CAJ | 학술논문

目的：探索利用CD3、CD28抗体对人外周血单个核细胞(PBMC)体外激活转化增强作用，采用不同包被方法及抗体浓度，以期获得一种更有效的包被方法。方法利用人外周血单个核细胞分离技术、体外细胞培养技术及流式细胞术(FMC)，以 CD3抗体固相包被、CD28抗体不同浓度固相包被或悬浮加入，计算经12 d 培养获得成熟 CIK 细胞的数量，测定不同包被方法刺激培养后的细胞表型的变化。结果培养后 C 组中 CD4+细胞百分比明显低于 A 组，差异有统计学意义(P <0.05)。C 组中CD8+细胞所占比例高于 A、B 组(P <0.05)。C 组的 T4/T8比值与 A、B 组差异有统计学意义(P <0.05)。B 组 NK 细胞含量与C 组差异有统计学意义(P <0.05)。CD25+细胞在 C 组中所占比例低于 A 组(P <0.01)。结论CD3抗体固相包被及 CD28抗体悬浮加入组合对人外周血细胞的刺激增强作用强于 CD3抗体固相包被 CD28+抗体固相包被组合。
목적：탐색이용CD3、CD28항체대인외주혈단개핵세포(PBMC)체외격활전화증강작용，채용불동포피방법급항체농도，이기획득일충경유효적포피방법。방법이용인외주혈단개핵세포분리기술、체외세포배양기술급류식세포술(FMC)，이 CD3항체고상포피、CD28항체불동농도고상포피혹현부가입，계산경12 d 배양획득성숙 CIK 세포적수량，측정불동포피방법자격배양후적세포표형적변화。결과배양후 C 조중 CD4+세포백분비명현저우 A 조，차이유통계학의의(P <0.05)。C 조중CD8+세포소점비례고우 A、B 조(P <0.05)。C 조적 T4/T8비치여 A、B 조차이유통계학의의(P <0.05)。B 조 NK 세포함량여C 조차이유통계학의의(P <0.05)。CD25+세포재 C 조중소점비례저우 A 조(P <0.01)。결론CD3항체고상포피급 CD28항체현부가입조합대인외주혈세포적자격증강작용강우 CD3항체고상포피 CD28+항체고상포피조합。
Objective To explore the enhancing effect of monoclonal antibody coated with anti-human CD3 and CD28 on acti-vation and transformation of peripheral blood mononuclear cell (PBMC)in vitro.Methods Human peripheral blood mononuclear cells were separated.Cells was cultured in vitro,and determined by flow cytometry.The solid phase with CD3 and CD28 antibody was coated and added in.The mature CIK cells were obtained after 12 days culturing.Results The CD4 + cells was lower in group C than those in group A(P <0.05).The CD8 + cells was higher in group C than that in group A and B(P <0.05).There was signifi-cant difference of T4/T8 between group C and group A and B(P <0.05 ).There was significant difference of NK cells between group B and group C(P <0.05).The CD25 + cells was lower in group C than that in group A (P <0.01).Conclusion CD3 antibody solid coated combined with CD28 antibody added to the suspension has more strong activation than both CD3 antibody and CD28 antibody solid coating on peripheral blood mononuclear cell.