白血病·淋巴瘤
白血病·淋巴瘤
백혈병·림파류
JOURNAL OF LEUKEMIA & LYMPHOMA
2015年
7期
437-439
,共3页
苏小丽%冯苗娟%侯莉萍%朱华锋%常子维%田志强%于书春%于岩%高广勋
囌小麗%馮苗娟%侯莉萍%硃華鋒%常子維%田誌彊%于書春%于巖%高廣勛
소소려%풍묘연%후리평%주화봉%상자유%전지강%우서춘%우암%고엄훈
原发性骨髓纤维化%病理学,临床%JAK2 V617F基因突变
原髮性骨髓纖維化%病理學,臨床%JAK2 V617F基因突變
원발성골수섬유화%병이학,림상%JAK2 V617F기인돌변
Primary myelofibrosis%Pathology,clinic%JAK2 V617F gene mutation
目的 探讨原发性骨髓纤维化(PMF)患者的骨髓病理特点,检测JAK2 V617F突变的发生率,分析JAK2 V617F突变对PMF患者血细胞及骨髓纤维组织增生的影响.方法 37例PMF患者的骨髓切片经苏木精-吉姆萨-伊红(HGE)染色及Gomori染色后进行形态学研究,采用荧光定量PCR技术检测患者骨髓中JAK2 V617F基因突变情况.结果 37例PMF患者中JAK2 V617F基因突变阳性率为54％(20/37).突变阳性组患者白细胞计数和血小板计数分别为(12.79±3.54)×109/L和(312.47±203.81)×109/L,高于突变阴性组的(4.22±2.07)×109/L和(136.43±104.87)×109/L,差异均有统计学意义(t=3.158,P=0.025;t=2.623,P=0.046),但两组患者血红蛋白数和纤维组织增生程度比较,差异均无统计学意义(均P＞0.05).结论 JAK2 V617F基因突变检测对PMF,特别是白细胞计数和血小板计数增高的PMF诊断有一定意义.
目的 探討原髮性骨髓纖維化(PMF)患者的骨髓病理特點,檢測JAK2 V617F突變的髮生率,分析JAK2 V617F突變對PMF患者血細胞及骨髓纖維組織增生的影響.方法 37例PMF患者的骨髓切片經囌木精-吉姆薩-伊紅(HGE)染色及Gomori染色後進行形態學研究,採用熒光定量PCR技術檢測患者骨髓中JAK2 V617F基因突變情況.結果 37例PMF患者中JAK2 V617F基因突變暘性率為54％(20/37).突變暘性組患者白細胞計數和血小闆計數分彆為(12.79±3.54)×109/L和(312.47±203.81)×109/L,高于突變陰性組的(4.22±2.07)×109/L和(136.43±104.87)×109/L,差異均有統計學意義(t=3.158,P=0.025;t=2.623,P=0.046),但兩組患者血紅蛋白數和纖維組織增生程度比較,差異均無統計學意義(均P＞0.05).結論 JAK2 V617F基因突變檢測對PMF,特彆是白細胞計數和血小闆計數增高的PMF診斷有一定意義.
목적 탐토원발성골수섬유화(PMF)환자적골수병리특점,검측JAK2 V617F돌변적발생솔,분석JAK2 V617F돌변대PMF환자혈세포급골수섬유조직증생적영향.방법 37례PMF환자적골수절편경소목정-길모살-이홍(HGE)염색급Gomori염색후진행형태학연구,채용형광정량PCR기술검측환자골수중JAK2 V617F기인돌변정황.결과 37례PMF환자중JAK2 V617F기인돌변양성솔위54％(20/37).돌변양성조환자백세포계수화혈소판계수분별위(12.79±3.54)×109/L화(312.47±203.81)×109/L,고우돌변음성조적(4.22±2.07)×109/L화(136.43±104.87)×109/L,차이균유통계학의의(t=3.158,P=0.025;t=2.623,P=0.046),단량조환자혈홍단백수화섬유조직증생정도비교,차이균무통계학의의(균P＞0.05).결론 JAK2 V617F기인돌변검측대PMF,특별시백세포계수화혈소판계수증고적PMF진단유일정의의.
Objective To investigate the pathological features of the bone marrow and the mutation of JAK2 V617F gene in patients with primary myelofibrosis (PMF),and analyze the effects of JAK2 V617F mutation on myelofibrosis and the number of hemocytes in patients with PMF.Methods The morphological features of 37 patients with PMF were studied by HGE and Gomori staining.The mutation of JAK2 V617F gene in marrow samples from patients was detected by fluorescence quantitative PCR.Results The positive rate of JAK2 V617F gene mutation in 37 patients with PMF was 54 ％ (20/37).JAK2 V617F positive patients had higher counts of white blood cell and platelet in peripheral blood [(12.79±3.54) × 109/L and (312.47± 203.81) × 109/L] than JAK2 V617F negative patients [(4.22±2.07) × 109/L and (136.43±104.87) × 109/L] (t =3.158,P =0.025;t =2.623,P =0.046).The two groups had no significant difference between the counts of hemoglobin and the level of fiber hyperplasy in bone marrow (both P ＞ 0.05).Conclusion JAK2 V617F gene mutation detection is important for diagnosis of PMF,especially those with high counts of white blood cell and platelet.
