江西医药
江西醫藥
강서의약
JIANGXI MEDICAL JOURNAL
2015年
8期
755-757,768
,共4页
郭桃英%陆天炎%陈宗玮%潘玫
郭桃英%陸天炎%陳宗瑋%潘玫
곽도영%륙천염%진종위%반매
Mfn2%卵巢癌%细胞周期%增殖
Mfn2%卵巢癌%細胞週期%增殖
Mfn2%란소암%세포주기%증식
Mfn2%Ovarian cancer%Cell cycle%Proliferation
目的：探讨Mfn2对卵巢癌SKOV3细胞株增殖能力的影响，评价Mfn2在卵巢癌发生发展中的作用。方法体外培养人卵巢癌细胞株SKOV3，通过慢病毒包装Mfn2并转染卵巢癌SKOV3细胞株，将其分为3组：转染Mfn2组（SKOV3/Lenti-Mfn2组）、转染阴性对照组（SKOV3/Lenti-EGFP组）和空白对照组（SKOV3组），Western blot检测Mfn2蛋白表达，流式细胞仪检测细胞周期，分析转染Mfn2后细胞周期的变化。结果转染Mfn2后卵巢癌细胞株呈短梭形改变，片状伪足减少。Western blot检测3组细胞Mfn2蛋白表达，SKOV3/Lenti-Mfn2组较两对照组显著上调，差异有统计学意义（P<0.05）。转染Mfn2后Mfn2表达组（SKOV3/Lenti-Mfn2组）、转染阴性对照组（SKOV3/Lenti-EGFP组）和空白对照组（SKOV3组）S期细胞平均比例分别为（37.4±1.1）％、（43.7±0.8）%和（66.3±0.4）%，而 G1细胞比例分别为（48.1±2.2）%、（43.6±1.0）%和（24.1±2.0）%，Mfn2表达组与其他两组比较，Mfn2表达组的细胞S期增殖指数较低，而停留在G1期的细胞比例则较高，差异有统计学意义（P<0.05）。结论 Mfn2基因过表达后，SKOV3卵巢癌细胞增殖指数降低，表明Mfn2基因上调可以抑制SKOV3细胞的增殖。
目的：探討Mfn2對卵巢癌SKOV3細胞株增殖能力的影響，評價Mfn2在卵巢癌髮生髮展中的作用。方法體外培養人卵巢癌細胞株SKOV3，通過慢病毒包裝Mfn2併轉染卵巢癌SKOV3細胞株，將其分為3組：轉染Mfn2組（SKOV3/Lenti-Mfn2組）、轉染陰性對照組（SKOV3/Lenti-EGFP組）和空白對照組（SKOV3組），Western blot檢測Mfn2蛋白錶達，流式細胞儀檢測細胞週期，分析轉染Mfn2後細胞週期的變化。結果轉染Mfn2後卵巢癌細胞株呈短梭形改變，片狀偽足減少。Western blot檢測3組細胞Mfn2蛋白錶達，SKOV3/Lenti-Mfn2組較兩對照組顯著上調，差異有統計學意義（P<0.05）。轉染Mfn2後Mfn2錶達組（SKOV3/Lenti-Mfn2組）、轉染陰性對照組（SKOV3/Lenti-EGFP組）和空白對照組（SKOV3組）S期細胞平均比例分彆為（37.4±1.1）％、（43.7±0.8）%和（66.3±0.4）%，而 G1細胞比例分彆為（48.1±2.2）%、（43.6±1.0）%和（24.1±2.0）%，Mfn2錶達組與其他兩組比較，Mfn2錶達組的細胞S期增殖指數較低，而停留在G1期的細胞比例則較高，差異有統計學意義（P<0.05）。結論 Mfn2基因過錶達後，SKOV3卵巢癌細胞增殖指數降低，錶明Mfn2基因上調可以抑製SKOV3細胞的增殖。
목적：탐토Mfn2대란소암SKOV3세포주증식능력적영향，평개Mfn2재란소암발생발전중적작용。방법체외배양인란소암세포주SKOV3，통과만병독포장Mfn2병전염란소암SKOV3세포주，장기분위3조：전염Mfn2조（SKOV3/Lenti-Mfn2조）、전염음성대조조（SKOV3/Lenti-EGFP조）화공백대조조（SKOV3조），Western blot검측Mfn2단백표체，류식세포의검측세포주기，분석전염Mfn2후세포주기적변화。결과전염Mfn2후란소암세포주정단사형개변，편상위족감소。Western blot검측3조세포Mfn2단백표체，SKOV3/Lenti-Mfn2조교량대조조현저상조，차이유통계학의의（P<0.05）。전염Mfn2후Mfn2표체조（SKOV3/Lenti-Mfn2조）、전염음성대조조（SKOV3/Lenti-EGFP조）화공백대조조（SKOV3조）S기세포평균비례분별위（37.4±1.1）％、（43.7±0.8）%화（66.3±0.4）%，이 G1세포비례분별위（48.1±2.2）%、（43.6±1.0）%화（24.1±2.0）%，Mfn2표체조여기타량조비교，Mfn2표체조적세포S기증식지수교저，이정류재G1기적세포비례칙교고，차이유통계학의의（P<0.05）。결론 Mfn2기인과표체후，SKOV3란소암세포증식지수강저，표명Mfn2기인상조가이억제SKOV3세포적증식。
Objective To investigate the influence of Mfn2 on the proliferative capability of ovarian cancer SKOV3 cells ,ex-plore their roles in tumorigenesis. Methods The SKOV3 cells were cultured in vitro,lentivirus were used to package Mfn2 and transfect the SKOV3 cells,it can be divided into three groups:transfection Mfn2 group (SKOV3/Lenti-Mfn2 group),transfection negative control group (SKOV3/Lenti-EGFP group) and blank control group (group SKOV3),Western blotting detected Mfn2 pro-tein expression,flow cytometry instrument detected the cell cycle,analyzed the change of cell cycle after transfection Mfn2. Re-sults The SKOV3 cells changed spindle and flake pseudopodia decreased after transfection Mfn2. Western blotting detected Mfn2 protein expression of three groups:SKOV3/Lenti-Mfn2 group was significantly raised than others,the difference was statistically significant(P<0.05). The proportion of cells in S period was (37.4±1.1)%in Mfn2 express group (SKOV3/Lenti-Mfn2 group),(43.7± 0.8)% in transfection negative control group (SKOV3/Lenti-EGFP group),and (66.3±0.4)% in blank control group (group SKOV3). the proportion of cells in G1 period were (48.1±2.2)%、(43.6±1.0)% and (24.1±2.0)%,The proportion of cells in G1 period was higher but lower in S period in SKOV3/Lenti-Mfn2 group than others groups. the difference of two periods were statistically signifi-cant (P<0.05). Conclusion SKOV3 ovarian cancer cell proliferation index decreased after Mfn2 gene Excessive expression ,sug-gested Mfn2 Gene increase can inhibit the proliferation of SKOV3 cells.
