CAJ | 학술논문

为研究植物内生细菌在缓解寄主植物镉污染中的作用,通过离体条件下耐镉活性鉴定,筛选了5株根围细菌和内生细菌,其中内生细菌菌株1JN2在以辣椒和黄瓜作为寄主进行的盆栽试验中能够增强寄主植物对镉的耐受性,与空白对照(不接种菌株、硫酸镉溶液培养)相比,黄瓜和辣椒叶绿素含量分别增加10.85%、20.14%,根系活力分别增加363.32%、525.51%,组织中镉离子浓度分别降低23.34%、40.39%,在5株细菌中对镉胁迫植物综合保护效果最佳。该菌株经形态学和分子学鉴定为枯草芽孢杆菌。扫描电镜检查发现,1JN2在镉离子处理后24 h开始进行菌体自我复原,在48 h出现胞外多糖固化现象。
위연구식물내생세균재완해기주식물력오염중적작용,통과리체조건하내력활성감정,사선료5주근위세균화내생세균,기중내생세균균주1JN2재이랄초화황과작위기주진행적분재시험중능구증강기주식물대력적내수성,여공백대조(불접충균주、류산력용액배양)상비,황과화랄초협록소함량분별증가10.85%、20.14%,근계활력분별증가363.32%、525.51%,조직중력리자농도분별강저23.34%、40.39%,재5주세균중대력협박식물종합보호효과최가。해균주경형태학화분자학감정위고초아포간균。소묘전경검사발현,1JN2재력리자처리후24 h개시진행균체자아복원,재48 h출현포외다당고화현상。
Experiments on in vitro Cd tolerance were conducted on 183 bacteria strains, five of which were selected for their strong performance. Strain 1JN2 outplayed other four strains in improving host plants Cd-tolerance. Compared to blank control(bacterium free and Cd free), pot-cultured cucumber and pepper treated with strain 1JN2 under Cd stress showed greater chlorophyll contents by 10. 85% and 20. 14%, and stronger root vigor by 363. 32% and 525. 51%. The Cd concentrations in 1JN2-treated cucumber and pepper were decreased by 23. 34% and 40. 39%. Morphological and molecu-lar identification revealed that 1JN2 was a strain of Bacillus subtilis. Scanning electron microscopy showed that strain 1JN2 started to heal itself 24 h post Cd stress and solidify extracellular polysaccharide 48 h post Cd stress.