包头医学院学报
包頭醫學院學報
포두의학원학보
JOURNAL OF BAOTOU MEDICAL COLLEGE
2015年
8期
1-2
,共2页
胆碱能拮抗剂%急性尿潴留%膀胱
膽堿能拮抗劑%急性尿潴留%膀胱
담감능길항제%급성뇨저류%방광
Cholinergic antagonists%Acute urinary retention%Bladder
目的：：探讨戊乙奎醚对大鼠急性尿潴留膀胱排空后膀胱组织丙二醛含量和超氧化物歧化酶活性的影响。方法：健康雌性Wistar大鼠56只,随机分为对照组( C 组)和实验组( E 组)；根据膀胱排空后时间点的不同,将 C 组和 E 组大鼠又分别分为排空后0 h( T0)、排空后1 h( T1)、排空后2 h( T2)、排空后3 h( T3)。在实验开始前20 min,E组经腹腔注射盐酸戊乙奎醚2 mg/kg,C组经腹腔注射等容量的生理盐水。 C组和E组均在氯胺酮麻醉下经尿道插入直径l mm的硬膜外导管作为导尿管。经尿管注入生理盐水0.5 mL并结扎,同时经腹腔注射速尿12 mg/kg,5 min后再经腹腔注射乳酸林格液4 mL,潴尿3 h开始排尿,在膀胱排空后各个时间点切取膀胱组织,测定膀胱组织匀浆上清液中丙二醛含量及超氧化物歧化酶活性。结果：与C组比较,E组每个时间点SOD活性均高于C组( P <0.01),MDA含量均低于C组( P <0.01)。结论：盐酸戊乙奎醚提高了膀胱组织的抗氧化能力,减轻了脂质过氧化程度,对大鼠急性尿潴留膀胱排空后膀胱组织的氧化应激反应有一定的抑制作用。
目的：：探討戊乙奎醚對大鼠急性尿潴留膀胱排空後膀胱組織丙二醛含量和超氧化物歧化酶活性的影響。方法：健康雌性Wistar大鼠56隻,隨機分為對照組( C 組)和實驗組( E 組)；根據膀胱排空後時間點的不同,將 C 組和 E 組大鼠又分彆分為排空後0 h( T0)、排空後1 h( T1)、排空後2 h( T2)、排空後3 h( T3)。在實驗開始前20 min,E組經腹腔註射鹽痠戊乙奎醚2 mg/kg,C組經腹腔註射等容量的生理鹽水。 C組和E組均在氯胺酮痳醉下經尿道插入直徑l mm的硬膜外導管作為導尿管。經尿管註入生理鹽水0.5 mL併結扎,同時經腹腔註射速尿12 mg/kg,5 min後再經腹腔註射乳痠林格液4 mL,潴尿3 h開始排尿,在膀胱排空後各箇時間點切取膀胱組織,測定膀胱組織勻漿上清液中丙二醛含量及超氧化物歧化酶活性。結果：與C組比較,E組每箇時間點SOD活性均高于C組( P <0.01),MDA含量均低于C組( P <0.01)。結論：鹽痠戊乙奎醚提高瞭膀胱組織的抗氧化能力,減輕瞭脂質過氧化程度,對大鼠急性尿潴留膀胱排空後膀胱組織的氧化應激反應有一定的抑製作用。
목적：：탐토무을규미대대서급성뇨저류방광배공후방광조직병이철함량화초양화물기화매활성적영향。방법：건강자성Wistar대서56지,수궤분위대조조( C 조)화실험조( E 조)；근거방광배공후시간점적불동,장 C 조화 E 조대서우분별분위배공후0 h( T0)、배공후1 h( T1)、배공후2 h( T2)、배공후3 h( T3)。재실험개시전20 min,E조경복강주사염산무을규미2 mg/kg,C조경복강주사등용량적생리염수。 C조화E조균재록알동마취하경뇨도삽입직경l mm적경막외도관작위도뇨관。경뇨관주입생리염수0.5 mL병결찰,동시경복강주사속뇨12 mg/kg,5 min후재경복강주사유산림격액4 mL,저뇨3 h개시배뇨,재방광배공후각개시간점절취방광조직,측정방광조직균장상청액중병이철함량급초양화물기화매활성。결과：여C조비교,E조매개시간점SOD활성균고우C조( P <0.01),MDA함량균저우C조( P <0.01)。결론：염산무을규미제고료방광조직적항양화능력,감경료지질과양화정도,대대서급성뇨저류방광배공후방광조직적양화응격반응유일정적억제작용。
Objective:To investigate the effects of penehyclidine hydrochloride on the content of malondialdehyde and activities of superoxide dismutase in evacuating bladder tissue after acute urinary retention of wistar rats. Methods:Fifty-six healthy female Wistar rats were randomly di-vided into 2 groups:control group ( group C) and experiment group ( group E) . According to different time points after bladder evacuation,group C and group E were respectively divided into group 0h(T0), group 1 h(T1), group 2 h (T2), group 3 h (T3)after emptying. Twenty minutes be-fore anesthesia,group E was injected intraperitoneally with penehyclidine hydrochloride 2 mg/kg and Group C equi -volume normal saline. In group C and E, epidural catheter l mm in diameter was inserted and fixed into bladder via urethra under ketamine anesthesia. The normal saline 0. 5 mL was injected via catheter and then catheter was deligated while 12 mg/kg furosemide was injected intraperitoneally. Five minutes later lactic acid Ringer's solution 4ml was injected. Urinating started 3h after urine retention and empty bladder tissue was cut at different time points after uri-nating. The content of malondialdehyde and activities of superoxide dismutase in the supernatant of bladder tissue homogenate were determined. Re-sults:Compared with group C, the SOD activity of group E was higher than that in group C ( P <0. 01) and the MDA level was lower than that in group C at every time point ( P <0. 01). Conclusion:Penehyclidine hydrochloride has elevated antioxidant capacity of the bladder tissue and re-lieved lipid peroxidation level and it has an invariably depressant effect on oxidative stress of evacuating bladder tissue after acute urinary retention of rats.
