中国骨质疏松杂志
中國骨質疏鬆雜誌
중국골질소송잡지
CHINESE JOURNAL OF OSTEOPOROSIS
2015年
6期
674-678,711
,共6页
邓伟民%魏秋实%郭桢%谭新%黄思敏%王俊玲%邵玉
鄧偉民%魏鞦實%郭楨%譚新%黃思敏%王俊玲%邵玉
산위민%위추실%곽정%담신%황사민%왕준령%소옥
绝经后骨质疏松症%类固醇受体辅助激活因子3%过氧化物酶体增殖物活化受体γ协同刺激因子-1α
絕經後骨質疏鬆癥%類固醇受體輔助激活因子3%過氧化物酶體增殖物活化受體γ協同刺激因子-1α
절경후골질소송증%류고순수체보조격활인자3%과양화물매체증식물활화수체γ협동자격인자-1α
Postmenopausal osteoporosis%SRC-3%PGC-1α
目的:探讨绝经后骨质疏松症患者骨组织中类固醇受体辅助激活因子3(steroid receptor coactivator-3, SRC-3)和过氧化物酶体增殖物活化受体γ协同刺激因子-1α( peroxisome proliferator-activated receptor gamma coactivator 1α, PGC-1α)的表达及意义。方法2012年6月~2013年9月,选择于广州军区广州总医院关节外科住院的行全髋关节置换患者20例,术前双能X线(DXA)骨密度仪测量患者腰椎1~4(L1-4)骨密度,根据WHO颁布的诊断标准,分为试验组(腰椎BMD T评分<-2.5,10例)和对照组( T评分>-1.0,10例),术中取一侧开路器切除的粗隆部位松质骨。荧光实时定量RT-PCR和Western blotting分别检测骨组织中SRC-3和PGC-1α等mRNA和蛋白的表达。结果与对照组相比,试验组骨组织中SRC-3, PGC-1α, CBP/P300, P/CAF mRNA表达水平均下降(P值分别为<0.001,0.036,0.003,0.027),OPN mRNA表达水平升高(P=0.004)。Osteocalin mRNA表达在试验组和对照组中无统计学差异( P>0.05)。试验组SRC-3和PGC-1α蛋白相对表达量较对照组明显下调,分别下调了(53.23±0.55)%( P=0.037)和(72.17±0.64)%( P=0.003)。结论 SRC-3和PGC-1α可能参与绝经后骨质疏松症发病的病理过程。
目的:探討絕經後骨質疏鬆癥患者骨組織中類固醇受體輔助激活因子3(steroid receptor coactivator-3, SRC-3)和過氧化物酶體增殖物活化受體γ協同刺激因子-1α( peroxisome proliferator-activated receptor gamma coactivator 1α, PGC-1α)的錶達及意義。方法2012年6月~2013年9月,選擇于廣州軍區廣州總醫院關節外科住院的行全髖關節置換患者20例,術前雙能X線(DXA)骨密度儀測量患者腰椎1~4(L1-4)骨密度,根據WHO頒佈的診斷標準,分為試驗組(腰椎BMD T評分<-2.5,10例)和對照組( T評分>-1.0,10例),術中取一側開路器切除的粗隆部位鬆質骨。熒光實時定量RT-PCR和Western blotting分彆檢測骨組織中SRC-3和PGC-1α等mRNA和蛋白的錶達。結果與對照組相比,試驗組骨組織中SRC-3, PGC-1α, CBP/P300, P/CAF mRNA錶達水平均下降(P值分彆為<0.001,0.036,0.003,0.027),OPN mRNA錶達水平升高(P=0.004)。Osteocalin mRNA錶達在試驗組和對照組中無統計學差異( P>0.05)。試驗組SRC-3和PGC-1α蛋白相對錶達量較對照組明顯下調,分彆下調瞭(53.23±0.55)%( P=0.037)和(72.17±0.64)%( P=0.003)。結論 SRC-3和PGC-1α可能參與絕經後骨質疏鬆癥髮病的病理過程。
목적:탐토절경후골질소송증환자골조직중류고순수체보조격활인자3(steroid receptor coactivator-3, SRC-3)화과양화물매체증식물활화수체γ협동자격인자-1α( peroxisome proliferator-activated receptor gamma coactivator 1α, PGC-1α)적표체급의의。방법2012년6월~2013년9월,선택우엄주군구엄주총의원관절외과주원적행전관관절치환환자20례,술전쌍능X선(DXA)골밀도의측량환자요추1~4(L1-4)골밀도,근거WHO반포적진단표준,분위시험조(요추BMD T평분<-2.5,10례)화대조조( T평분>-1.0,10례),술중취일측개로기절제적조륭부위송질골。형광실시정량RT-PCR화Western blotting분별검측골조직중SRC-3화PGC-1α등mRNA화단백적표체。결과여대조조상비,시험조골조직중SRC-3, PGC-1α, CBP/P300, P/CAF mRNA표체수평균하강(P치분별위<0.001,0.036,0.003,0.027),OPN mRNA표체수평승고(P=0.004)。Osteocalin mRNA표체재시험조화대조조중무통계학차이( P>0.05)。시험조SRC-3화PGC-1α단백상대표체량교대조조명현하조,분별하조료(53.23±0.55)%( P=0.037)화(72.17±0.64)%( P=0.003)。결론 SRC-3화PGC-1α가능삼여절경후골질소송증발병적병리과정。
Objective To explore the expression and significance of steroid receptor coactivator-3 ( SRC-3 ) and peroxisome proliferator-activated receptor gamma coactivator 1α( PGC-1α) in the bone in patients with postmenopausal osteoporosis.Methods A total of 20 cases of postmenopausal women with osteoporosis, who were in the inpatient at orthopedic department in General Hospital of Guangzhou Military Command of PLA for total hip arthoplasty from June 2012 to September 2013, were selected.BMD of the lumbar spine ( L1-4 ) was measured using DEXA before the operation.According to the WHO criteria.the subjects were divided to the experimental group (10 cases, T score <-2.5) and control group (10 cases, T score >-1.0) .Cancellous bone samples were collected from the greater trochanter which was cut by open circuit device.The mRNA and protein relative expressions of SRC-3 and PGC-1αwere determined using quantitative PCR and Western blotting, respectively.Results Compared with control group, the relative expression of SRC-3, PGC-1α, CBP/P300, and P/CAF mRNA in experimental group decreased ( P<0.001, 0.036, 0.003, and 0.027, respectively), but the relative expression of OPN mRNA increased ( P =0.004).No statistically significance was found in the relative expression of osteocalcin mRNA between the two groups.Compared with control group, the protein relative expression of SRC-3 and PGC-1αreduced significantly (53.23 ±0.55%, P=0.037;72.17 ±0.64%, P=0.003, respectively) .Conclusion SRC-3 and PGC-1αmay be involved in the pathogenesis of postmenopausal osteoporosis.