CAJ | 학술논문

目的：研究补肾固筋方对骨关节炎（ knee osteoarthritis，KOA） TNF-α、iNOS的作用调节机制。方法0.3岁雄性山羊72只，随机分为正常组、模型组、西药组、中药组，每组18只，改良Hulth造模，取血清及股骨内髁关节软骨滑膜及软骨下骨， ELISA法检测TNF-α、iNOS。结果西药组关节间隙狭窄，介于模型组和中药组之间，关节软骨面外观粗糙骨赘增生；中药组关节间隙稍窄，关节面稍粗糙伴轻微骨赘；镜下模型组软骨细胞呈成簇现象增生，中药组软骨细胞大小均匀且排列整齐；TNF-α、iNOS含量模型组中比正常组增高（P＜0.01）。两个给药组比模型组明显降低（P＜0.01），两给药组之间无差异统计学意义（ P＞0.05）。结论补肾固筋方有效降低骨关节炎TNF-α、iNOS含量，促进软骨修复，提供实验依据。
목적：연구보신고근방대골관절염（ knee osteoarthritis，KOA） TNF-α、iNOS적작용조절궤제。방법0.3세웅성산양72지，수궤분위정상조、모형조、서약조、중약조，매조18지，개량Hulth조모，취혈청급고골내과관절연골활막급연골하골， ELISA법검측TNF-α、iNOS。결과서약조관절간극협착，개우모형조화중약조지간，관절연골면외관조조골췌증생；중약조관절간극초착，관절면초조조반경미골췌；경하모형조연골세포정성족현상증생，중약조연골세포대소균균차배렬정제；TNF-α、iNOS함량모형조중비정상조증고（P＜0.01）。량개급약조비모형조명현강저（P＜0.01），량급약조지간무차이통계학의의（ P＞0.05）。결론보신고근방유효강저골관절염TNF-α、iNOS함량，촉진연골수복，제공실험의거。
Objective To explore the regulation effect of tonifying kidney and consolidating tendon prescription on tumor necrosis factor-alpha ( TNF-α) and inducible nitric oxide synthase ( iNOS) in knee osteoarthritis ( KOA) goats.Methods A total of 72 3-month-old healthy male goats were randomly divided into normal group, model group, Western medicine group, and TCM groups, with 18 goats in each group.The model was established using modified Hulth method.Serum and the cartilage, synovial membrane, and the subchondral bone of the femur and the medial malleolus were collected.TNF-αand iNOS were detected using ELISA method.Results The joint space narrowed in Western medicine group, and it was between the model group and the TCM group.The articular surface became roughness and the osteophyte developed.The joint space slightly narrowed in the TCM group, and the articular surface was slightly rough with mild osteophytes.The chondrocytes proliferated in a cluster manner with even size and good order under the microscope.The expression of TNF-αand iNOS was significantly higher in the model group than that in the normal group ( P <0.01 ) .It was significantly lower in both medicine groups than in the model group ( P <0.01 ) .The difference between the two medicine groups was not significant ( P>0.05 ) .Conclusion The tonifying kidney and consolidating tendon prescription effectively decreases the content of TNF-αand iNOS in KOA and promotes the repair of the cartilage.This provides experimental evidence for the clinical treatment.