CAJ | 학술논문

目的：低强度脉冲超声具有促进成骨细胞增殖和矿化的作用，本实验在以往细胞实验的基础上，探究一定参数的超声对骨质疏松症小鼠模型的作用。方法采用8周龄SPF级BalB／C雌性小鼠，对其实施双侧卵巢切除术建立小鼠骨质疏松症模型，并确定建模成功。实验设置空白对照组、阳性对照组（雌激素灌胃）和超声实验组。阳性对照组给予含结合雌激素（1.24μg／只）的超纯水溶液灌胃。超声组选用频率为1.5 MHz强度为40 mW／cm2的超声照射脊柱5 min，每周5次，持续8 w后检测脊柱区域骨组织密度和生物力学性能。结果建模后小鼠血清中雌二醇含量降低，并且骨密度明显下降。经过后续干预治疗后，雌激素治疗组和超声组骨密度有一定提高。生物力学测试结果显示雌激素组和超声组较对照组而言，脊柱最大载荷和最大位移均有所提高。实验后对模型小鼠五脏进行病理切片，未见血点，细胞结构完整。结论本实验成功建立了小鼠骨质疏松症模型，低强度脉冲超声能够提高骨质疏松症小鼠模型的骨密度，增加骨强度和骨韧性且该参数的超声具有良好的生物安全性。
목적：저강도맥충초성구유촉진성골세포증식화광화적작용，본실험재이왕세포실험적기출상，탐구일정삼수적초성대골질소송증소서모형적작용。방법채용8주령SPF급BalB／C자성소서，대기실시쌍측란소절제술건립소서골질소송증모형，병학정건모성공。실험설치공백대조조、양성대조조（자격소관위）화초성실험조。양성대조조급여함결합자격소（1.24μg／지）적초순수용액관위。초성조선용빈솔위1.5 MHz강도위40 mW／cm2적초성조사척주5 min，매주5차，지속8 w후검측척주구역골조직밀도화생물역학성능。결과건모후소서혈청중자이순함량강저，병차골밀도명현하강。경과후속간예치료후，자격소치료조화초성조골밀도유일정제고。생물역학측시결과현시자격소조화초성조교대조조이언，척주최대재하화최대위이균유소제고。실험후대모형소서오장진행병리절편，미견혈점，세포결구완정。결론본실험성공건립료소서골질소송증모형，저강도맥충초성능구제고골질소송증소서모형적골밀도，증가골강도화골인성차해삼수적초성구유량호적생물안전성。
Objective Low-intensity pulsed ultrasound ( LIPUS ) promotes osteoblast proliferation and mineralization.This experiment explores the effect of LIPUS on mouse osteoporosis model on the basis of our previous cell experiments.Methods Eight-week-old healthy female BalB/C mice ( SPF grade ) were ovariectomized bilaterally to establish the mouse osteoporosis model.After the models were established, mice were divided into blank control ( CON) , positive control group ( estrogen group, ESG) , and LIPUS group.The mice were gavaged 1.24 μg estrogen containing distilled water each in the ESG.The mice were irradiated on the spine using ultrasound (1.5 MHz, 40 mW/cm2 ) for 5 minutes in the LIPUS group.Each mouse was treated 5 times per week for 8 weeks.Eight weeks later, bone mineral density and biomechanical properties around the irradiated spine were measured.Results Serum estradiol and bone mineral density decreases obviously after establishing the osteoporosis model in mice.After the follow-up intervention treatment, bone mineral density increased in a certain extent in LIUPS and ESG. Biomechanics detection showed that the maximum load and maximum displacement improved in ESG and LIUPS compared with those in CON.No bleed points were observed and the cell structure was intact in organ histological section after the experiments. Conclusion We successfully establish the mouse model of osteoporosis.The results show that low-intensity pulsed ultrasound is a safe method that can improve bone mineral density in osteoporotic mice and enhance bone strength and bone tenacity.