口腔医学
口腔醫學
구강의학
STOMATOLOGY
2015年
8期
614-618
,共5页
岑胜丹%陈利娇%胡招玲%邓辉%胡荣党
岑勝丹%陳利嬌%鬍招玲%鄧輝%鬍榮黨
잠성단%진리교%호초령%산휘%호영당
牙龈卟啉单胞菌%脂多糖%THP-1 单核细胞%CD36%LOX-1
牙齦卟啉單胞菌%脂多糖%THP-1 單覈細胞%CD36%LOX-1
아간계람단포균%지다당%THP-1 단핵세포%CD36%LOX-1
Porphyromonas gingivalis%lipopolysaccharide%THP-1 monocytes%CD36%LOX-1
目的:该文以THP -1源性巨噬细胞为研究对象,研究牙龈卟啉单胞菌脂多糖(P. g - LPS)对巨噬细胞内胆固醇代谢关键分子CD36、LOX -1表达的影响。方法以160 nmol / L 佛波酯(PMA)诱导THP -1单核细胞48 h,使其分化为巨噬细胞。建立P. g - LPS 与THP -1源性巨噬细胞共孵育的体外模型,在负荷50μg / mL 低密度脂蛋白(LDL)的条件下,分别用浓度为0.1、1.0、10.0μg / mL P. g - LPS 与巨噬细胞共孵育48 h;1μg / mL P. g - LPS 与巨噬细胞共孵育24、48和72 h。采用实时荧光定量PCR 和Western blot 方法探讨巨噬细胞胆固醇代谢关键分子CD36和LOX -1表达变化。结果与空白对照组相比,随着P. g - LPS 浓度的增加和1μg / mL P. g - LPS 孵育时间的延长,巨噬细胞内CD36 mRNA 和蛋白表达均逐渐下降;LOX-1mRNA 和蛋白表达无明显变化。结论 P. g - LPS 可促进巨噬细胞CD36的表达,对LOX -1表达无影响,从而可能对巨噬细胞脂质代谢产生影响。
目的:該文以THP -1源性巨噬細胞為研究對象,研究牙齦卟啉單胞菌脂多糖(P. g - LPS)對巨噬細胞內膽固醇代謝關鍵分子CD36、LOX -1錶達的影響。方法以160 nmol / L 彿波酯(PMA)誘導THP -1單覈細胞48 h,使其分化為巨噬細胞。建立P. g - LPS 與THP -1源性巨噬細胞共孵育的體外模型,在負荷50μg / mL 低密度脂蛋白(LDL)的條件下,分彆用濃度為0.1、1.0、10.0μg / mL P. g - LPS 與巨噬細胞共孵育48 h;1μg / mL P. g - LPS 與巨噬細胞共孵育24、48和72 h。採用實時熒光定量PCR 和Western blot 方法探討巨噬細胞膽固醇代謝關鍵分子CD36和LOX -1錶達變化。結果與空白對照組相比,隨著P. g - LPS 濃度的增加和1μg / mL P. g - LPS 孵育時間的延長,巨噬細胞內CD36 mRNA 和蛋白錶達均逐漸下降;LOX-1mRNA 和蛋白錶達無明顯變化。結論 P. g - LPS 可促進巨噬細胞CD36的錶達,對LOX -1錶達無影響,從而可能對巨噬細胞脂質代謝產生影響。
목적:해문이THP -1원성거서세포위연구대상,연구아간계람단포균지다당(P. g - LPS)대거서세포내담고순대사관건분자CD36、LOX -1표체적영향。방법이160 nmol / L 불파지(PMA)유도THP -1단핵세포48 h,사기분화위거서세포。건립P. g - LPS 여THP -1원성거서세포공부육적체외모형,재부하50μg / mL 저밀도지단백(LDL)적조건하,분별용농도위0.1、1.0、10.0μg / mL P. g - LPS 여거서세포공부육48 h;1μg / mL P. g - LPS 여거서세포공부육24、48화72 h。채용실시형광정량PCR 화Western blot 방법탐토거서세포담고순대사관건분자CD36화LOX -1표체변화。결과여공백대조조상비,수착P. g - LPS 농도적증가화1μg / mL P. g - LPS 부육시간적연장,거서세포내CD36 mRNA 화단백표체균축점하강;LOX-1mRNA 화단백표체무명현변화。결론 P. g - LPS 가촉진거서세포CD36적표체,대LOX -1표체무영향,종이가능대거서세포지질대사산생영향。
Objective To explore the effect of porphyromonas gingivalis lipopolysaccharides (P. g-LPS)on the expression of CD36 and LOX-1 macrophages derived from THP-1. Methods THP-1 cells were cultured in the presence of 160 nmol / L phorbol 12-myris-tate 13-acetate (PMA)for 48 h. An co-cultured in vitro model of P. g-LPS and THP-1 macrophage cells was constructed. In the pres-ence of LDL (50 μg / ml),macrophage cells were incubated with P. g-LPS (0. 1,1,10 μg / ml)for 48 h or with 1μg / ml P. g-LPS for 24,48,72h. The expression of CD36,LOX-1 at mRNA and protein levels was determined by real time PCR and Western blot. Results THP-1 cells were differentiated into macrophages after the addition of PMA for 48h. P. g-LPS down-regulated the mRNA and protein ex-pression of CD36 as concentration increased and time extended in the presence of LDL (P < 0. 05). However,P. g-LPS had no effect on the expression of LOX-1 at mRNA and protein levels compared with control group. Conclusions P. g-LPS down-regulated CD36,but had no effect on the expression of LOX-1,which may contribute to the excessive accumulation of cholesteryl esters and conversion to foam cells in macrophages.