CAJ | 학술논문

目的：神经细胞黏附分子 L1(L1 CAM)在肝癌中的表达及其与临床预后的关系。方法对110例肝癌样本用Western blot 及 qRT-PCR 方法检测 L1CAM 蛋白和 mRNA 的表达，分析了 L1CAM 表达与肝癌患者总体生存率的关系。结果肝癌组织 L1CAM 蛋白、mRNA 表达水平显著高于对应非肿瘤组织(P <0.01)；在中-低分化肝癌患者 L1CAM 表达量高于高分化肝癌患者(P <0.01)；L1CAM mRNA 的表达与肿瘤的分化程度、TNM 分期密切相关(P <0.05)；采用 Kaplan-Meier 法生存分析，高表达 L1CAM 患者预后差(P <0.01)。结论L1CAM 与肝癌的发生、发展具有相关性，或许可作为评估肝癌患者预后的指标之一。
목적：신경세포점부분자 L1(L1 CAM)재간암중적표체급기여림상예후적관계。방법대110례간암양본용Western blot 급 qRT-PCR 방법검측 L1CAM 단백화 mRNA 적표체，분석료 L1CAM 표체여간암환자총체생존솔적관계。결과간암조직 L1CAM 단백、mRNA 표체수평현저고우대응비종류조직(P <0.01)；재중-저분화간암환자 L1CAM 표체량고우고분화간암환자(P <0.01)；L1CAM mRNA 적표체여종류적분화정도、TNM 분기밀절상관(P <0.05)；채용 Kaplan-Meier 법생존분석，고표체 L1CAM 환자예후차(P <0.01)。결론L1CAM 여간암적발생、발전구유상관성，혹허가작위평고간암환자예후적지표지일。
Objective To investigate the clinical significance and prognostic value of PKD3 expression in human hepatocellu-lar carcinoma (HCC)after hepatectomy.Methods We analyzed mRNA expression of L1CAM in 1 10 HCCs by quantitative real-time PCR (qRT-PCR)and western blot,and the relationship among the overall survival of HCCs.Results The relative protein and mRNA expression level of L1CAM was up-regulated in HCCs comparing with adjacent non tumor liver tissues (P <0.01).L1CAM expression in the well-differentiated group was higher than that in the poor-differentiated group (P < 0.01 ).The expression of L1CAM mRNA was significantly correlated with tumor differentiation and TNM stage (P <0.05).The prognosis of patients with high expression L1CAM was poor (P <0.01).Conclusion L1CAM expression is related to occurrence and development of HCCs and may predict the prognosis of HCCs after hepatectomy.