CAJ | 학술논문

目的：研究胶质细胞系源性神经营养因子(GDNF)促进人胶质瘤细胞增殖的机制。方法将胶质瘤样本分为低级别胶质瘤组和高级别胶质瘤组,脑挫裂伤患者样本作为正常对照组,每组各12例；将胶质瘤细胞系 C6细胞分为细胞对照组、牛血清蛋白(BSA)组和 GDNF 组。CCK-8实验检测细胞增殖率,蛋白免疫印迹(Western blot)法检测各组 AKT、p-AKT、β-catenin 和p-β-catenin 的表达。结果与正常对照组相比,胶质瘤组 AKT、p-AKT、β-catenin 和 p-β-catenin 的蛋白水平显著增加(P <0.05),且高级别胶质瘤组的蛋白水平明显高于低级别胶质瘤组(P <0.05)。CCK-8检测 C6胶质瘤细胞实验中,与细胞对照组相比, GDNF 组细胞增殖率明显增高(P <0.05),Akt 表达水平没有明显变化,而 p-Akt、β-catenin 和 p-β-catenin 的蛋白表达均显著增加(P <0.05)。结论GDNF 可能通过上调胶质瘤细胞 p-AKT、β-catenin 和 p-β-catenin 来促进胶质瘤细胞的增殖。
목적：연구효질세포계원성신경영양인자(GDNF)촉진인효질류세포증식적궤제。방법장효질류양본분위저급별효질류조화고급별효질류조,뇌좌렬상환자양본작위정상대조조,매조각12례；장효질류세포계 C6세포분위세포대조조、우혈청단백(BSA)조화 GDNF 조。CCK-8실험검측세포증식솔,단백면역인적(Western blot)법검측각조 AKT、p-AKT、β-catenin 화p-β-catenin 적표체。결과여정상대조조상비,효질류조 AKT、p-AKT、β-catenin 화 p-β-catenin 적단백수평현저증가(P <0.05),차고급별효질류조적단백수평명현고우저급별효질류조(P <0.05)。CCK-8검측 C6효질류세포실험중,여세포대조조상비, GDNF 조세포증식솔명현증고(P <0.05),Akt 표체수평몰유명현변화,이 p-Akt、β-catenin 화 p-β-catenin 적단백표체균현저증가(P <0.05)。결론GDNF 가능통과상조효질류세포 p-AKT、β-catenin 화 p-β-catenin 래촉진효질류세포적증식。
Objective To study the mechanism that glial cell line-derived neurotrophic factor (GDNF)promotes human glio-ma cells proliferation.Methods We divided glioma samples into two groups,including low-grade glioma group and high-grade glio-ma group,while cerebral contusion patients were treated as the control group,12 cases in each group.C6 glioma cell lines were di-vided into three groups,such as GDNF group,BSA(bovine serum albumin)group and control group.CCK-8 (cell counting kit-8) was used to detect the cell proliferation,while Western blot was used to detect the expression of AKT,p-AKT,β-catenin and p-β-catenin in each group.Results Comparing with the control group,the expression levels of AKT,p-AKT,β-catenin and p-β-catenin in glioma group had a significantly increased (P <0.05).Meanwhile,the high-grade gliomas group also had a significant increase in those more than low-grade gliomas group (P <0.05).CCK-8 test showed that the cell proliferation in GDNF group was significant-ly higher than the control group (P <0.05),and the expression levels of p-AKT,β-catenin and p-β-catenin proteins all had a signifi-cant increase (P <0.05).However,the expression level of AKT had no obvious difference.Conclusion GDNF might promote the proliferation of glioma cells by up-regulating the expression of p-AKT,β-catenin and p-β-catenin.