CAJ | 학술논문

目的：观察不同免疫状态下慢性乙型肝炎病毒（HBV）感染者外周血调节性B细胞（Breg细胞）比例及血清白细胞介素-10（IL-10）和干扰素诱导蛋白-10（IP-10）水平特点。方法采集63例不同免疫状态下慢性HBV感染者和16例健康个体的外周血，采用流式细胞术检测其外周血Breg细胞（CD24hiCD38hi B细胞）比例，采用Luminex技术检测其血清IL-10和IP-10水平。结果免疫活化组外周血Breg细胞比例高于免疫耐受组和健康对照组[（7.89±3.37）% vs （4.77±2.42）%， F=9.27，P=0.010；（7.89±3.37）%vs （3.83±2.14）%，F=16.55，P＜0.001]，免疫耐受组成熟B细胞（CD24intCD38int B细胞）比例低于健康对照组[（43.40±10.74）% vs （54.56±10.72）%， F=7.39，P=0.02]。免疫活化组血清IL-10和IP-10水平高于免疫耐受组、非活动HBV携带组及健康对照组[（22.53±24.81） pg/ml vs （0.69±1.34）pg/ml，（22.53±24.81） pg/ml vs （0.31±1.12） pg/ml，（22.53±24.81） pg/ml vs （0.003±0.009）pg/ml，P均＜0.001；（2540.19±1870.73）pg/ml vs （720.52±285.73）pg/ml，（2540.19±1870.73）pg/ml vs （567.38±208.72）pg/ml，（2540.19±1870.73）pg/ml vs （624.80±274.45）pg/ml，P均＜0.001]。慢性HBV感染者外周血Breg细胞比例与血清IL-10水平、ALT水平呈正相关关系（r=0.282，P=0.025；r=0.305，P=0.026），免疫活化期感染者血清IL-10、IP-10水平与ALT水平呈正相关（r=0.715，P＜0.001；r=0.653，P＜0.001）。结论免疫活化期的慢性HBV感染者Breg细胞比例升高，且其外周血IL-10、IP-10也显著升高。慢性HBV感染者外周血Breg细胞比例与血清IL-10、ALT水平正相关，免疫活化期感染者血清IL-10、IP-10水平与ALT水平正相关。
목적：관찰불동면역상태하만성을형간염병독（HBV）감염자외주혈조절성B세포（Breg세포）비례급혈청백세포개소-10（IL-10）화간우소유도단백-10（IP-10）수평특점。방법채집63례불동면역상태하만성HBV감염자화16례건강개체적외주혈，채용류식세포술검측기외주혈Breg세포（CD24hiCD38hi B세포）비례，채용Luminex기술검측기혈청IL-10화IP-10수평。결과면역활화조외주혈Breg세포비례고우면역내수조화건강대조조[（7.89±3.37）% vs （4.77±2.42）%， F=9.27，P=0.010；（7.89±3.37）%vs （3.83±2.14）%，F=16.55，P＜0.001]，면역내수조성숙B세포（CD24intCD38int B세포）비례저우건강대조조[（43.40±10.74）% vs （54.56±10.72）%， F=7.39，P=0.02]。면역활화조혈청IL-10화IP-10수평고우면역내수조、비활동HBV휴대조급건강대조조[（22.53±24.81） pg/ml vs （0.69±1.34）pg/ml，（22.53±24.81） pg/ml vs （0.31±1.12） pg/ml，（22.53±24.81） pg/ml vs （0.003±0.009）pg/ml，P균＜0.001；（2540.19±1870.73）pg/ml vs （720.52±285.73）pg/ml，（2540.19±1870.73）pg/ml vs （567.38±208.72）pg/ml，（2540.19±1870.73）pg/ml vs （624.80±274.45）pg/ml，P균＜0.001]。만성HBV감염자외주혈Breg세포비례여혈청IL-10수평、ALT수평정정상관관계（r=0.282，P=0.025；r=0.305，P=0.026），면역활화기감염자혈청IL-10、IP-10수평여ALT수평정정상관（r=0.715，P＜0.001；r=0.653，P＜0.001）。결론면역활화기적만성HBV감염자Breg세포비례승고，차기외주혈IL-10、IP-10야현저승고。만성HBV감염자외주혈Breg세포비례여혈청IL-10、ALT수평정상관，면역활화기감염자혈청IL-10、IP-10수평여ALT수평정상관。
Objective To investigate the frequencies of regulatory B cells (Bregs) in the peripheral blood and the levels of serum interleukin-10 (IL-10) and interferon-γ-induced protein 10 (IP-10) in different immune phases of chronic hepatitis B virus (HBV) infection. Methods Total of sixty-three patients with chronic HBV infection, while 16 healthy controls were enrolled. The frequencies of Bregs (CD24hiCD38hi B cells) in the peripheral blood were measured by lfow cytometry. IL-10 and IP-10 levels were determined with the Human Magnetic Cytokine/Chemokine Bead Panel on the MAGPIX instrument. Results Compared to immune tolerant phase (IT) and healthy controls (HC), the frequencies of Bregs were signiifcantly elevated in the immune reactive phase (IA) [ (7.89 ± 3.37)%vs (4.77 ± 2.42%), F=9.27, P=0.010;(7.89 ± 3.37)%vs (3.83 ± 2.14)%, F=16.55, P<0.001 ]. The frequencies of mature B cells (CD24intCD38int B cells) were signiifcantly, P=0.02]. Serum IL-10 and IP-10 levels were also elevated signiifcantly in IA group as compared with IT, inactive HBV carrier state (IC) and HC group [(22.53 ± 24.81) pg/ml vs (0.69 ± 1.34) pg/ml, (22.53 ± 24.81) pg/ml vs (0.31 ± 1.12) pg/ml, (22.53 ± 24.81) pg/ml vs (0.003 ± 0.009) pg/ml, P all < 0.001;(2 540.19 ± 1 870.73) pg/ml vs (720.52 ± 285.73) pg/ml, (2 540.19 ± 1 870.73) pg/ml vs (567.38 ± 208.72) pg/ml, (2 540.19 ± 1 870.73) pg/ml vs (624.80 ± 274.45) pg/ml, P all<0.001]. The frequencies of Bregs positively correlated with IL-10 and ALT levels (r = 0.282, P = 0.025; r = 0.305, P = 0.026) in chronic HBV infection. In addition, IL-10 and IP-10 levels were also positively correlated with ALT levels (r = 0.715, P < 0.001; r = 0.653, P < 0.001) in immune reactive phase (IA). Conclusions The frequencies of Bregs, IL-10 and IP-10 levels were elevated significantly in immune reactive phase (IA) of chronic HBV infection. The frequencies of Bregs positively correlated with IL-10 and ALT levels in chronic HBV infection. IL-10 and IP-10 levels were also positively correlated with ALT levels in immune reactive phase (IA) of chronic HBV infection.