誌（電子版）
중화실험화림상감염병잡지（전자판）
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL INFECTIOUS DISEASES(ELECTRONIC VERSION)
2015年
4期
494-497
,共4页
吴晓光%张立群%高孟秋%马丽萍%陈红梅%宋艳华%刘荣梅%黄麦玲%李雪莲%谢莉
吳曉光%張立群%高孟鞦%馬麗萍%陳紅梅%宋豔華%劉榮梅%黃麥玲%李雪蓮%謝莉
오효광%장립군%고맹추%마려평%진홍매%송염화%류영매%황맥령%리설련%사리
结核病%结核潜伏感染%miR-146a
結覈病%結覈潛伏感染%miR-146a
결핵병%결핵잠복감염%miR-146a
Tuberculosis%Latent tuberculosis infection (LTBI)%miR-146a
目的：研究miR-146a在结核病患者外周血单个核细胞中的表达水平和临床意义。方法采用Trizonl一步法从结核病患者、结核病潜伏感染者和健康对照外周血单个核细胞提取总RNA，荧光定量PCR测定miR-146a的表达。同时使用荧光定量PCR测定样本中肿瘤坏死因子受体相关因子6、白细胞介素受体相关激酶1和肿瘤坏死因子的表达。结果肺结核患者外周血单核细胞miR-146a表达较结核潜伏感染和健康对照者显著下降，而潜伏感染者miR-146a表达水平（4.49±0.61）与对照组（4.57±0.25）没有差异。同时，miR-146a下游靶基因TRAF6、IRAK-1以及TNF-α表达在活动性肺结核患者中较结核潜伏感染和健康对照者增加。结论与健康对照和潜伏感染者相比，miR-146a在结核病患者外周血单个核细胞中表达显著下降，推测miR-146a有可能成为鉴别活动性结核和结核潜伏感染新的标记物。
目的：研究miR-146a在結覈病患者外週血單箇覈細胞中的錶達水平和臨床意義。方法採用Trizonl一步法從結覈病患者、結覈病潛伏感染者和健康對照外週血單箇覈細胞提取總RNA，熒光定量PCR測定miR-146a的錶達。同時使用熒光定量PCR測定樣本中腫瘤壞死因子受體相關因子6、白細胞介素受體相關激酶1和腫瘤壞死因子的錶達。結果肺結覈患者外週血單覈細胞miR-146a錶達較結覈潛伏感染和健康對照者顯著下降，而潛伏感染者miR-146a錶達水平（4.49±0.61）與對照組（4.57±0.25）沒有差異。同時，miR-146a下遊靶基因TRAF6、IRAK-1以及TNF-α錶達在活動性肺結覈患者中較結覈潛伏感染和健康對照者增加。結論與健康對照和潛伏感染者相比，miR-146a在結覈病患者外週血單箇覈細胞中錶達顯著下降，推測miR-146a有可能成為鑒彆活動性結覈和結覈潛伏感染新的標記物。
목적：연구miR-146a재결핵병환자외주혈단개핵세포중적표체수평화림상의의。방법채용Trizonl일보법종결핵병환자、결핵병잠복감염자화건강대조외주혈단개핵세포제취총RNA，형광정량PCR측정miR-146a적표체。동시사용형광정량PCR측정양본중종류배사인자수체상관인자6、백세포개소수체상관격매1화종류배사인자적표체。결과폐결핵환자외주혈단핵세포miR-146a표체교결핵잠복감염화건강대조자현저하강，이잠복감염자miR-146a표체수평（4.49±0.61）여대조조（4.57±0.25）몰유차이。동시，miR-146a하유파기인TRAF6、IRAK-1이급TNF-α표체재활동성폐결핵환자중교결핵잠복감염화건강대조자증가。결론여건강대조화잠복감염자상비，miR-146a재결핵병환자외주혈단개핵세포중표체현저하강，추측miR-146a유가능성위감별활동성결핵화결핵잠복감염신적표기물。
Objective To study the expression of miR-146a in tuberculosis (TB) patients and latent tuberculosis infection (LTBI). Methods Total RNA was isolated from peripheral blood mononuclear cells (PBMCs) obtained from patients with TB, and healthy and LTBI individuals, and the expression of miR-146a was analyzed by quantitative real-time PCR. In addition, two targets of miR-146a, namely tumor necrosis factor receptor-associated factor 6 (TRAF6) and IL-1 receptor-associated kinase 1 (IRAK-1) and tumor necrosis factor-α(TNF-α) were also analyzed by quantitative real-time PCR. Results PBMCs of patients with TB exhibited signiifcantly decreased in miR-146a expression, whereas miR-146a expression was not significantly different compared with healthy control individuals (4.57 ± 0.25) and LTBI (4.49 ± 0.61). TRAF6, IRAK-1 and TNF-α were increased expression between patients with TB and LTBI and control individuals, implicating that miR-146a plays a key role in the active tuberculosis. Conclusions Compare with health control and LTBI, the miR-146a expression level was down-regulated in TB patients. This indicated that miR-146a may be a potentially useful as a biomarker for diagnosis of TB and LTBI.
