重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2015年
22期
3025-3029
,共5页
冯潜%石世代%周勇%李恩亮%吴荣寿%李科浩%邬林泉
馮潛%石世代%週勇%李恩亮%吳榮壽%李科浩%鄔林泉
풍잠%석세대%주용%리은량%오영수%리과호%오림천
KLF4%基质金属蛋白酶 9%肝肿瘤%侵袭%迁移
KLF4%基質金屬蛋白酶 9%肝腫瘤%侵襲%遷移
KLF4%기질금속단백매 9%간종류%침습%천이
KLF4%matrix metalloproteinase 9%liver neoplasms%invasion%migration
目的：研究Krüppel 样因子4(KLF4)和基质金属蛋白酶9(MMP9)在原发性肝癌中的表达情况,探讨 KLF4调节MMP9对原发性肝癌细胞侵袭和迁移能力的影响。方法收集原发性肝癌手术患者癌和癌旁组织标本,通过免疫组织化学、实时荧光定量 PCR 和免疫印迹试验(Western blot)检测50例肝癌组织及对应癌旁组织中 KLF4和 MMP9的表达情况；构建重组质粒,上调肝癌细胞(HepG2细胞系)的 KLF4表达,检测 MMP9 mRNA 及蛋白水平的表达情况；转染后的 HepG2细胞运用 Tran-swell 侵袭试验和划痕试验观察侵袭和迁移能力的变化。结果相比癌旁组织,KLF4在肝癌组织中的表达明显降低(P <0.05),而 MMP9的表达明显增高(P <0.05)。通过构建重组质粒,上调 KLF4的表达,发现 MMP9的 mRNA 和蛋白表达均降低,并影响 HepG2细胞的侵袭和迁移能力。结论在原发性肝癌中,KLF4低表达,而 MMP9高表达。在肝癌细胞中上调 KLF4表达可引起 MMP9表达下降,进而抑制肝癌细胞的侵袭和迁移。
目的：研究Krüppel 樣因子4(KLF4)和基質金屬蛋白酶9(MMP9)在原髮性肝癌中的錶達情況,探討 KLF4調節MMP9對原髮性肝癌細胞侵襲和遷移能力的影響。方法收集原髮性肝癌手術患者癌和癌徬組織標本,通過免疫組織化學、實時熒光定量 PCR 和免疫印跡試驗(Western blot)檢測50例肝癌組織及對應癌徬組織中 KLF4和 MMP9的錶達情況；構建重組質粒,上調肝癌細胞(HepG2細胞繫)的 KLF4錶達,檢測 MMP9 mRNA 及蛋白水平的錶達情況；轉染後的 HepG2細胞運用 Tran-swell 侵襲試驗和劃痕試驗觀察侵襲和遷移能力的變化。結果相比癌徬組織,KLF4在肝癌組織中的錶達明顯降低(P <0.05),而 MMP9的錶達明顯增高(P <0.05)。通過構建重組質粒,上調 KLF4的錶達,髮現 MMP9的 mRNA 和蛋白錶達均降低,併影響 HepG2細胞的侵襲和遷移能力。結論在原髮性肝癌中,KLF4低錶達,而 MMP9高錶達。在肝癌細胞中上調 KLF4錶達可引起 MMP9錶達下降,進而抑製肝癌細胞的侵襲和遷移。
목적：연구Krüppel 양인자4(KLF4)화기질금속단백매9(MMP9)재원발성간암중적표체정황,탐토 KLF4조절MMP9대원발성간암세포침습화천이능력적영향。방법수집원발성간암수술환자암화암방조직표본,통과면역조직화학、실시형광정량 PCR 화면역인적시험(Western blot)검측50례간암조직급대응암방조직중 KLF4화 MMP9적표체정황；구건중조질립,상조간암세포(HepG2세포계)적 KLF4표체,검측 MMP9 mRNA 급단백수평적표체정황；전염후적 HepG2세포운용 Tran-swell 침습시험화화흔시험관찰침습화천이능력적변화。결과상비암방조직,KLF4재간암조직중적표체명현강저(P <0.05),이 MMP9적표체명현증고(P <0.05)。통과구건중조질립,상조 KLF4적표체,발현 MMP9적 mRNA 화단백표체균강저,병영향 HepG2세포적침습화천이능력。결론재원발성간암중,KLF4저표체,이 MMP9고표체。재간암세포중상조 KLF4표체가인기 MMP9표체하강,진이억제간암세포적침습화천이。
Objective To investigate the effects of Krüppel like factor 4 (KLF4)on matrix metalloproteinase 9 (MMP9)ex-pression in hepatocellular carcinoma(HCC).Methods A total of 50 primary hepatocellular carcinoma samples and their correspond-ing adjacent tissues specimens were collected.The expression of KLF4 and MMP9 were detected by IHC,Western blot and qRT-PCR.After KLF4 gene was transfected into hepatocellular carcinoma cell line (HepG2 cell line),the expressions of KLF4 and MMP9 were conformed by qRT-PCR and Western blot.Migration and invasion of HepG2 cell line transfected by KLF4 were detec-ted by wound-healing assay and invasion assay.Results Compared to corresponding adjacent tissues,The expression of KLF4 was significantly lower in HCCs(P <0.05),and MMP9 expression was remarkably higher in HCCs(P <0.05).KLF4 over-expression inhibited the expression of MMP9 on the protein and mRNA levels.Wound-healing assay and invasion assay confirmed that KLF4 regulated cell invasion and migration through regulating MMP9 expression.Conclusion KLF4 showed low expression in HCCs,and MMP9 was overexpressed.Up-regulation of KLF4 could decrease the expression of MMP9 in HepG2 cell line,which inhibited inva-sion and migration.
