CAJ | 학술논문

目的：分析牙龈卟啉单胞菌及其毒力因子牙龈蛋白K 在青少年正畸治疗早期的变化。方法随机收集青少年正畸牙龈正常者45例，分别取矫治器戴入前，戴入后1、2、3、6个月龈沟液标本，应用16S rDNA PCR 技术检测各样本中的牙龈卟啉单胞菌及其毒力因子牙龈蛋白K；采用SPSS17．0软件包对数据进行统计分析，牙龈卟啉单胞菌及牙龈蛋白K 各组间检出率比较用χ2检验；牙龈卟啉单胞菌、牙龈蛋白K 的检出率与牙龈炎症之间的关系用Spearman 等级相关分析。结果牙龈卟啉单胞菌在矫治前与戴入后1个月有显著性差异（P ＜0．05），与2个月、3个月之间有极显著性差异（P ＜0．01）；戴入后1个月与2个月之间有显著性差异（P ＜0．05）；牙龈蛋白K 在矫治器戴入前与戴入后1个月、6个月之间无显著性差异，与戴入后2个月、3个月之间有显著性差异（P ＜0．05）；从第2个月开始牙龈卟啉单胞菌与牙龈蛋白K 的检出率下降，到6个月时检出率已基本接近矫治器戴入前。结论青少年正畸治疗过程中在矫治器戴入早期可引发牙龈卟啉单胞菌及牙龈蛋白K 的增加，出现牙龈炎症反应，当增加到一定时间即2个月后开始逐渐下降，到6个月时已基本接近矫治器戴入前。
목적：분석아간계람단포균급기독력인자아간단백K 재청소년정기치료조기적변화。방법수궤수집청소년정기아간정상자45례，분별취교치기대입전，대입후1、2、3、6개월간구액표본，응용16S rDNA PCR 기술검측각양본중적아간계람단포균급기독력인자아간단백K；채용SPSS17．0연건포대수거진행통계분석，아간계람단포균급아간단백K 각조간검출솔비교용χ2검험；아간계람단포균、아간단백K 적검출솔여아간염증지간적관계용Spearman 등급상관분석。결과아간계람단포균재교치전여대입후1개월유현저성차이（P ＜0．05），여2개월、3개월지간유겁현저성차이（P ＜0．01）；대입후1개월여2개월지간유현저성차이（P ＜0．05）；아간단백K 재교치기대입전여대입후1개월、6개월지간무현저성차이，여대입후2개월、3개월지간유현저성차이（P ＜0．05）；종제2개월개시아간계람단포균여아간단백K 적검출솔하강，도6개월시검출솔이기본접근교치기대입전。결론청소년정기치료과정중재교치기대입조기가인발아간계람단포균급아간단백K 적증가，출현아간염증반응，당증가도일정시간즉2개월후개시축점하강，도6개월시이기본접근교치기대입전。
Objective To explore the changes of porphyromonasgingivalis and its virulence factor gingipain K during orthodontic treatment. Methods Totally 45 orthodontic healthy teenagers were randomly chosen from the orthodontics department. Givgival crevicu-lar fluid before orthodontic treatment,in the first,second,third and sixth month after treatment were collected simultaneouslyat each time point. 16S rDNA PCR technique was used to detect porphyromonasgingivalis and its virulence factor gingipain K. SPSS 17. 0 soft-ware package was used to analyze the data. The detection rates of porphyromonasgingivalis and gingipain K between groups were com-pared by χ2 . The correlation of the detection rates and gingival inflammation was analyzed by Spearman. Results Porphyromonasgingi-valis before treament was significantly different form that in the first month after treatment (P < 0. 05),and demonstrated extremely sig-nificant difference from that in the second and third month(P < 0. 01). Difference was also found between the first month after treat-ment and the second month after treatment. As for gingipain K,there was no difference before treatment,the first month after treatment and the sixth month after treatment,while statistical difference was found between the second month after treatment and the third month after treatment(P < 0. 05). Since the third month after treatment,the rate of porphyromonasgingivalis and gingipain K had decreased, and in the sixth month after treatment,it approached the level before treatment. Conclusions During the early process of orthodontic treatment,porphyromonasgingivalis and gingipain K may increase and can lead to inflammatory reaction. Started from the second month after treatment,it began to decline and it basically approached the level before treatment in the sixth month.