CAJ | 학술논문

目的：检测Toll样受体8、9在尖锐湿疣局部皮损中的表达情况，探讨它们在尖锐湿疣发病中的作用，为尖锐湿疣的免疫学发病机制提供可的理论依据。方法采用免疫组化Max vision（即用型快速免疫组化一步法）法检测50例尖锐湿疣皮损和10例正常皮肤（包皮）中TLR8、TLR9的表达情况，并应用IPP6.0彩色图像分析系统进行图像分析定量研究。结果⑴免疫组化定位分析：TLR8在CA皮损中于基底层及棘细胞层强阳性表达，颗粒层及角质层中等强度表达；TLR8在正常表皮基底层弱表达，且以胞质型为主，其他角质形成细胞层及真皮层不表达。 TLR9在CA皮损中表皮各层均呈强阳性表达，棘细胞层部分呈颗粒状聚集；TLR9在正常表皮的各层均呈强阳性表达，除角质层外。⑵IPP6.0彩色图像分析定量研究：CA皮损中TLR8和TLR9蛋白表达的相对量分别为0.1267±0.05140和0.1604±0.06153，明显高于正常对照组0.0797±0.02805和0.1263±0.01724，差异均有统计学意义（P<0.05）。结论 TLR8、TLR9在尖锐湿疣患者皮损中的表达较正常对照组明显增强，且集中分布于基底层和棘细胞层。 TLR8、TLR9可能是HPV的识别受体，参与机体局部皮损抗HPV免疫应答。
목적：검측Toll양수체8、9재첨예습우국부피손중적표체정황，탐토타문재첨예습우발병중적작용，위첨예습우적면역학발병궤제제공가적이론의거。방법채용면역조화Max vision（즉용형쾌속면역조화일보법）법검측50례첨예습우피손화10례정상피부（포피）중TLR8、TLR9적표체정황，병응용IPP6.0채색도상분석계통진행도상분석정량연구。결과⑴면역조화정위분석：TLR8재CA피손중우기저층급극세포층강양성표체，과립층급각질층중등강도표체；TLR8재정상표피기저층약표체，차이포질형위주，기타각질형성세포층급진피층불표체。 TLR9재CA피손중표피각층균정강양성표체，극세포층부분정과립상취집；TLR9재정상표피적각층균정강양성표체，제각질층외。⑵IPP6.0채색도상분석정량연구：CA피손중TLR8화TLR9단백표체적상대량분별위0.1267±0.05140화0.1604±0.06153，명현고우정상대조조0.0797±0.02805화0.1263±0.01724，차이균유통계학의의（P<0.05）。결론 TLR8、TLR9재첨예습우환자피손중적표체교정상대조조명현증강，차집중분포우기저층화극세포층。 TLR8、TLR9가능시HPV적식별수체，삼여궤체국부피손항HPV면역응답。
Objective To explore the role of TLR8 and TLR9 on the pathogenesis of condyloma acuminatum (CA) through de-tecting the expressions of TLR8 and TLR9 on skin lesions,and provide reference theory basis in the immunological pathogenesis of CA. Methods An immunohistochemical method using Max vision (Ready-to-use immunohistochemical one-step quickly) was per-formed to detect the expressions of TLR8 and TLR9 in tissue specimens obtained from the CA lesions of 50 patients and from the foreskin of 10 normal human controls. Results In normal skin samples,there was a week expression of TLR8 in the stratum basale,and in the cytoplasm-based,but a negative expression in keratinocytes of almost all epidermal layers except for stratum basale. In contrast,a strong expression of TLR8 in the stratum basale and stratum spinosum,medium expression in the stratum corneum and stratum granulosum in CA lesions. In normal skin samples,TLR9 was strong positive expression in almost all epider-mal layers except for stratum corneum,and in the CA lesions,TLR9 was strong expression in all epidermal layers,some particle aggregation. The second,IPP6.0 color image analysis of quantitative research:in the CA lesions,the relative amounts of protein ex-pression of TLR8 and TLR9 are 0.1267 ±0.05140 and 0.1604 ±0.06153 was significantly higher than that in normal foreskins 0. 0797±0.02805 and 0.1263±0.01724 (all P<0.05). Conclusion The expressions of TLR8 and TLR9 are markedly enhanced in CA lesions compared with normal skin,which may be served as recognition receptors for HPV infection.and involved in the body’s anti-HPV immune response.