CAJ | 학술논문

本研究试图通过检测大麻素（CB）的两种主要水解酶———N-乙酰基乙醇胺水解酸性酰胺酶（NAAA）、脂肪酸酰胺水解酶（ FAAH）在鼠累积损伤性椎间盘炎症反应过程中的表达来初步揭示其中大麻素的参与过程及作用机制。方法清洁级Wistar大鼠随即分成实验组与对照组，根据Jill A．Ulrich制作方法准备实验组，以切皮后即缝合为对照组，在术后第3、7、10天，实验组与对照组每次各取6只，取出相应椎间盘，进行形态学观察，应用Real Time-PCR技术检测FAAH、NAAA的表达水平。结果与对照组相比，实验10d组可见刺伤所致纤维环断裂，髓核内出血。在椎间盘累积性损伤7d时， NAAA和FAAH表达含量分别约为对照组的3倍，并形成高峰；而在损伤10 d后，其表达量呈减少趋势。结论作为水解酶的底物CB类物质在第7天时候表达含量呈低谷，而随着时间的延长逐渐增高。 CB类物质参与了炎症反应的发展过程，从而抑制骨骼椎间盘的退变。
본연구시도통과검측대마소（CB）적량충주요수해매———N-을선기을순알수해산성선알매（NAAA）、지방산선알수해매（ FAAH）재서루적손상성추간반염증반응과정중적표체래초보게시기중대마소적삼여과정급작용궤제。방법청길급Wistar대서수즉분성실험조여대조조，근거Jill A．Ulrich제작방법준비실험조，이절피후즉봉합위대조조，재술후제3、7、10천，실험조여대조조매차각취6지，취출상응추간반，진행형태학관찰，응용Real Time-PCR기술검측FAAH、NAAA적표체수평。결과여대조조상비，실험10d조가견자상소치섬유배단렬，수핵내출혈。재추간반루적성손상7d시， NAAA화FAAH표체함량분별약위대조조적3배，병형성고봉；이재손상10 d후，기표체량정감소추세。결론작위수해매적저물CB류물질재제7천시후표체함량정저곡，이수착시간적연장축점증고。 CB류물질삼여료염증반응적발전과정，종이억제골격추간반적퇴변。
Objective To reveal the function of CB during disc inflammation due to cumulative damage through detecting the expression level of the key hydrolyses of CB FAAH and NAAA.Methods Wister rats were randomly divided into two groups. Rats in the experimental group were prepared according to Jill A.Ulrich’ s method.Control group followed skin cut and close method.Six rats were collected from each group at the 3nd, 7th, and 10th day after the operation.The corresponding intervertebral disc was removed.Morphology was observed.The expression levels of FAAH and NAAA were detected using real-time PCR method.Results Compared to the control group, the rupture and nucleus bleeding were observed in 10 days in experimental group.At the 7th day after cumulative damage to the disc, NAAA and FAAH expression levels reached a peak at approximately 3 times over the control group.The expression decreased after 10 days of the injury.Conclusion As a substrate for the hydrolysis enzyme, CB expresses at a bottom level at the 7th day postoperatively, but it gradually increases over the later time.CB is involved in the inflammative reaction and leads to the inhibition of th disc degeneration.