中国骨质疏松杂志
中國骨質疏鬆雜誌
중국골질소송잡지
CHINESE JOURNAL OF OSTEOPOROSIS
2015年
6期
669-673,690
,共6页
贺行文%陶同善%柳维%周贤挺%屠凯凯%杨雷
賀行文%陶同善%柳維%週賢挺%屠凱凱%楊雷
하행문%도동선%류유%주현정%도개개%양뢰
骨质疏松%骨缺损%PTH%Micro-CT
骨質疏鬆%骨缺損%PTH%Micro-CT
골질소송%골결손%PTH%Micro-CT
Osteoporosis%Bone defect%PTH%Micro-CT
目的：探索特立帕肽（ PTH）对去势大鼠骨缺损修复的影响。方法选取SPF级3月龄雌性SD大鼠20只，行双侧卵巢切除术，饲养3月后骨质疏松动物模型成功建立，然后于大鼠两侧股骨髁上行直径3 mm贯通性骨缺损手术，术后随机分为对照组和PTH治疗组，PTH治疗组于缺损手术1 w后，每周给予背部皮下注射特立帕肽5次（30μg／kg），空白对照组注射等量的生理盐水。术后4，8 w进行组织病理学观察，Micro-CT分析检测。结果术后4、8 w，PTH治疗组和对照组对比发现：PTH治疗组股骨骨密度均高于同时间段的对照组，缺损区Micro-CT扫描显示PTH治疗组较对照组有更为明显的新生骨组织形成，骨缺损术后4、8w时，两组大鼠股骨干骺端骨缺损区域骨小梁体积分数（BV／TV）、骨小梁厚度（Tb．Th）、骨小梁数量（Tb．N）均逐渐增加，骨小梁分离度（ Tb．Sp）降低， PTH治疗组BV／TV、Tb．Th及Tb．N均较同时期的空白对照组增高，骨小梁分离度（Tb．Sp）则低于空白对照组，差异有统计学意义（P＜0.05）。缺损术后8w时PTH治疗组骨缺损区域可见大量排列致密且形态粗大的骨小梁，骨缺损区域基本愈合，而对照组缺损区域愈合欠佳。结论 PTH可较好地促进骨质疏松性大鼠股骨干骺端骨缺损的修复。
目的：探索特立帕肽（ PTH）對去勢大鼠骨缺損脩複的影響。方法選取SPF級3月齡雌性SD大鼠20隻，行雙側卵巢切除術，飼養3月後骨質疏鬆動物模型成功建立，然後于大鼠兩側股骨髁上行直徑3 mm貫通性骨缺損手術，術後隨機分為對照組和PTH治療組，PTH治療組于缺損手術1 w後，每週給予揹部皮下註射特立帕肽5次（30μg／kg），空白對照組註射等量的生理鹽水。術後4，8 w進行組織病理學觀察，Micro-CT分析檢測。結果術後4、8 w，PTH治療組和對照組對比髮現：PTH治療組股骨骨密度均高于同時間段的對照組，缺損區Micro-CT掃描顯示PTH治療組較對照組有更為明顯的新生骨組織形成，骨缺損術後4、8w時，兩組大鼠股骨榦骺耑骨缺損區域骨小樑體積分數（BV／TV）、骨小樑厚度（Tb．Th）、骨小樑數量（Tb．N）均逐漸增加，骨小樑分離度（ Tb．Sp）降低， PTH治療組BV／TV、Tb．Th及Tb．N均較同時期的空白對照組增高，骨小樑分離度（Tb．Sp）則低于空白對照組，差異有統計學意義（P＜0.05）。缺損術後8w時PTH治療組骨缺損區域可見大量排列緻密且形態粗大的骨小樑，骨缺損區域基本愈閤，而對照組缺損區域愈閤欠佳。結論 PTH可較好地促進骨質疏鬆性大鼠股骨榦骺耑骨缺損的脩複。
목적：탐색특립파태（ PTH）대거세대서골결손수복적영향。방법선취SPF급3월령자성SD대서20지，행쌍측란소절제술，사양3월후골질소송동물모형성공건립，연후우대서량측고골과상행직경3 mm관통성골결손수술，술후수궤분위대조조화PTH치료조，PTH치료조우결손수술1 w후，매주급여배부피하주사특립파태5차（30μg／kg），공백대조조주사등량적생리염수。술후4，8 w진행조직병이학관찰，Micro-CT분석검측。결과술후4、8 w，PTH치료조화대조조대비발현：PTH치료조고골골밀도균고우동시간단적대조조，결손구Micro-CT소묘현시PTH치료조교대조조유경위명현적신생골조직형성，골결손술후4、8w시，량조대서고골간후단골결손구역골소량체적분수（BV／TV）、골소량후도（Tb．Th）、골소량수량（Tb．N）균축점증가，골소량분리도（ Tb．Sp）강저， PTH치료조BV／TV、Tb．Th급Tb．N균교동시기적공백대조조증고，골소량분리도（Tb．Sp）칙저우공백대조조，차이유통계학의의（P＜0.05）。결손술후8w시PTH치료조골결손구역가견대량배렬치밀차형태조대적골소량，골결손구역기본유합，이대조조결손구역유합흠가。결론 PTH가교호지촉진골질소송성대서고골간후단골결손적수복。
Objective To explore the effect of teriparatide ( PTH) on bone defect healing in ovariectomized rats.Methods Twenty SPF grade 3-month-old SD rats were selected and ovariectomized, and fed for 3 months to establish the osteoporosis model. Then a 3mm femur defect drilling was created in bilateral femoral metaphysis of the rats.After the operation, rats were divided into control group and PTH-treated group.After one week, rats in PTH-treated group received subcutaneous PTH injection five times (30 ug/kg) , while rats in control group received saline injection.Histological studies and mirco-CT analysis were performed at 4 and 8 weeks after the surgery.Results Compared to that in control group, bone mineral density increased significantly in PTH-treated group after 4 and 8 weeks of operation.Micro-CT images of the defect showed that total bone volume/total tissue volume, cortical wall thickness, trabecular thickness, trabecular number increased significantly, and trabecular space decreased significantly in PTH-treated group compared to those in control group ( P<0.05) .In 8 weeks, large tense-arrayed and thick trabeculae could be seen in the defect area, and the defect was almost healed in PTH-treated group, while in control group the defect was not well healed.Conclusion PTH can promote femoral metaphases bone defect repair in osteoporotic rats.
